Influenza A H5N1 Detection [PDF]
Emerging Infectious Diseases, 2005 We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples.
Enders K.O. Ng +4 more
doaj +6 more sources
Paradigm shift in the diagnosis of peste des petits ruminants: scoping review [PDF]
Acta Veterinaria Scandinavica, 2020 Peste des petits ruminants virus causes a highly contagious disease, which poses enormous economic losses in domestic animals and threatens the conservation of wild herbivores.
Edson Kinimi +4 more
doaj +2 more sources
Dengue-1 Virus Isolation during First Dengue Fever Outbreak on Easter Island, Chile [PDF]
Emerging Infectious Diseases, 2003 Dengue virus was detected for the first time in Chile, in an outbreak of dengue fever on Easter Island. The virus was isolated in tissue culture and characterized by reverse transcription–polymerase chain reaction as being dengue type 1.
Cecilia Perret +7 more
doaj +3 more sources
Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived Taq polymerase [PDF]
Annals of Thoracic MedicineBACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse
Mahmoud Zhra +6 more
doaj +2 more sources
An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 [PDF]
Frontiers in Microbiology, 2021 We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and ...
Jadranka Milosevic +7 more
doaj +2 more sources
Travel-associated Rabies in Austrian Man [PDF]
Emerging Infectious Diseases, 2005 Rabies developed in an Austrian man after he was bitten by a dog in Agadir, Morocco. Diagnosis was confirmed by reverse transcription–polymerase chain reaction and immunohistochemistry. The patient's girlfriend was bitten by the same dog, but she did not
Robert Krause +8 more
doaj +3 more sources
Reverse transcription and polymerase chain reaction: principles and applications in dentistry [PDF]
Journal of Applied Oral Science, 2004 Various molecular biology techniques have become available in the last few years. One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in 1985. This method relies on the exponential amplification of specific DNA fragments, resulting in millions of copies that
Santos, Carlos Ferreira dos +4 more
openaire +8 more sources
Rapid West Nile Virus Antigen Detection [PDF]
Emerging Infectious Diseases, 2005 We compared the VecTest WNV antigen assay with standard methods of West Nile virus (WNV) detection in swabs from American Crows (Corvus brachyrhynchos) and House Sparrows (Passer domesticus). The VecTest detected WNV more frequently than the plaque assay
Nicholas A. Panella +7 more
doaj +3 more sources
Oropouche Virus Isolation, Southeast Brazil [PDF]
Emerging Infectious Diseases, 2005 An Oropouche virus strain was isolated from a novel host (Callithrix sp.) in Arinos, Minas Gerais State, southeastern Brazil. The virus was identified by complement fixation test and confirmed by reverse transcription–polymerase chain reaction ...
Márcio Roberto Teixeira Nunes +6 more
doaj +3 more sources
Results of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Examination in Covid-19 Patients at Prof. Dr. H. Aloei Saboe City of Gorontalo [PDF]
, 2023 Coronavirus Disease or COVID-19 is still a concern throughout the world. WHO recommends the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method as the gold standard for diagnosing COVID-19.
Adipu, Rahma Yulia +2 more
core +1 more source