Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction [PDF]
Memorias do Instituto Oswaldo Cruz, 2005 We describe a reverse transcription-polymerase chain reaction (RT-PCR) and a nested-PCR for diagnosis of Piry, Carajás, Cocal, and Alagoas vesiculoviruses from Brazil.
Daniela Wey Bonutti +1 more
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A new reverse transcription-polymerase chain reaction method for accurate quantification [PDF]
BMC Biotechnology, 2003 Background Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the
Shiao Yih-Horng
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Advances in Human Biology, 2022 Introduction: Early and correct identification of the betacoronavirus is important for effective isolation treatment and case management. Real-time polymerase chain reaction (PCR) are consider as a gold standard for the diagnosis of severe acute ...
Anoop Jaiswal +2 more
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Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived Taq polymerase [PDF]
Annals of Thoracic MedicineBACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse
Mahmoud Zhra +6 more
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An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 [PDF]
Frontiers in Microbiology, 2021 We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and ...
Jadranka Milosevic +7 more
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t(15;17) Reverse Transcription-Polymerase Chain Reaction with Alternative Splicing [PDF]
The Journal of Molecular Diagnostics, 2001 The Journal of Molecular Diagnostics would like to announce the establishment of a new feature in the Journal , “Consultations in Molecular Diagnostics.” This manuscript category will include short , case-directed articles meant to illustrate a classic principle , a difficult interpretation , or a new approach or application in molecular diagnostics ...
Raj K. Rolston, Jeffrey A. Kant
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Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection. [PDF]
Food Sci BiotechnolAichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with non-bacterial gastroenteritis in Aichi Prefecture, Japan, in 1989. AiV has been reported from in various aquatic environments, such as surface water and sewage, can be transmitted via the fecal-oral route through ...
Kim MG +6 more
europepmc +3 more sources
Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Archives of Razi Institute, 2020 Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
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Reverse Transcription Polymerase Chain Reaction Pattern of SARS-CoV-2 Beta Variant
Acta Médica Portuguesa, 2021 N/a.
Daniela Fonseca e Silva +2 more
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Automated Reverse Transcription Polymerase Chain Reaction Data Analysis for Sars-CoV-2 Detection
Revista de Investigación Clínica, 2021 Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is a current public health concern. Rapid diagnosis is crucial, and reverse transcription polymerase chain reaction (RT-PCR) is presently the reference standard for ...
Laura Gómez-Romero +4 more
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