Results 21 to 30 of about 1,473,689 (294)
mBio, 2020 A defining activity of retroviruses is reverse transcription, the process by which the viral genomic RNA is converted into the double-stranded DNA required for virus replication.
Jordan Jennings +4 more
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HIV-1 Reverse Transcription [PDF]
, 2014 Reverse transcription is an obligatory step in retrovirus replication in the course of which the retroviral RNA/DNA-dependent DNA polymerase (RT) copies the single-stranded positive sense RNA genome to synthesize the double-stranded viral DNA. At the same time the RT-associated RNaseH activity degrades the genomic RNA template, which has just been ...
Cimarelli, Andrea, Darlix, Jean-Luc
openaire +3 more sources
HIV-1 Reverse Transcription [PDF]
Cold Spring Harbor Perspectives in Medicine, 2012 Reverse transcription and integration are the defining features of the Retroviridae; the common name "retrovirus" derives from the fact that these viruses use a virally encoded enzyme, reverse transcriptase (RT), to convert their RNA genomes into DNA. Reverse transcription is an essential step in retroviral replication.
Wei-Shau, Hu, Stephen H, Hughes
openaire +2 more sources
Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]
, 2016 Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude +5 more
core +2 more sources
DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA. [PDF]
, 2017 Diversity-generating retroelements (DGRs) are molecular evolution machines that facilitate microbial adaptation to environmental changes. Hypervariation occurs via a mutagenic retrotransposition process from a template repeat (TR) to a variable repeat ...
Guo, Huatao +6 more
core +3 more sources
Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription
Viruses, 2009 There is ample evidence that synthesis of HIV-1 proviral DNA from the viral RNA genome during reverse transcription requires host factors. However, only a few cellular proteins have been described in detail that affect reverse transcription and interact ...
David Harrich +3 more
doaj +1 more source
Retrovirology, 2011 Background The nucleocapsid (NC) protein of HIV-1 is critical for viral replication. Mutational analyses have demonstrated its involvement in viral assembly, genome packaging, budding, maturation, reverse transcription, and integration.
Gorelick Robert J +2 more
doaj +1 more source
Complete Genome Sequence of Diaphorina citri-associated C virus, a Novel Putative RNA Virus of the Asian Citrus Psyllid, Diaphorina citri. [PDF]
, 2016 We present here the complete nucleotide sequence and genome organization of a novel putative RNA virus identified in field populations of the Asian citrus psyllid, Diaphorina citri, through sequencing of the transcriptome followed by reverse ...
Falk, Bryce W +2 more
core +1 more source
BMJ Paediatrics Open, 2020 Background Several studies indicated that children seem to be less frequently infected with SARS-CoV-2 and are potentially less contagious than adults. To examine the spread of SARS-CoV-2, we combined both Reverse transcription-PCR testing and serology ...
Emmanuelle Varon +17 more
doaj +1 more source
Biomedicines, 2022 Rapid and accurate detection and identification of pathogens in clinical samples is essential for all infection diseases. However, in the case of epidemics, it plays a key role not only in the implementation of effective therapy but also in limiting the ...
Aleksandra Anna Zasada +12 more
doaj +1 more source