Results 11 to 20 of about 329,236 (311)
Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles [PDF]
Scientific Reports, 2018 AbstractWe assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR). We prepared GUVs that encapsulated one-pot RT-PCR reaction mixture including template RNA, primers, and Taqman probe, using water-in-oil emulsion transfer method.
Hiroaki Suzuki +2 more
openaire +2 more sources
SAGE Open Medicine, 2022 Objective: Reverse transcription-polymerase chain reaction is a gold standard diagnostic tool for coronavirus disease-2019. Limited coverage and long turnaround times are linked to the poor response to the pandemic in developing countries like Ethiopia ...
Dejene Dessalegn +3 more
doaj +1 more source
Identification of reference genes for relative quantification of circulating microRNAs in bovine serum. [PDF]
PLoS ONE, 2015 Circulating microRNAs in body fluids have been implicated as promising biomarkers for physiopathology disorders. Currently, the expression levels of circulating microRNAs are estimated by reverse transcription quantitative real-time polymerase chain ...
In-Seon Bae +7 more
doaj +1 more source
Recent viral pathogen in acute gastroenteritis: a retrospective study at a tertiary hospital for 1 year [PDF]
Korean Journal of Pediatrics, 2016 PurposeViral gastroenteritis among children is mainly caused by rotavirus, norovirus, astrovirus, or adenovirus strains. However, changing socioeconomic conditions and a rotavirus vaccination program may be affecting the prevalence of these viral ...
Hye Il Jin +3 more
doaj +1 more source
The comparison of chest CT and RT-PCR during the diagnosis of COVID-19 [PDF]
Ķazaķstannyṇ Klinikalyķ Medicinasy, 2021 Objective: Our aim in this study is to compare the diagnostic accuracy of chest computed tomography and Real-time Reverse Transcriptase Polymerase Chain Reaction in a population with suspected Coronavirus Disease 2019 (COVID-19) presenting at the ...
Avni Uygar Seyhan +5 more
doaj +1 more source
Annals of Agricultural and Environmental Medicine, 2020 Introduction Diagnostic procedure in Coronavirus Disease 2019 (COVID-19) is based mainly on performing real-time-reverse transcription-polymerase chain-reaction (RT-PCR), which has been accepted as the gold standard method.
Anna Dworzańska +4 more
doaj +1 more source
Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
Emerging Infectious Diseases, 2004 A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV).
Shannon L. Emery +15 more
doaj +1 more source
Reverse-Transcription Polymerase Chain Reaction Detection of the Enteroviruses [PDF]
Archives of Pathology & Laboratory Medicine, 1999 AbstractObjective.—This review focuses on commercial and in-house–developed reverse-transcription polymerase chain reaction (RT-PCR) assays used for the detection of enteroviral infections. In addition to providing details on the performance of RT-PCR, its specificity, and sensitivity, the clinical utility of this diagnostic method with specific ...
openaire +3 more sources
PLoS ONE, 2022 Rapid screening and diagnosis of coronavirus disease 2019 in the emergency department is important for controlling infections. When polymerase chain reaction tests cannot be rapidly performed, rapid antigen testing is often used, albeit with insufficient
Kosuke Mori +5 more
doaj
Journal of Medical Case Reports, 2020 Background Determining the infectiousness of patients with coronavirus disease 2019 is crucial for patient management. Medical staff usually refer to the results of reverse transcription polymerase chain reaction tests in conjunction with clinical ...
Masafumi Kanamoto +3 more
doaj +1 more source