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Reverse Transcription–Polymerase Chain Reaction
Cold Spring Harbor Protocols, 2014Reverse transcription coupled to the polymerase chain reaction (RT–PCR) is commonly used to detect the presence of mRNAs, pre-mRNAs, or other types of RNA such as noncoding RNAs. The method involves using a primer annealed to the RNA of interest. For mRNA, the primer is usually a synthetic oligo(dT)15–18, a random hexamer mixture (dN)6, or a synthetic ...
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Reverse Engineering Transcriptional Gene Networks
2013The aim of this chapter is a step-by-step guide on how to infer gene networks from gene expression profiles. The definition of a gene network is given in Subheading 1, where the different types of networks are discussed. The chapter then guides the readers through a data-gathering process in order to build a compendium of gene expression profiles from ...
Belcastro V, DI BERNARDO, DIEGO
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Reverse-Transcription PCR (RT-PCR)
2013RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription
Science, 2023Max E Wilkinson +2 more
exaly
Retroviral reverse transcription
Virus Research, 2008Luis Menéndez-Arias, Ben Berkhout
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