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Sensitivity of Reverse Transcription Polymerase Chain Reaction Tests for Severe Acute Respiratory Syndrome Coronavirus 2 Through Time. [PDF]

J Infect Dis, 2022
Background Reverse transcription polymerase chain reaction (RT-PCR) tests are the gold standard for detecting recent infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Reverse transcription PCR sensitivity varies over the course
Binny RN, Priest P, French NP, Parry M, Lustig A, Hendy SC, Maclaren OJ, Ridings KM, Steyn N, Vattiato G, Plank MJ.   +10 more
europepmc   +2 more sources

Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles [PDF]

Scientific Reports, 2018
AbstractWe assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR). We prepared GUVs that encapsulated one-pot RT-PCR reaction mixture including template RNA, primers, and Taqman probe, using water-in-oil emulsion transfer method.
Mamiko Tsugane, Hiroaki Suzuki
openaire   +3 more sources

Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction [PDF]

Memorias do Instituto Oswaldo Cruz, 2005
We describe a reverse transcription-polymerase chain reaction (RT-PCR) and a nested-PCR for diagnosis of Piry, Carajás, Cocal, and Alagoas vesiculoviruses from Brazil.
Daniela Wey Bonutti, Luiz Tadeu Moraes Figueiredo   +1 more
doaj   +4 more sources

Comparative evaluation of truenat reverse transcription-polymerase chain reaction with commercially available reverse transcription-polymerase chain reaction kits for COVID-19 diagnosis

Advances in Human Biology, 2022
Introduction: Early and correct identification of the betacoronavirus is important for effective isolation treatment and case management. Real-time polymerase chain reaction (PCR) are consider as a gold standard for the diagnosis of severe acute ...
Anoop Jaiswal, Monica Singh, Arindam Chakraborty   +2 more
doaj   +2 more sources

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Emerging Infectious Diseases, 2004
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV).
Shannon L. Emery, Dean D. Erdman, Michael D. Bowen, Bruce R. Newton, Jonas M. Winchell, Richard F. Meyer, Suxiang Tong, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Paul A. Rota, Bettina Bankamp, Luis E. Lowe, Tom G. Ksiazek, William J. Bellini, Larry J. Anderson   +15 more
doaj   +2 more sources

Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived Taq polymerase [PDF]

Annals of Thoracic Medicine
BACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse
Mahmoud Zhra, Aljohara Al Saud, Maha Alzayer, Liliane Okdah, Hani Tamim, Hana M. A Fakhoury, Ahmad Aljada   +6 more
doaj   +2 more sources

An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 [PDF]

Frontiers in Microbiology, 2021
We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and ...
Jadranka Milosevic, Jadranka Milosevic, Mengrou Lu, Wallace Greene, Hong-Zhang He, Hong-Zhang He, Si-Yang Zheng, Si-Yang Zheng   +7 more
doaj   +2 more sources

Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection. [PDF]

PLoS Neglected Tropical Diseases, 2016
The definitive diagnosis of lyssavirus infection (including rabies) in animals and humans is based on laboratory confirmation. The reference techniques for post-mortem rabies diagnosis are still based on direct immunofluorescence and virus isolation, but
Laurent Dacheux, Florence Larrous, Rachel Lavenir, Anthony Lepelletier, Abdellah Faouzi, Cécile Troupin, Jalal Nourlil, Philippe Buchy, Herve Bourhy   +8 more
doaj   +2 more sources

Detection of Feline Coronavirus Membrane Gene Based on Conventional Revere Transcription-Polymerase Chain Reaction, Nested Reverse Transcription-Polymerase Chain Reaction, and Reverse Transcription-Quantitative Polymerase Chain Reaction: A Comparative Study. [PDF]

Int J Mol Sci
Feline coronavirus (FCoV) is a major pathogen causing feline infectious peritonitis (FIP), a lethal disease in cats, necessitating accurate diagnostic methods. This study developed and compared novel primers targeting the FCoV membrane (M) gene for enhanced detection.
Kopduang C, Rapichai W, Leangcharoenpong C, Khamsingnok P, Puangmalee T, Ratanabunyong S, Rattanasrisomporn A, Khaoiam T, Dong HV, Choowongkomol K, Rattanasrisomporn J.   +10 more
europepmc   +2 more sources

Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection. [PDF]

Food Sci Biotechnol
Aichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with non-bacterial gastroenteritis in Aichi Prefecture, Japan, in 1989. AiV has been reported from in various aquatic environments, such as surface water and sewage, can be transmitted via the fecal-oral route through ...
Kim MG, Yoo B, Min AY, Seo DW, Choi C, Kim SH, Kim SH.   +6 more
europepmc   +3 more sources