Results 21 to 30 of about 994,970 (256)

Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells

Retrovirology, 2007
We have shown previously that HIV actively and selectively packages the spliced HIV RNAs into progeny virions. In the present study, by using a RT-QPCR and QPCR strategies, we show that spliced viral RNAs are present in infectious particles and ...
Morichaud Zakia, Houzet Laurent, Mougel Marylène   +2 more
doaj   +1 more source

Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription

BioTechniques, 2012
Primer-independent cDNA synthesis during reverse transcription hinders quantitative analysis of bidirectional mRNA synthesis in eukaryotes as well as in cells infected with RNA viruses.
Lin Feng, Susanna Lintula, Tho Huu Ho, Maria Anastasina, Annukka Paju, Caj Haglund, Ulf-Håkan Stenman, Kristina Hotakainen, Arto Orpana, Denis Kainov, Jakob Stenman   +10 more
doaj   +1 more source

Augmentation of reverse transcription by integrase through an interaction with host factor, SIP1/Gemin2 Is critical for HIV-1 infection. [PDF]

PLoS ONE, 2009
There has been accumulating evidence for the involvement of retroviral integrase (IN) in the reverse transcription of viral RNA. We previously identified a host factor, survival motor neuron-interacting protein 1 (SIP1/Gemin2) that binds to human ...
Hironori Nishitsuji, Takaya Hayashi, Takuya Takahashi, Masashi Miyano, Mari Kannagi, Takao Masuda   +5 more
doaj   +1 more source

Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions

Viruses, 2021
The human immunodeficiency virus type 1 (HIV-1) capsid and its disassembly, or capsid uncoating, has remained an active area of study over the past several decades. Our understanding of the HIV-1 capsid as solely a protective shell has since shifted with
Zachary Ingram, Douglas K. Fischer, Zandrea Ambrose   +2 more
doaj   +1 more source

Host SAMHD1 protein restricts endogenous reverse transcription of HIV-1 in nondividing macrophages

Retrovirology, 2018
Background SAM domain and HD domain containing protein 1 (SAMHD1) is a host anti-HIV-1 restriction factor known to suppress viral reverse transcription in nondividing myeloid cells by its dNTP triphosphorylase activity that depletes cellular dNTPs ...
Bijan Mahboubi, Christina Gavegnano, Dong-Hyun Kim, Raymond F. Schinazi, Baek Kim   +4 more
doaj   +1 more source

The Role of Capsid in the Early Steps of HIV-1 Infection: New Insights into the Core of the Matter

Viruses, 2021
In recent years, major advances in research and experimental approaches have significantly increased our knowledge on the role of the HIV-1 capsid in the virus life cycle, from reverse transcription to integration and gene expression.
Nawal AlBurtamani, Alwin Paul, Ariberto Fassati   +2 more
doaj   +1 more source

Initiation of HIV Reverse Transcription: Is Enzyme Flipping Required?

Viruses, 2011
Liu and colleagues have recently studied dynamic changes in the orientation of HIV reverse transcriptase (RT) on its nucleic acid substrate during initiation of DNA synthesis.
Matthias Götte
doaj   +1 more source

Tutorial: Guidelines for Single-Cell RT-qPCR

Cells, 2021
Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha, Mikael Kubista, Lukas Valihrach   +2 more
doaj   +1 more source

HIV-1 Reverse Transcriptase Inhibition by Major Compounds in a Kenyan Multi-Herbal Composition (CareVid™): In Vitro and In Silico Contrast

Pharmaceuticals, 2021
CareVid is a multi-herbal product used in southwest Kenya as an immune booster and health tonic and has been anecdotally described as improving the condition of HIV-positive patients.
Winnie Rotich, Nicholas J. Sadgrove, Eduard Mas-Claret, Guillermo F. Padilla-González, Anastasia Guantai, Moses K. Langat   +5 more
doaj   +1 more source

When is it time for reverse transcription to start and go?

Retrovirology, 2009
Upon cell infection by a retrovirus, the viral DNA polymerase, called reverse transcriptase (RT), copies the genomic RNA to generate the proviral DNA flanked by two long terminal repeats (LTR).
Darlix Jean-Luc, Houzet Laurent, Mougel Marylène   +2 more
doaj   +1 more source