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HIV-1 Reverse Transcription [PDF]
, 2014 Reverse transcription is an obligatory step in retrovirus replication in the course of which the retroviral RNA/DNA-dependent DNA polymerase (RT) copies the single-stranded positive sense RNA genome to synthesize the double-stranded viral DNA. At the same time the RT-associated RNaseH activity degrades the genomic RNA template, which has just been ...
Cimarelli, Andrea, Darlix, Jean-Luc
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Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription
Viruses, 2009 There is ample evidence that synthesis of HIV-1 proviral DNA from the viral RNA genome during reverse transcription requires host factors. However, only a few cellular proteins have been described in detail that affect reverse transcription and interact ...
David Harrich +3 more
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Nucleic Acids Research, 1978 The 3' terminus of tRNA was enzymatically elongated by an oligo(A) tail. A fragment of DNA polymerase I (E. coli) was used in the presence of manganese to phase and synthesize a cleavable primer at the oligo(A)-tRNA template. When the threedimensional structure of oligo(A)-tRNA is being unfolded under conditions where the primer is still hybridized at ...
B, Wittig, S, Wittig
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Retrovirology, 2011 Background The nucleocapsid (NC) protein of HIV-1 is critical for viral replication. Mutational analyses have demonstrated its involvement in viral assembly, genome packaging, budding, maturation, reverse transcription, and integration.
Gorelick Robert J +2 more
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HIV-1 Reverse Transcription [PDF]
Cold Spring Harbor Perspectives in Medicine, 2012 Reverse transcription and integration are the defining features of the Retroviridae; the common name "retrovirus" derives from the fact that these viruses use a virally encoded enzyme, reverse transcriptase (RT), to convert their RNA genomes into DNA. Reverse transcription is an essential step in retroviral replication.
Wei-Shau, Hu, Stephen H, Hughes
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BMJ Paediatrics Open, 2020 Background Several studies indicated that children seem to be less frequently infected with SARS-CoV-2 and are potentially less contagious than adults. To examine the spread of SARS-CoV-2, we combined both Reverse transcription-PCR testing and serology ...
Emmanuelle Varon +17 more
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Biomedicines, 2022 Rapid and accurate detection and identification of pathogens in clinical samples is essential for all infection diseases. However, in the case of epidemics, it plays a key role not only in the implementation of effective therapy but also in limiting the ...
Aleksandra Anna Zasada +12 more
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Thermal Effects on Reverse Transcription: Improvement of Accuracy and Processivity in cDNA Synthesis
BioTechniques, 2001 Reverse transcription, coupled with DNA amplification, has been widely used for molecular analysis of RNAs. Reverse transcriptases are retroviral DNA polymerases that can synthesize DNA from both RNA and DNA.
Christine M. Malboeuf +3 more
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BioTechniques, 2007 Over the past few years high-throughput platforms for real-time quantitative PCR have become widely available. The cost of RNA extraction from a large number of samples are, however, quite notable.
Ricarda Jost +2 more
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Primer tRNAs for reverse transcription [PDF]
Journal of Virology, 1997 Both retroviruses and long terminal repeat (LTR) retrotransposons use cellular tRNAs as primers for reverse transcription during their replication cycles. In retroviruses, primer tRNA is selectively packaged into the virion, where it is placed onto the primer binding site (PBS) of the viral RNA genome and used to prime the reverse transcriptase (RT ...
J, Mak, L, Kleiman
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