Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
doaj +1 more source
Diagnostic Efficacy of RealStar SARS-CoV-2 Reverse Transcription-Polymerase Chain Reaction (RT-PCR) in Comparison to GeneXpert System for the Detection of COVID-19. [PDF]
Jawdat DM +8 more
europepmc +3 more sources
Reverse Transcription Polymerase Chain Reaction Pattern of SARS-CoV-2 Beta Variant
N/a.
Daniela Fonseca e Silva +2 more
doaj +1 more source
Automated Reverse Transcription Polymerase Chain Reaction Data Analysis for Sars-CoV-2 Detection
Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is a current public health concern. Rapid diagnosis is crucial, and reverse transcription polymerase chain reaction (RT-PCR) is presently the reference standard for ...
Laura Gómez-Romero +4 more
doaj +1 more source
Objective: Reverse transcription-polymerase chain reaction is a gold standard diagnostic tool for coronavirus disease-2019. Limited coverage and long turnaround times are linked to the poor response to the pandemic in developing countries like Ethiopia ...
Dejene Dessalegn +3 more
doaj +1 more source
Identification of reference genes for relative quantification of circulating microRNAs in bovine serum. [PDF]
Circulating microRNAs in body fluids have been implicated as promising biomarkers for physiopathology disorders. Currently, the expression levels of circulating microRNAs are estimated by reverse transcription quantitative real-time polymerase chain ...
In-Seon Bae +7 more
doaj +1 more source
Recent viral pathogen in acute gastroenteritis: a retrospective study at a tertiary hospital for 1 year [PDF]
PurposeViral gastroenteritis among children is mainly caused by rotavirus, norovirus, astrovirus, or adenovirus strains. However, changing socioeconomic conditions and a rotavirus vaccination program may be affecting the prevalence of these viral ...
Hye Il Jin +3 more
doaj +1 more source
Introduction Diagnostic procedure in Coronavirus Disease 2019 (COVID-19) is based mainly on performing real-time-reverse transcription-polymerase chain-reaction (RT-PCR), which has been accepted as the gold standard method.
Anna Dworzańska +4 more
doaj +1 more source
Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles [PDF]
AbstractWe assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR). We prepared GUVs that encapsulated one-pot RT-PCR reaction mixture including template RNA, primers, and Taqman probe, using water-in-oil emulsion transfer method.
Mamiko Tsugane, Hiroaki Suzuki
openaire +2 more sources
Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV).
Shannon L. Emery +15 more
doaj +1 more source

