Results 31 to 40 of about 1,473,689 (294)

Thermal Effects on Reverse Transcription: Improvement of Accuracy and Processivity in cDNA Synthesis

BioTechniques, 2001
Reverse transcription, coupled with DNA amplification, has been widely used for molecular analysis of RNAs. Reverse transcriptases are retroviral DNA polymerases that can synthesize DNA from both RNA and DNA.
Christine M. Malboeuf, Seth J. Isaacs, Nancy H. Tran, Baek Kim   +3 more
doaj   +1 more source

Magnetic quantitative reverse transcription PCR: A high-throughput method for mRNA extraction and quantitative reverse transcription PCR

BioTechniques, 2007
Over the past few years high-throughput platforms for real-time quantitative PCR have become widely available. The cost of RNA extraction from a large number of samples are, however, quite notable.
Ricarda Jost, Oliver Berkowitz, Josette Masle   +2 more
doaj   +1 more source

Allosteric HIV-1 integrase inhibitors lead to premature degradation of the viral RNA genome and integrase in target cells [PDF]

, 2017
Recent evidence indicates that inhibition of HIV-1 integrase (IN) binding to the viral RNA genome by allosteric integrase inhibitors (ALLINIs) or through mutations within IN yields aberrant particles in which the viral ribonucleoprotein complexes (vRNPs)
Elliott, Jennifer, Errando, Manel, Koneru, Pratibha C, Kutluay, Sebla B, Kvaratskhelia, Mamuka, Lawson, Dana Q, Madison, Michaela K, Ozanturk, Ayse Naz, Townsend, Dana   +8 more
core   +2 more sources

Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells

Retrovirology, 2007
We have shown previously that HIV actively and selectively packages the spliced HIV RNAs into progeny virions. In the present study, by using a RT-QPCR and QPCR strategies, we show that spliced viral RNAs are present in infectious particles and ...
Morichaud Zakia, Houzet Laurent, Mougel Marylène   +2 more
doaj   +1 more source

Augmentation of reverse transcription by integrase through an interaction with host factor, SIP1/Gemin2 Is critical for HIV-1 infection. [PDF]

PLoS ONE, 2009
There has been accumulating evidence for the involvement of retroviral integrase (IN) in the reverse transcription of viral RNA. We previously identified a host factor, survival motor neuron-interacting protein 1 (SIP1/Gemin2) that binds to human ...
Hironori Nishitsuji, Takaya Hayashi, Takuya Takahashi, Masashi Miyano, Mari Kannagi, Takao Masuda   +5 more
doaj   +1 more source

Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions

Viruses, 2021
The human immunodeficiency virus type 1 (HIV-1) capsid and its disassembly, or capsid uncoating, has remained an active area of study over the past several decades. Our understanding of the HIV-1 capsid as solely a protective shell has since shifted with
Zachary Ingram, Douglas K. Fischer, Zandrea Ambrose   +2 more
doaj   +1 more source

Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]

, 2012
Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Van Peer, Gert, Mestdagh, Pieter, Vandesompele, Jo   +2 more
core   +1 more source

Host SAMHD1 protein restricts endogenous reverse transcription of HIV-1 in nondividing macrophages

Retrovirology, 2018
Background SAM domain and HD domain containing protein 1 (SAMHD1) is a host anti-HIV-1 restriction factor known to suppress viral reverse transcription in nondividing myeloid cells by its dNTP triphosphorylase activity that depletes cellular dNTPs ...
Bijan Mahboubi, Christina Gavegnano, Dong-Hyun Kim, Raymond F. Schinazi, Baek Kim   +4 more
doaj   +1 more source

Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer-Evaluation of Several Markers with Real-Time Reverse Transcription-PCR [PDF]

, 2013
It is widely known that cells from epithelial tumors, e. g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic ...
Alexandra Kölbl, Alix-Panabieres, Balbin, Bergqvist, Bernard, Bolke, Braun, Braun, Chang, Christian Schindlbeck, De Cremoux, Dontu, Dydensborg, Fabre-Lafay, Fleming, Frasor, Gervasoni, Giani, Guo, Hedenfalk, Inaba, Jiang, Julia Neugebauer, Kim, Klaus Friese, Konecny, Krawczyk, Kurec, Lafleur, Livak, Markou, Matthias Ilmer, Mendoza, Mitas, Moliterni, Nikseresht, Noack, Pan, Pantel, Ring, Selvamurugan, Simone Hofmann, Slamon, Smerage, Solomayer, Stefan Hutter, Tai, Udo Jeschke, Ulrich Andergassen, Vandesompele, Verena Engelstädter, Wang, Weissenstein, Wu, Wu, Zhang   +55 more
core   +3 more sources

Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription

BioTechniques, 2012
Primer-independent cDNA synthesis during reverse transcription hinders quantitative analysis of bidirectional mRNA synthesis in eukaryotes as well as in cells infected with RNA viruses.
Lin Feng, Susanna Lintula, Tho Huu Ho, Maria Anastasina, Annukka Paju, Caj Haglund, Ulf-Håkan Stenman, Kristina Hotakainen, Arto Orpana, Denis Kainov, Jakob Stenman   +10 more
doaj   +1 more source