Results 1 to 10 of about 1,031,121 (258)

The Host Cell Metabolite Inositol Hexakisphosphate Promotes Efficient Endogenous HIV-1 Reverse Transcription by Stabilizing the Viral Capsid

open access: yesmBio, 2020
A defining activity of retroviruses is reverse transcription, the process by which the viral genomic RNA is converted into the double-stranded DNA required for virus replication.
Jordan Jennings   +4 more
doaj   +3 more sources

Initiation of HIV Reverse Transcription

open access: yesViruses, 2010
Reverse transcription of retroviral genomes into double stranded DNA is a key event for viral replication. The very first stage of HIV reverse transcription, the initiation step, involves viral and cellular partners that are selectively packaged into the
Roland Marquet   +2 more
doaj   +3 more sources

Strand transfer and elongation of HIV-1 reverse transcription is facilitated by cell factors in vitro.

open access: yesPLoS ONE, 2010
Recent work suggests a role for multiple host factors in facilitating HIV-1 reverse transcription. Previously, we identified a cellular activity which increases the efficiency of HIV-1 reverse transcription in vitro.
David Warrilow   +2 more
doaj   +3 more sources

Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription

open access: yesBioTechniques, 2012
Primer-independent cDNA synthesis during reverse transcription hinders quantitative analysis of bidirectional mRNA synthesis in eukaryotes as well as in cells infected with RNA viruses.
Lin Feng   +10 more
doaj   +3 more sources

Reverse transcription progression and genome length regulate HIV-1 core elasticity and disassembly. [PDF]

open access: yesPLoS Pathogens
The structural and mechanical properties of the HIV-1 core are critical for successful infection, balancing stability for early replication and controlled disassembly for genome release.
Akshay Deshpande   +4 more
doaj   +2 more sources

Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]

open access: yesArchives of Razi Institute, 2020
Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
doaj   +1 more source

Epidemiological and molecular study of Rotavirus infection among human and animal in Karbala and Basrah provinces [PDF]

open access: yesIraqi Journal of Veterinary Sciences, 2021
This study was aimed to determine the prevalence of rotaviruses in Karbala and Basrah provinces, the genetic reassortment of human and animal rotavirus strains and the novel strains.
Faten K. Aldawmy   +2 more
doaj   +1 more source

The HIV-1 capsid and reverse transcription

open access: yesRetrovirology, 2021
The viral capsid plays a key role in HIV-1 reverse transcription. Recent studies have demonstrated that the small molecule IP6 dramatically enhances reverse transcription in vitro by stabilizing the viral capsid.
Christopher Aiken, Itay Rousso
doaj   +1 more source

Retrospective quantitative detection of SARS-CoV-2 by digital PCR showing high accuracy for low viral load specimens

open access: yesJournal of Infection in Developing Countries, 2022
Introduction: Accurate detection of severe acute respiratory syndrome coronavirus 2 is critical for diagnosis and disease status evaluation of Coronavirus disease 2019.
Renfei Lu   +6 more
doaj   +1 more source

Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription

open access: yesViruses, 2009
There is ample evidence that synthesis of HIV-1 proviral DNA from the viral RNA genome during reverse transcription requires host factors. However, only a few cellular proteins have been described in detail that affect reverse transcription and interact ...
David Harrich   +3 more
doaj   +1 more source

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