Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction [PDF]
Memorias do Instituto Oswaldo Cruz, 2005 We describe a reverse transcription-polymerase chain reaction (RT-PCR) and a nested-PCR for diagnosis of Piry, Carajás, Cocal, and Alagoas vesiculoviruses from Brazil.
Daniela Wey Bonutti +1 more
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Advances in Human Biology, 2022 Introduction: Early and correct identification of the betacoronavirus is important for effective isolation treatment and case management. Real-time polymerase chain reaction (PCR) are consider as a gold standard for the diagnosis of severe acute ...
Anoop Jaiswal +2 more
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Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived Taq polymerase [PDF]
Annals of Thoracic MedicineBACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse
Mahmoud Zhra +6 more
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An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 [PDF]
Frontiers in Microbiology, 2021 We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and ...
Jadranka Milosevic +7 more
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Prolonged existence of SARS-CoV-2 RNAs in the extracellular vesicles of respiratory specimens from patients with negative reverse transcription-polymerase chain reaction. [PDF]
Liver Res, 2023 Subudhi PD +8 more
europepmc +2 more sources
Detection of Feline Coronavirus Membrane Gene Based on Conventional Revere Transcription-Polymerase Chain Reaction, Nested Reverse Transcription-Polymerase Chain Reaction, and Reverse Transcription-Quantitative Polymerase Chain Reaction: A Comparative Study. [PDF]
Int J Mol SciFeline coronavirus (FCoV) is a major pathogen causing feline infectious peritonitis (FIP), a lethal disease in cats, necessitating accurate diagnostic methods. This study developed and compared novel primers targeting the FCoV membrane (M) gene for enhanced detection.
Kopduang C +10 more
europepmc +2 more sources
Frontiers in Oncology, 2022 BackgroundThe role of circular RNAs (circRNAs) in the occurrence of gastric cancer is still unclear. Therefore, the diagnostic value and mechanisms underlying hsa_circ_0061276 in the occurrence of gastric cancer were explored.MethodsReverse transcription-
Yao Ruan +8 more
doaj +2 more sources
Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay. [PDF]
Poult Sci, 2023 Piri-Gharaghie T +6 more
europepmc +2 more sources
Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection. [PDF]
Food Sci BiotechnolAichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with non-bacterial gastroenteritis in Aichi Prefecture, Japan, in 1989. AiV has been reported from in various aquatic environments, such as surface water and sewage, can be transmitted via the fecal-oral route through ...
Kim MG +6 more
europepmc +3 more sources
Jurnal Veteriner, 2015 Avian influenza virus subtype H5N1 (AIV H5N1) is highly pathogenic and fatal in poultry. The virusis still endemic with low virulence rate, although it may play a critical role in causing high morbidity andmortality rates in poultry in Indonesia.
Raden Wasito +4 more
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