Results 1 to 10 of about 11,654 (172)

Cytoplasmic Dynein Promotes HIV-1 Uncoating [PDF]

Viruses, 2014
Retroviral capsid (CA) cores undergo uncoating during their retrograde transport (toward the nucleus), and/or after reaching the nuclear membrane. However, whether HIV-1 CA core uncoating is dependent upon its transport is not understood.
Lionel Berthoux
exaly   +5 more sources

Dengue Virus Genome Uncoating Requires Ubiquitination [PDF]

mBio, 2016
The process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. Flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, Zika, and West Nile ...
Byk, Laura Andrea, de Maio, Federico Andres, Gamarnik, Andrea Vanesa, Gebhard, Leopoldo German, Iglesias, Nestor Gabriel, Rossi, Mario   +5 more
core   +12 more sources

HIV-1 Uncoating and Nuclear Import Precede the Completion of Reverse Transcription in Cell Lines and in Primary Macrophages

Viruses, 2020
An assembly of capsid proteins (CA) form the mature viral core enclosing the HIV-1 ribonucleoprotein complex. Discrepant findings have been reported regarding the cellular sites and the extent of core disassembly (uncoating) in infected cells.
Ashwanth C Francis, Gregory B Melikyan
exaly   +3 more sources

IP6 Regulation of HIV Capsid Assembly, Stability, and Uncoating

Viruses, 2018
The mechanisms that drive formation of the HIV capsid, first as an immature particle and then as a mature protein shell, remain incompletely understood.
Robert A Dick, Donna L Mallery, Leo C James   +2 more
exaly   +3 more sources

The Role of Capsid in HIV-1 Nuclear Entry

Viruses, 2021
HIV-1 can infect non-dividing cells. The nuclear envelope therefore represents a barrier that HIV-1 must traverse in order to gain access to the host cell chromatin for integration.
Anabel Guedán, Eve R. Caroe, Genevieve C. R. Barr, Kate N. Bishop   +3 more
doaj   +1 more source

Fluorescent image analysis of HIV-1 and HIV-2 uncoating kinetics in the presence of old world monkey TRIM5α. [PDF]

PLoS ONE, 2015
Uncoating of Human Immunodeficiency Virus type 1 (HIV-1) and type 2 (HIV-2) conical cores is an important early step for establishment of infection.
Eri Takeda, Ken Kono, Amy E Hulme, Thomas J Hope, Emi E Nakayama, Tatsuo Shioda   +5 more
doaj   +1 more source

Overlap Intensity: An ImageJ Macro for Analyzing the HIV-1 In Situ Uncoating Assay

Viruses, 2021
Capsid uncoating is at the crossroads of early steps in HIV-1 replication. In recent years, the development of novel assays has expanded how HIV-1 uncoating can be studied.
Zachary Ingram, Hannah Matheney, Emma Wise, Courtney Weatherford, Amy E. Hulme   +4 more
doaj   +1 more source

Characterization of HIV-1 uncoating in human microglial cell lines

Virology Journal, 2020
Background After viral fusion with the cell membrane, the conical capsid of HIV-1 disassembles by a process called uncoating. Previously we have utilized the CsA washout assay, in which TRIM-CypA mediated restriction of viral replication is used to ...
Zachary Ingram, Melanie Taylor, Glister Okland, Richard Martin, Amy E. Hulme   +4 more
doaj   +1 more source

You can keep your coat on

eLife, 2021
High-resolution imaging techniques reveal new insights into the actions of the retrovirus HIV-1 inside host cells.
Gregory J Bedwell, Alan N Engelman
doaj   +1 more source

A spatio-temporal analysis of matrix protein and nucleocapsid trafficking during vesicular stomatitis virus uncoating. [PDF]

PLoS Pathogens, 2010
To study VSV entry and the fate of incoming matrix (M) protein during virus uncoating we used recombinant viruses encoding M proteins with a C-terminal tetracysteine tag that could be fluorescently labeled using biarsenical (Lumio) compounds.
Chad E Mire, Judith M White, Michael A Whitt   +2 more
doaj   +1 more source