Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. [PDF]
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2012 We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively.
V. Corman +16 more
semanticscholar +4 more sources
Biochemical and transcriptomic analyses reveal different metabolite biosynthesis profiles among three color and developmental stages in ‘Anji Baicha’ (Camellia sinensis) [PDF]
, 2016 Primers used for quantitative reverse transcription polymerase chain reaction analyses.
Chun-Fang Li +7 more
core +6 more sources
Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]
, 2012 Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Mestdagh, Pieter +2 more
core +2 more sources
Influenza a H5N1 detection [PDF]
, 2005 We developed a sensitive and rapid real-time reverse transcription- polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples.
Cheng, PKC +4 more
core +1 more source
Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation [PDF]
, 2015 The identification of chromosomal rearrangements is of utmost importance for the diagnosis and classification of specific leukaemia subtypes and therefore has an impact on therapy choices in individual cases.
Federico, C +4 more
core +2 more sources
Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]
, 2016 Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude +5 more
core +2 more sources
Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) [PDF]
, 2014 The suitability of nested reverse transcription polymerase chain reaction (nRT-PCR) to detect betanodavirus in blood samples from naturally infected Senegalese sole (Solea senegalensis) was evaluated in comparison with other diagnostic methods.
Bandín, I. +4 more
core +1 more source
The impact of co-infection of influenza A virus on the severity of Middle East Respiratory Syndrome Coronavirus [PDF]
, 2017 This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Al-Tawfiq, Jaffar A. +4 more
core +1 more source
Advances in Human Biology, 2022 Introduction: Early and correct identification of the betacoronavirus is important for effective isolation treatment and case management. Real-time polymerase chain reaction (PCR) are consider as a gold standard for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, for that, there are a ...
Anoop Jaiswal +2 more
openaire +2 more sources
Memórias do Instituto Oswaldo Cruz, 2017 We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT ...
F. Naveca +5 more
semanticscholar +1 more source