Results 181 to 190 of about 812,875 (226)

Detection of IL-1β gene polymorphism in CMV-infected renal failure patients using RFLP-PCR

Microbial Biosystems
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Genotypic mutation analysis by RFLP/PCR

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1993
In most cases somatic mutations in disease-related genes do not give rise to a functional change of the mutated cell which would allow its isolation or expansion in vitro. Therefore, selection of mutated cells on the basis of an altered phenotype has to be replaced by biochemical separation and detection of the altered sequence of the gene of interest.
C, Pourzand, P, Cerutti
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Strain discrimination of cultured and symbiotic Frankia by RFLP-PCR

Soil Biology and Biochemistry, 1994
Abstract A non-enzymic method is described for isolation of DNA from both cultured and symbiotic Frankia . Application of a combination of restriction enzyme digestion and electrophoresis techniques to a polymorphic fragment of the 16s rDNA gene, amplified by PCR, permitted discrimination between different Frankia genomic groups.
N.R. McEwan, C.T. Wheeler, J.J. Milner
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Detection of benzimidazole resistance in Haemonchus contortus using RFLP-PCR technique

Veterinary Parasitology, 2006
Benzimidazole (BZ) resistance in Haemonchus contortus is linked primarily with the mutation in the beta-tubulin isotype 1 gene that substitute phenylalanine (Phe) to tyrosine (Tyr) at 200 codon of the gene. In the present study, a new restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique has been developed for ...
J, Tiwari, S, Kumar, A P, Kolte, C P, Swarnkar, D, Singh, K M L, Pathak   +5 more
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Genotypic Mutation Assay (RFLP/PCR)

1996
Mutational spectrum analysis of DNA has gained considerable interest among molecular toxicologists as an attempt to correlate DNA alterations caused by endogenous and exogenous mutagens with epidemiological studies of cancer risk factors (Shields and Harris, 1991).
Fernando Aguilar, Peter Cerutti
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Snail Species Identification by RFLP‐PCR and Designing of Species‐Specific Oligonucleotide Primers

Journal of Food Science, 2001
ABSTRACT: To enforce labeling regulations in the authentication of snail species it might be of importance to use nonmorphological methods. Snail meat of 2 species was analyzed by restriction fragment length polymorphism (RFLP) of the genes encoding mitochondrial 12S rRNA and 16S rRNA.
A. Abdulmawjood, M. Bülte
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Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene

Veterinary Microbiology, 2008
Coagulase-negative staphylococci (CNS) have become the predominant pathogens causing bovine mastitis in many countries. CNS infections are associated with damage to milk secretory tissue of the mammary gland by increased connective tissue stroma, moderate increases of somatic cells count in milk and significant production decreases.
Olinda Cabral da Silva, Santos, Elaine Menezes, Barros, Maria Aparecida Vasconcelos Paiva, Brito, Maria do Carmo de Freire, Bastos, Kátia Regina Netto, Dos Santos, Marcia, Giambiagi-Demarval   +5 more
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Comparison of GenoType® MTBC with RFLP-PCR and multiplex PCR to identify Mycobacterium tuberculosis complex species

European Journal of Clinical Microbiology & Infectious Diseases, 2006
The aims of the work presented here were to evaluate a commercial system based on DNA hybridization technology on nitrocellulose strips (GenoType MTBC; Hain Diagnostika, Nehren, Germany) for identifying Mycobacterium tuberculosis complex isolates to the species level and to compare the results with those obtained using the RFLP-PCR and multiplex PCR ...
M P Romero, Gómez, L, Herrera-León, M S, Jiménez, J García, Rodríguez   +3 more
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Comparison between RFLP-PCR and antigenemia for pp65 antigen for diagnosis of cytomegalovirus disease after kidney transplantation

Transplantation Proceedings, 2004
Cytomegalovirus (CMV) infection is the single most frequent infectious complication in renal transplant recipients. The purpose of this study was to analyze the diagnostic efficacy of PCR-RFLP compared to antigenemia for CMV disease (CMVD) in kidney transplant recipients.
R, Schröeder, T, Michelon, I, Fagundes, A, Bortolotto, M, Petry, E, Lammerhirt, L, Santos, V, Cantarelli, E, Keitel, V, Garcia, J, Neumann   +10 more
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Scanning of Geotrichum candidum genome by RFLP-PCR of rDNA and RAPD

Electronic Journal of Polish Agricultural Universities, 2006
In the present work we focused on the comparison of genome diversity of 23 strains of Geotrichum candidum isolated from different sources. The analysis of restriction fragment length polymorphism after amplification of rDNA cluster with NS3-ITS4 primers (RFLP-PCR rDNA) and randomly amplified polymorphic DNA (RAPD) analysis with four primers [(GTG)5 ...
Michał Piegza, Wojciech Barszczewski, Danuta Witkowska, Regina Stempniewicz, Małgorzata Robak   +4 more
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