Results 21 to 30 of about 329,236 (311)

First detection of Grapevine rupestris stem pitting-associated virus and Grapevine rupestris vein feathering virus, and new phylogenetic groups for Grapevine fleck virus and Hop stunt viroid isolates, revealed from grapevine field surveys in Spain

Phytopathologia Mediterranea, 2016
Evaluation of the prevalence of virus and viroid infections was conducted in a grapevine field collection in Valencia, Spain. Samples of autochthonous and traditional grapevine cultivars were collected during November 2011 and tested for the presence of ...
Nicola FIORE, Alan ZAMORANO, Nicolás SÁNCHEZ-DIANA, Ximena GONZÁLEZ, Vicente PALLÁS, Jesús SÁNCHEZ-NAVARRO   +5 more
doaj   +1 more source

Molecular (real-time reverse transcription polymerase chain reaction) diagnosis of SARS-CoV-2 infections: complexity and challenges

Journal of Laboratory Medicine, 2021
The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first recorded in Wuhan, China.
Sethi Shneh, Chakraborty Trinad
doaj   +1 more source

Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs

Water Science and Technology, 2021
Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on ...
Manjusha Lekshmi, Sanath H. Kumar, Kooloth Valappil Rajendran, Binaya Bhusan Nayak   +3 more
doaj   +1 more source

A Simple Reverse Transcription-Polymerase Chain Reaction for Dengue Type 2 Virus Identification

Memorias do Instituto Oswaldo Cruz, 1997
We show here a simplified reverse transcription-polymerase chain reaction (RT-PCR) for identification of dengue type 2 virus. Three dengue type 2 virus strains, isolated from Brazilian patients, and yellow fever vaccine 17DD, as a negative control, were ...
Luiz Tadeu M Figueiredo, Weber Chelli Batista, Akira Igarashi   +2 more
doaj   +1 more source

The Inherent Quantitative Capacity of the Reverse Transcription-Polymerase Chain Reaction

Analytical Biochemistry, 1999
The quantitative capacity of the reverse transcription-polymerase chain reaction (RT-PCR) is generally underestimated. In this study, PCR and RT-PCR products were amplified from serially diluted DNA and RNA templates, respectively, using a 35-cycle PCR.
Viviana C. Falco, William P. Halford, Bryan M. Gebhardt, Daniel J.J. Carr   +3 more
openaire   +3 more sources

Paradigm shift in the diagnosis of peste des petits ruminants: scoping review

Acta Veterinaria Scandinavica, 2020
Peste des petits ruminants virus causes a highly contagious disease, which poses enormous economic losses in domestic animals and threatens the conservation of wild herbivores.
Edson Kinimi, Steven Odongo, Serge Muyldermans, Richard Kock, Gerald Misinzo   +4 more
doaj   +1 more source

Detecting plasma hsa_circ_0061276 in patients with gastric cancer by reverse transcription-digital polymerase chain reaction

Frontiers in Oncology, 2022
BackgroundThe role of circular RNAs (circRNAs) in the occurrence of gastric cancer is still unclear. Therefore, the diagnostic value and mechanisms underlying hsa_circ_0061276 in the occurrence of gastric cancer were explored.MethodsReverse transcription-
Yao Ruan, Yao Ruan, Zhe Li, Yaoyao Xie, Weiliang Sun, Weiliang Sun, Junming Guo, Junming Guo, Junming Guo   +8 more
doaj   +1 more source

RT-PCR (Reverse Transcription - Polymerase Chain Reaction): Suatu Cara Pendeteksi Perubahan-Perubahan Ekspresi Gen pada Penyakit

Journal of Dentistry Indonesia, 2015
The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA expression and cloning cDNA. This method consists of two parts: cDNA synthesis from mRNA by reverse transcription (RT) and amplification of cDNA by PCR. The
Elza Ibrahim Auerkari, Hari Sunarto, Ariadna Djais   +2 more
doaj   +1 more source

Reverse transcription and polymerase chain reaction: principles and applications in dentistry [PDF]

Journal of Applied Oral Science, 2004
Various molecular biology techniques have become available in the last few years. One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in 1985. This method relies on the exponential amplification of specific DNA fragments, resulting in millions of copies that
Santos, Carlos Ferreira dos, Sakai, Vivien Thiemy, Machado, Maria Aparecida de Andrade Moreira, Schippers, Daniela Nicole, Greene, Andrew Seth   +4 more
openaire   +5 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source