Results 81 to 90 of about 329,236 (311)

Competitive reverse transcription-polymerase chain reaction for quantitation of feline immunodeficiency virus

Journal of Virological Methods, 1995
A competitive reverse transcription-polymerase chain reaction (RT-PCR) was developed to quantify RNA of feline immunodeficiency virus (FIV) in cats. The assay uses in vitro synthesized RNA derived from the gag region of the FIV genome as a competitive internal control.
Horzinek, M.C., Vahlenkamp, T.W., Egberink, H.F., Eijk, M.J. van, Slotboom-Kamphorst, A.M., Verschoor, E.J., Ronde, A. AD de   +6 more
openaire   +4 more sources

Retrotransposon Expression Is Upregulated in Adulthood and Suppressed during Regeneration of the Limb in the Axolotl (Ambystoma mexicanum)

Advanced Biology, EarlyView.
The axolotl's remarkable regenerative abilities decline with age, the causes may include the numerous repetitive elements within its genome. This study uncovers how Ty3 retrotransposons and coexpression networks involving muscle and immune pathways respond to aging and regeneration, suggesting that transposons respond to physiological shifts and may ...
Samuel Ruiz‐Pérez, Nicolás Alcaraz, Karla Torres‐Arciga, José Antonio Ocampo‐Cervantes, Alejandra Cervera, Clementina Castro‐Hernández, Cynthia Gabriela Sámano‐Salazar, Ernesto Soto‐Reyes, Rodrigo González‐Barrios   +8 more
wiley   +1 more source

VALIDATION OF REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION FOR INDIRECT ASSESSMENT OF FMD VIRUS TITRE

Ветеринария сегодня, 2019
Procedure for validation of the method for indirect assessment of cultural FMD virus titre (T FMDV) in raw materials used for vaccine production with real time reverse transcription polymerase chain reaction (RT-PCRrt) based on determined amplifcation ...
D. A. Lozovoy, M. I. Doronin, V. A. Starikov, D. V. Mikhalishin, A. A. Shishkova, A. M. Timina, A. B. Borisov   +6 more
doaj   +1 more source

Novel Biologically Active Glass Fiber Functionalized Using Magnesium Phosphate Cement Promotes Bone and Vascular Regeneration

Advanced Biology, EarlyView.
In this study, a new type of bioactive glass fiber ‐based composite magnesium phosphate bone cement is prepared and verified that its mechanical strength and biological properties. In addition, the cement may have played a biologically active role in the Notch and HIF signaling pathways.
Yuzheng Lu, Yanbo Shan, Yingjie Xiong, Jianting Ye, Yanbin Wu, Jipeng Song, Yao Zhang, Wancheng Lin, Haoye Meng, Wenjing Xu, Jiang Peng, Qiang Lu, Lixiang Ding   +12 more
wiley   +1 more source

Alleviation of Aging‐Related Hallmarks in a Mouse Model of Progeria via a Nanoparticle‐Based Artificial Transcription Factor

Advanced Functional Materials, EarlyView.
Oct4‐nanoscript, a biomimetic nanoparticle‐based artificial transcription factor, precisely regulates cellular rejuvenation by activating Oct4 target genes, restoring epigenetic marks, and reducing DNA damage. In a progeria model, it effectively rescued aging‐associated pathologies and extended lifespan.
Hongwon Kim, Junyeop Kim, Euiyeon Lee, Brandon Conklin, Yannan Hou, Sumin Kim, Yerim Hwang, Ki‐Bum Lee, Jongpil Kim   +8 more
wiley   +1 more source

Rapid and visual detection of novel astroviruses causing fatal gout in goslings using one-step reverse transcription loop-mediated isothermal amplification

Poultry Science, 2020
To visually and rapidly detect a novel goose astrovirus (N-GoAstV) causing fatal gout in goslings, an isothermal detection method based on one-step reverse transcription loop-mediated isothermal amplification (one-step RT-LAMP) was established.
Jun Ji, Qinxi Chen, Chaoge Sui, Wen Hu, Zhengli Yu, Zhibin Zhang, Xinhao Mu, Xin Xu, Lunguang Yao, Yunchao Kan, Qingmei Xie   +10 more
doaj  

Nosocomial Acquisition of Dengue

Emerging Infectious Diseases, 2004
Recent transmission of dengue viruses has increased in tropical and subtropical areas and in industrialized countries because of international travel.
Dirk Wagner, Katja de With, Daniela Huzly, Frank Hufert, Manfred Weidmann, Susanne Breisinger, Sabine Eppinger, Winfried Vinzent Kern, Tilman Martin Bauer   +8 more
doaj   +1 more source

Detection of ALL-1/AF4 fusion transcript by reverse transcription- polymerase chain reaction for diagnosis and monitoring of acute leukemias with the t(4;11) translocation [PDF]

, 1993
Andrea Biondi, Alessandro Rambaldi, Vincenzo Rossi, L Elia, Corrado Caslini, Giuseppe Basso, R Battista, Tiziano Barbui, Franco Mandelli, Giuseppe Masera   +9 more
openalex   +1 more source

Double Cross‐Linked Hydrogel for Intra‐articular Injection as Modality for Macrophages Metabolic Reprogramming and Therapy of Rheumatoid Arthritis

Advanced Functional Materials, EarlyView.
A hydrogel for intra‐articular injection for the treatment of rheumatoid arthritis (RA) is formulated and comprehensively studied. After administration, the hydrogel induces a metabolic reprogramming of immunometabolism of macrophages to trigger fatty acid oxidation subsequently inducing polarization of anti‐inflammatory M2 macrophages. This results in
Yutong Song, Valentin A. Milichko, Ziqiao Ding, Wen Li, Bei Kang, Yunsheng Dou, Sona Krizkova, Zbynek Heger, Nan Li   +8 more
wiley   +1 more source

Oropouche Virus Isolation, Southeast Brazil

Emerging Infectious Diseases, 2005
An Oropouche virus strain was isolated from a novel host (Callithrix sp.) in Arinos, Minas Gerais State, southeastern Brazil. The virus was identified by complement fixation test and confirmed by reverse transcription–polymerase chain reaction ...
Márcio Roberto Teixeira Nunes, Lívia Carício Martins, Sueli Guerreiro Rodrigues, Jannifer Oliveira Chiang, Raimunda do Socorro da Silva Azevedo, Amelia P.A. Travassos da Rosa, Pedro Fernando da Costa Vasconcelos   +6 more
doaj   +1 more source