Prolonged existence of SARS-CoV-2 RNAs in the extracellular vesicles of respiratory specimens from patients with negative reverse transcription-polymerase chain reaction. [PDF]
Subudhi PD+8 more
europepmc +1 more source
P450 Aromatase Messenger Ribonucleic Acid Expression in Male Rat Germ Cells: Detection by Reverse Transcription‐Polymerase Chain Reaction Amplification [PDF]
Lynn Janulis+3 more
openalex +1 more source
Membrane fusion‐inspired nanomaterials offer transformative potential in diagnostics by mimicking natural fusion processes to achieve highly sensitive and specific detection of disease biomarkers. This review highlights recent advancements in nanomaterial functionalization strategies, signal amplification systems, and stimuli‐responsive fusion designs,
Sojeong Lee+9 more
wiley +1 more source
Persistent Positive Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR) Results in Recovered COVID-19 Hospital Patients: Implications for Interpretation. [PDF]
Hur J, Kim DM, Park D, Chang MC.
europepmc +1 more source
Nanobody‐Decorated Lipid Nanoparticles for Enhanced mRNA Delivery to Tumors In Vivo
This study presents a targeted mRNA‐LNP platform engineered with anti‐PSMA nanobodies for prostate cancer therapy. Using different in vivo models, the authors demonstrate selective uptake and mRNA delivery in PSMA+ cancer cells. However, while targeting is enhanced, mRNA delivery efficacy is limited by tissue penetration, tumor necrosis, and immune ...
Pol Escudé Martinez de Castilla+16 more
wiley +1 more source
Evaluation of reverse transcription-polymerase chain reaction and simultaneous amplification and testing for quantitative detection of serum hepatitis B virus RNA. [PDF]
Hu X+8 more
europepmc +1 more source
Analysis of the t(2;5)(p23;q35) translocation by reverse transcription- polymerase chain reaction in CD30+ anaplastic large-cell lymphomas, in other non-Hodgkin's lymphomas of T-cell phenotype, and in Hodgkin's disease [PDF]
Axel Wellmann+5 more
openalex +1 more source
Bone fracture healing is a complex, timely orchestrated scenario involving multiple cell types and a plethora of cytokines and regulatory factors. To gain further insight, an in vitro model to a) study macrophage polarization under mechanical load in a fibrin hydrogel and b) subsequently assess the effect of conditioned media derived from macrophages ...
Anne Géraldine Guex+4 more
wiley +1 more source
Impact of Nanoparticle Stiffness on Endosomal Escape and Signaling Pathways in Cytosolic Delivery
Nanoparticle (NP) stiffness affects cellular uptake, but its impact on intracellular distribution remains unclear. This study synthesizes silica nanocapsules with varying stiffness, inspired by viral mechanisms, and applies assays to measure cellular uptake and escape efficiency.
Yali Zhang+6 more
wiley +1 more source
Monitoring of Newcastle Disease Virus Vaccine Strain Replication in Embryonated Chicken Eggs by Reverse Transcription-Polymerase Chain Reaction. [PDF]
Ghadimipour R+5 more
europepmc +1 more source