Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction [PDF]
Memorias do Instituto Oswaldo Cruz, 2005 We describe a reverse transcription-polymerase chain reaction (RT-PCR) and a nested-PCR for diagnosis of Piry, Carajás, Cocal, and Alagoas vesiculoviruses from Brazil.
Daniela Wey Bonutti +1 more
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A new reverse transcription-polymerase chain reaction method for accurate quantification [PDF]
BMC Biotechnology, 2003 Background Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the
Shiao Yih-Horng
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Advances in Human Biology, 2022 Introduction: Early and correct identification of the betacoronavirus is important for effective isolation treatment and case management. Real-time polymerase chain reaction (PCR) are consider as a gold standard for the diagnosis of severe acute ...
Anoop Jaiswal +2 more
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t(15;17) Reverse Transcription-Polymerase Chain Reaction with Alternative Splicing [PDF]
The Journal of Molecular Diagnostics, 2001 The Journal of Molecular Diagnostics would like to announce the establishment of a new feature in the Journal , “Consultations in Molecular Diagnostics.” This manuscript category will include short , case-directed articles meant to illustrate a classic principle , a difficult interpretation , or a new approach or application in molecular diagnostics ...
Raj K. Rolston, Jeffrey A. Kant
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Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Archives of Razi Institute, 2020 Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
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Reverse Transcription Polymerase Chain Reaction Pattern of SARS-CoV-2 Beta Variant
Acta Médica Portuguesa, 2021 N/a.
Daniela Fonseca e Silva +2 more
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Automated Reverse Transcription Polymerase Chain Reaction Data Analysis for Sars-CoV-2 Detection
Revista de Investigación Clínica, 2021 Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is a current public health concern. Rapid diagnosis is crucial, and reverse transcription polymerase chain reaction (RT-PCR) is presently the reference standard for ...
Laura Gómez-Romero +4 more
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Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles [PDF]
Scientific Reports, 2018 AbstractWe assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR). We prepared GUVs that encapsulated one-pot RT-PCR reaction mixture including template RNA, primers, and Taqman probe, using water-in-oil emulsion transfer method.
Hiroaki Suzuki +2 more
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SAGE Open Medicine, 2022 Objective: Reverse transcription-polymerase chain reaction is a gold standard diagnostic tool for coronavirus disease-2019. Limited coverage and long turnaround times are linked to the poor response to the pandemic in developing countries like Ethiopia ...
Dejene Dessalegn +3 more
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Identification of reference genes for relative quantification of circulating microRNAs in bovine serum. [PDF]
PLoS ONE, 2015 Circulating microRNAs in body fluids have been implicated as promising biomarkers for physiopathology disorders. Currently, the expression levels of circulating microRNAs are estimated by reverse transcription quantitative real-time polymerase chain ...
In-Seon Bae +7 more
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