Results 21 to 30 of about 677,547 (352)

Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels in Nigeria, 2015 [PDF]

, 2015
Evidence of current and past Middle East respiratory syndrome coronavirus (MERS-CoV) infection in dromedary camels slaughtered at an abattoir in Kano, Nigeria in January 2015, was sought by reverse transcription-quantitative polymerase chain reaction (RT-
Chan, MS, Chu, KW, Kuranga, SA, Oladipo, JO, Peiris, JSM, Perera, RAPM, Poon, LLM   +6 more
core   +1 more source

Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma [PDF]

, 2015
Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy.
Asadi, J., Ghanbari, R., Knuutila, S., Malekzadeh, R., Mohamadkhani, A., Mosakhani, N., Mowla, S.J., Nouraee, N., Poustchi, H., Yazdani, Y.   +9 more
core   +1 more source

RT-PCR Analysis of TOPBP1 Gene Expression in Hereditary Breast Cancer [PDF]

, 2010
Hereditary predisposition to breast cancer determined in large part by loss of function mutations in one of two genes BRCA1 and BRCA2. Besides BRCA1 and BRCA2 other genes are also likely to be involved in hereditary predisposition to breast cancer ...
Bernaciak, Magdalena, Bryś, Magdalena, Forma, Ewa, Romanowicz-Makowska, Hanna   +3 more
core   +1 more source

Evaluation of RealStar Reverse Transcription–Polymerase Chain Reaction Kits for Filovirus Detection in the Laboratory and Field

Journal of Infectious Diseases, 2016
Background. Diagnosis of Ebola virus (EBOV) disease (EVD) requires laboratory testing. Methods. The RealStar Filovirus Screen reverse transcription–polymerase chain reaction (RT-PCR) kit and the derived RealStar Zaire Ebolavirus RT-PCR kit were validated
Toni Rieger, Romy Kerber, Hussein El Halas, Elisa Pallasch, S. Duraffour, S. Günther, Stephan Ölschläger   +6 more
semanticscholar   +1 more source

Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection

PLoS Neglected Tropical Diseases, 2016
The definitive diagnosis of lyssavirus infection (including rabies) in animals and humans is based on laboratory confirmation. The reference techniques for post-mortem rabies diagnosis are still based on direct immunofluorescence and virus isolation, but
L. Dacheux, F. Larrous, R. Lavenir, Anthony Lepelletier, A. Faouzi, Cécile Troupin, J. Nourlil, P. Buchy, H. Bourhy   +8 more
semanticscholar   +1 more source

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane, Callewaert, Nico, De Koker, Andries   +2 more
core   +2 more sources

The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.

Plant Biotechnology Journal, 2008
Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date.
L. Gutierrez, Mélanie Mauriat, S. Guénin, J. Pelloux, J. Lefebvre, Romain Louvet, C. Rustérucci, T. Moritz, F. Guerineau, C. Bellini, O. Van Wuytswinkel   +10 more
semanticscholar   +1 more source

A quantitative reverse transcription-polymerase chain reaction for detection of Getah virus [PDF]

Scientific Reports, 2018
AbstractGetah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection is essential for timely implementation of disease prevention and control interventions. Thus, a rapid and accurate nucleic acid detection method for GETV is highly needed.
Sing‐Sin Sam, Boon‐Teong Teoh, Cheah-Mun Chee, Noor-Adila Mohamed-Romai-Noor, Juraina Abd‐Jamil, Shih Keng Loong, Chee‐Sieng Khor, Kim‐Kee Tan, Sazaly AbuBakar   +8 more
openaire   +3 more sources

Colorimetric Assessment of BCR-ABL1 Transcripts in Clinical Samples Via Gold Nanoprobes [PDF]

, 2016
Gold nanoparticles functionalized with thiolated oligonucleotides (Au-nanoprobes) have been used in a range of applications for the detection of bioanalytes of interest, from ions to proteins and DNA targets.
Baptista, P, Botelho de Sousa, A, Correia, C, Fernandes, A, Lourenço, A, Ribeiro, P, Vinhas, R   +6 more
core   +1 more source

An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples

Malaria Journal, 2015
Highly sensitive, scalable diagnostic methods are needed to guide malaria elimination interventions. While traditional microscopy and rapid diagnostic tests (RDTs) are suitable for the diagnosis of symptomatic malaria infection, more sensitive tests are ...
Matthew Adams, Sudhaunshu Joshi, Gillian Mbambo, Amy Z. Mu, Shay M. Roemmich, Biraj Shrestha, Kathy Strauss, Nicole Eddington Johnson, Khine Zaw Oo, T. M. Hlaing, Z. Han, K. Han, Si Thura, A. Richards, F. Huang, M. Nyunt, C. Plowe   +16 more
semanticscholar   +1 more source