Results 61 to 70 of about 657,904 (378)

Coswara -- A Database of Breathing, Cough, and Voice Sounds for COVID-19 Diagnosis [PDF]

, 2020
The COVID-19 pandemic presents global challenges transcending boundaries of country, race, religion, and economy. The current gold standard method for COVID-19 detection is the reverse transcription polymerase chain reaction (RT-PCR) testing. However, this method is expensive, time-consuming, and violates social distancing.
arxiv   +1 more source

Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels in Nigeria, 2015 [PDF]

, 2015
Evidence of current and past Middle East respiratory syndrome coronavirus (MERS-CoV) infection in dromedary camels slaughtered at an abattoir in Kano, Nigeria in January 2015, was sought by reverse transcription-quantitative polymerase chain reaction (RT-
Chan, MS, Chu, KW, Kuranga, SA, Oladipo, JO, Peiris, JSM, Perera, RAPM, Poon, LLM   +6 more
core   +1 more source

Application of droplet digital PCR in quantitative detection of the cell-free circulating circRNAs

Biotechnology & Biotechnological Equipment, 2018
Diagnostics based on circulating circular RNA (circRNA) is an emerging field for noninvasive molecular diagnosis, owing to the stable circular structure of circRNA.
Dan-Feng Chen, Lu-Jun Zhang, Kezhe Tan, Qing Jing   +3 more
doaj   +1 more source

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Rapid detection of Norwalk-like viruses (NLVs) [PDF]

, 1999
Describes the development of a reverse transcription-polymerase chain reaction and an enzyme immunoassay for the detection of an Australian Norwalk-like virus group 2b virus (Camberwell) and related viruses and their application to clinical samples ...
Wati, Satiya
core  

Typing of Poultry Influenza Virus (H5 and H7) by Reverse Transcription- Polymerase Chain Reaction

Italian Journal of Animal Science, 2010
The ability of the influenza Orthomixovirus to undergo to continually antigenically changes that can affect its pathogenicity and its diffusion, explains the growing seriousness of this disease and the recent epizoozies in various parts of the world ...
Cesare Bonacina, Francesca Paganelli, Paolo Cordioli, Giovanni Tosi, Paola Massi   +4 more
doaj   +1 more source

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

Cell‐free and extracellular vesicle microRNAs with clinical utility for solid tumors

Molecular Oncology, EarlyView.
Cell‐free microRNAs (cfmiRs) are small‐RNA circulating molecules detectable in almost all body biofluids. Innovative technologies have improved the application of cfmiRs to oncology, with a focus on clinical needs for different solid tumors, but with emphasis on diagnosis, prognosis, cancer recurrence, as well as treatment monitoring.
Yoshinori Hayashi, Janelle‐Cheri Millen, Romela Irene Ramos, Jennifer A. Linehan, Timothy G. Wilson, Dave S. B. Hoon, Matias A. Bustos   +6 more
wiley   +1 more source

Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma [PDF]

, 2015
Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy.
Asadi, J., Ghanbari, R., Knuutila, S., Malekzadeh, R., Mohamadkhani, A., Mosakhani, N., Mowla, S.J., Nouraee, N., Poustchi, H., Yazdani, Y.   +9 more
core   +1 more source

Comparison of rRNA-based reverse transcription PCR and rDNA-based PCR for the detection of streptococci in root canal infections

Journal of Applied Oral Science
Objective The rDNA-based method is unable to distinguish between alive and dead cells. Alternatively, bacterial viability can be assessed by molecular methods based on ribosomal RNA (rRNA).
Laís Cunha PRADO, Giulio GAVINI, Amanda da Costa SILVEIRA, Vitor Cesar NAKAMURA, Marcia Pinto Alves MAYER, Ericka Tavares PINHEIRO   +5 more
doaj   +1 more source