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Sensitivity of Reverse Transcription Polymerase Chain Reaction Tests for Severe Acute Respiratory Syndrome Coronavirus 2 Through Time. [PDF]

J Infect Dis, 2022
Background Reverse transcription polymerase chain reaction (RT-PCR) tests are the gold standard for detecting recent infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Reverse transcription PCR sensitivity varies over the course
Binny RN, Priest P, French NP, Parry M, Lustig A, Hendy SC, Maclaren OJ, Ridings KM, Steyn N, Vattiato G, Plank MJ.   +10 more
europepmc   +2 more sources

Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles [PDF]

Scientific Reports, 2018
We assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR).
M. Tsugane, Hiroaki Suzuki
semanticscholar   +3 more sources

Computed Tomographic Imaging of 3 Patients With Coronavirus Disease 2019 Pneumonia With Negative Virus Real-time Reverse-Transcription Polymerase Chain Reaction Test [PDF]

Clinical Infectious Diseases, 2020
We reported computed tomographic (CT) imaging findings of 3 patients with coronavirus disease 2019 (COVID-19) pneumonia with initially negative results before CT examination and finally confirmed positive for the severe acute respiratory syndrome ...
Junqing Xu, Ruodai Wu, Hua Huang, Weidong Zheng, Xinling Ren, Nashan Wu, Bin Ji, Yungang Lv, Yumeng Liu, Rui Mi   +9 more
openalex   +2 more sources

Prolonged existence of SARS-CoV-2 RNAs in the extracellular vesicles of respiratory specimens from patients with negative reverse transcription-polymerase chain reaction. [PDF]

Liver Res, 2023
Subudhi PD, Rooge S, Bihari C, Thangariyal S, Goswami S, Agarwal R, Kaur S, Gupta E, Baweja S.   +8 more
europepmc   +2 more sources

Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection. [PDF]

Food Sci Biotechnol
Aichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with non-bacterial gastroenteritis in Aichi Prefecture, Japan, in 1989. AiV has been reported from in various aquatic environments, such as surface water and sewage, can be transmitted via the fecal-oral route through ...
Kim MG, Yoo B, Min AY, Seo DW, Choi C, Kim SH, Kim SH.   +6 more
europepmc   +3 more sources

Detection of Feline Coronavirus Membrane Gene Based on Conventional Revere Transcription-Polymerase Chain Reaction, Nested Reverse Transcription-Polymerase Chain Reaction, and Reverse Transcription-Quantitative Polymerase Chain Reaction: A Comparative Study. [PDF]

Int J Mol Sci
Feline coronavirus (FCoV) is a major pathogen causing feline infectious peritonitis (FIP), a lethal disease in cats, necessitating accurate diagnostic methods. This study developed and compared novel primers targeting the FCoV membrane (M) gene for enhanced detection.
Kopduang C, Rapichai W, Leangcharoenpong C, Khamsingnok P, Puangmalee T, Ratanabunyong S, Rattanasrisomporn A, Khaoiam T, Dong HV, Choowongkomol K, Rattanasrisomporn J.   +10 more
europepmc   +2 more sources

Occurrence and Timing of Subsequent Severe Acute Respiratory Syndrome Coronavirus 2 Reverse-transcription Polymerase Chain Reaction Positivity Among Initially Negative Patients

Clinical Infectious Diseases, 2020
Using data for 20 912 patients from 2 large academic health systems, we analyzed the frequency of severe acute respiratory syndrome coronavirus 2 reverse-transcription polymerase chain reaction test discordance among individuals initially testing ...
Dustin R. Long, S. Gombar, C. Hogan, Alexander L Greninger, Vikas O’Reilly-Shah, C. Bryson-Cahn, Bryan A. Stevens, A. Rustagi, K. Jerome, C. Kong, J. Zehnder, N. Shah, N. Weiss, B. Pinsky, Jacob E. Sunshine   +14 more
semanticscholar   +1 more source

Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction [PDF]

Memórias do Instituto Oswaldo Cruz, 2005
We describe a reverse transcription-polymerase chain reaction (RT-PCR) and a nested-PCR for diagnosis of Piry, Carajás, Cocal, and Alagoas vesiculoviruses from Brazil. The RNA extracts of viral and clinical samples were submitted to a RT-PCR using Vesiculovirus G primers that amplify part of the glycoprotein gene.
Daniela Wey Bonutti, Luiz Tadeu Moraes Figueiredo   +1 more
openaire   +4 more sources

Reverse transcription and polymerase chain reaction: principles and applications in dentistry [PDF]

Journal of Applied Oral Science, 2004
Various molecular biology techniques have become available in the last few years. One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in 1985. This method relies on the exponential amplification of specific DNA fragments, resulting in millions of copies that
Santos, Carlos Ferreira dos, Sakai, Vivien Thiemy, Machado, Maria Aparecida de Andrade Moreira, Schippers, Daniela Nicole, Greene, Andrew Seth   +4 more
openaire   +4 more sources

t(15;17) Reverse Transcription-Polymerase Chain Reaction with Alternative Splicing [PDF]

The Journal of Molecular Diagnostics, 2001
The Journal of Molecular Diagnostics would like to announce the establishment of a new feature in the Journal , “Consultations in Molecular Diagnostics.” This manuscript category will include short , case-directed articles meant to illustrate a classic principle , a difficult interpretation , or a new approach or application in molecular diagnostics ...
R K, Rolston, J A, Kant
openaire   +2 more sources