Results 31 to 40 of about 677,547 (352)

The Inherent Quantitative Capacity of the Reverse Transcription-Polymerase Chain Reaction

Analytical Biochemistry, 1999
The quantitative capacity of the reverse transcription-polymerase chain reaction (RT-PCR) is generally underestimated. In this study, PCR and RT-PCR products were amplified from serially diluted DNA and RNA templates, respectively, using a 35-cycle PCR.
W P, Halford, V C, Falco, B M, Gebhardt, D J, Carr   +3 more
openaire   +2 more sources

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]

, 2013
Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra, Deforce, Dieter, Heindryckx, Björn, O'Leary, Thomas, Van Neste, Christophe, Vandesompele, Jo, Vossaert, Liesbeth   +6 more
core   +2 more sources

Subacute sclerosing panencephalitis in an infant: diagnostic role of viral genome analysis. [PDF]

, 1994
Subacute sclerosing panencephalitis (SSPE) is related to "defective" measles virus or vaccination, though an association with parainfluenza viruses has been reported.
Abdelnoor, Asher, Assaad, Behan, Bellini, Bouteille, Cattaneo, Curran, Dyken, Eggerding, Godec, McCarthy, Polna, Risk, Rorke, Wender, Yalaz   +16 more
core   +1 more source

Evaluating aflatoxin gene expression in Aspergillus section Flavi [PDF]

, 2017
The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the
B Scherm, F Degola, J Heptinstall, J Yu, M Jamali, MJ Sweeney, P Rodrigues, RPM Paterson   +7 more
core   +1 more source

Predictors of response and rational combinations for the novel MCL‐1 inhibitor MIK665 in acute myeloid leukemia

Molecular Oncology, EarlyView.
This study characterizes the responses of primary acute myeloid leukemia (AML) patient samples to the MCL‐1 inhibitor MIK665. The results revealed that monocytic differentiation is associated with MIK665 sensitivity. Conversely, elevated ABCB1 expression is a potential biomarker of resistance to the treatment, which can be overcome by the combination ...
Joseph Saad, Rhiannon Newman, Elmira Khabusheva, Sofia Aakko, Eric Durand, Mahesh Tambe, Heikki Kuusanmäki, Alun Parsons, Juho J. Miettinen, Komal Kumar Javarappa, Ezgi June Olgac, Nemo Ikonen, Mika Kontro, Kimmo Porkka, Heiko Maacke, Janghee Woo, Ensar Halilovic, Caroline A. Heckman   +17 more
wiley   +1 more source

Human Coronavirus Infections in Rural Thailand: A Comprehensive Study Using Real-Time Reverse-Transcription Polymerase Chain Reaction Assays

Journal of Infectious Diseases, 2007
Background. We sought to determine whether infections with human coronaviruses (HCoVs) 229E, OC43, HKU1, and NL63 are associated with pneumonia and to define the epidemiology of HCoV infection in rural Thailand. Methods.
R. Dare, A. Fry, M. Chittaganpitch, P. Sawanpanyalert, S. Olsen, D. Erdman   +5 more
semanticscholar   +1 more source

Rapid detection of Norwalk-like viruses (NLVs) [PDF]

, 1999
Describes the development of a reverse transcription-polymerase chain reaction and an enzyme immunoassay for the detection of an Australian Norwalk-like virus group 2b virus (Camberwell) and related viruses and their application to clinical samples ...
Wati, Satiya
core  

A Polymerase-chain-reaction Assay for the Specific Identification of Transcripts Encoded by Individual Carcinoembryonic Antigen (CEA)-gene-family Members [PDF]

, 1993
Carcinoembryonic antigen (CEA) is a tumor marker that belongs to a family of closely related molecules with variable expression patterns. We have developed sets of oligonucleotide primers for the specific amplification of transcripts from individual CEA ...
Ang, Berling, Bernards, Chi, Chomczynski, Chomczynski, Daniel, F. Kommoss, Fainstein, Hinoda, Hinoda, J. Thompson, Khan, Kim, Kuroki, Lathe, Lucas, Motoyama, N. Beauchemin, Nagel, Neumaier, Ng, Oikawa, Ponte, Pèlegrin, Rooney, S. Mössinger, S. von Kleist, Sakuragi, Sambrook, Shively, Thompson, U. Engels, V. Reichardt, W. Zimmermann   +34 more
core   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]

, 2009
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E., Olawole, O., Vincken, J.P., Visser, R.G.F.   +3 more
core   +2 more sources