Results 41 to 50 of about 657,904 (378)

Nanozyme-based biosensing for clinical diagnosis of COVID-19: A mini review [PDF]

open access: yesarXiv, 2023
Several clinical methods had been utilized for diagnosis of COVID-19 for instance, real-time reverse transcription-polymerase chain reaction (rRT-PCR), hematology examination, polymerase chain reaction (PCR), diagnostic guidelines based on clinical features, and Chest CT scans.
arxiv  

COVID-19 Screening Using Residual Attention Network an Artificial Intelligence Approach [PDF]

open access: yes2020 19th IEEE International Conference on Machine Learning and Applications (ICMLA), 2020
Coronavirus Disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 virus (SARS-CoV-2). The virus transmits rapidly; it has a basic reproductive number R of 2.2-2.7. In March 2020, the World Health Organization declared the COVID-19 outbreak a pandemic.
arxiv   +1 more source

Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation [PDF]

open access: yes, 2015
The identification of chromosomal rearrangements is of utmost importance for the diagnosis and classification of specific leukaemia subtypes and therefore has an impact on therapy choices in individual cases.
Federico, C   +4 more
core   +2 more sources

Molecular (real-time reverse transcription polymerase chain reaction) diagnosis of SARS-CoV-2 infections: complexity and challenges

open access: yesJournal of Laboratory Medicine, 2021
The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first recorded in Wuhan, China.
Sethi Shneh, Chakraborty Trinad
doaj   +1 more source

Molecular diagnosis of peste des petits ruminants virus (PPR) in goats and sheep populations [PDF]

open access: yes, 2022
Peste des petits ruminants (PPR) is an economically important viral disease of goats and sheep. The disease is confused clinically with other infections such as the mild strain of rinderpest in small ruminants.
Abdel-Rady, A
core   +1 more source

A Simple Reverse Transcription-Polymerase Chain Reaction for Dengue Type 2 Virus Identification

open access: yesMemorias do Instituto Oswaldo Cruz, 1997
We show here a simplified reverse transcription-polymerase chain reaction (RT-PCR) for identification of dengue type 2 virus. Three dengue type 2 virus strains, isolated from Brazilian patients, and yellow fever vaccine 17DD, as a negative control, were ...
Luiz Tadeu M Figueiredo   +2 more
doaj   +1 more source

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction [PDF]

open access: yes, 2012
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively.
Ballhause, M.   +16 more
core   +2 more sources

Detecting plasma hsa_circ_0061276 in patients with gastric cancer by reverse transcription-digital polymerase chain reaction

open access: yesFrontiers in Oncology, 2022
BackgroundThe role of circular RNAs (circRNAs) in the occurrence of gastric cancer is still unclear. Therefore, the diagnostic value and mechanisms underlying hsa_circ_0061276 in the occurrence of gastric cancer were explored.MethodsReverse transcription-
Yao Ruan   +8 more
doaj   +1 more source

The Inherent Quantitative Capacity of the Reverse Transcription-Polymerase Chain Reaction

open access: yesAnalytical Biochemistry, 1999
The quantitative capacity of the reverse transcription-polymerase chain reaction (RT-PCR) is generally underestimated. In this study, PCR and RT-PCR products were amplified from serially diluted DNA and RNA templates, respectively, using a 35-cycle PCR.
Viviana C. Falco   +3 more
openaire   +3 more sources

Real-time reverse transcription polymerase chain reaction [PDF]

open access: yes, 2011
RT-qPCR is one of the most widespread and reliable methods of measuring gene expression. First, the extraction of mRNA from a homogeneous cell group is done in order to perform the RT phase (reverse transcription). At this point the reverse transcriptase
Ferreira, Marcus Castro   +2 more
core   +2 more sources

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