Results 51 to 60 of about 812,875 (226)

Hybridization With a Non‐Native Congener Threatens the Catalan Chub (Squalius laietanus): Optimization of a DNA‐Based Early Warning Tool

open access: yesIntegrative Zoology, EarlyView.
Hybridization with non‐native congeners threatens endemic freshwater fishes. Using a rapid and cost‐effective nuclear DNA assay, we detected widespread hybridization between the Catalan chub (Squalius laietanus) and the non‐native European chub (Squalius cephalus) across S.
Núria Pérez‐Bielsa   +4 more
wiley   +1 more source

A new and versatile method for the successful conversion of AFLP-TM markers into simple single locus markers [PDF]

open access: yes, 2003
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required.
Brugmans, B.W.   +4 more
core   +1 more source

Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI [PDF]

open access: yesBrazilian Journal of Microbiology, 2014
In this study we attempted to modify the PCR-RFLP method using restriction enzyme MwoI for the identification of medically important Aspergillus species. Our subjects included nine standard Aspergillus species and 205 Aspergillus isolates of approved hospital acquired infections and hospital indoor sources.
Diba, K.   +3 more
openaire   +4 more sources

Impact of FSHB and FSHR Genes Polymorphisms on Hormonal Profile and Sperm Retrieval Outcome in Men With Klinefelter Syndrome: A Clinical–Genetic Predictive Study

open access: yesAndrology, EarlyView.
ABSTRACT Background Genetic variability within the follicle‐stimulating hormone (FSH)‐related genes might contribute to phenotypic heterogeneity in patients with Klinefelter syndrome (KS), yet its clinical impact on sperm retrieval remains unclear. Objectives To investigate the association between FSHB c.211 G > T and FSHR polymorphisms (c.2039 A > G ...
Andrea Graziani   +6 more
wiley   +1 more source

PCR primers for PCR-RFLP assays.

open access: yes, 2014
PCR primers for PCR-RFLP assays.
Wei Li (7081)   +8 more
core   +1 more source

PCR-RFLP detection results of the three new SNPs (g.-1293C>G, g.56T>C, g.7017C>T) in bovine C3 gene.

open access: yes, 2022
PCR-RFLP detection results of the three new SNPs (g.-1293C>G, g.56T>C, g.7017C>T) in bovine C3 gene.
Yonghui Wang (287491)   +8 more
core   +1 more source

Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh [PDF]

open access: yesJournal of Parasitology Research, 2012
Mymensingh is the most endemic district for kala-azar in Bangladesh.Phlebotomus argentipesremains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length polymorphism (RFLP) of 18S rRNA gene of sand fly ...
Mohammad Shafiul Alam   +4 more
openaire   +3 more sources

Functional assessment of inherited myeloid neoplasm‐associated SAMD9L germline variants via Monoallelic CRISPR modelling

open access: yesBritish Journal of Haematology, EarlyView.
Summary While the majority of myeloid neoplasms are sporadic, the increasing application of germline genetic testing has led the World Health Organization to designate ‘Myeloid malignancies with germline predisposition’ as a distinct clinical entity, carrying major implications for clinical care and research.
Elena Vuelta   +9 more
wiley   +1 more source

DETERMINACIÓN DE LA MUTACIÓN S315T DEL GEN KATG EN AISLADOS RESISTENTES A ISONIACIDA DE MYCOBACTERIUM TUBERCULOSIS MEDIANTE PCR-RFLP

open access: yesInfectio, 2018
Objetive: To determine the S315T mutation of the katG gene in Mycobacterium tuberculosis isoniazid-resistant isolates by PCR-RFLP. Materials and Methods: Polymorphism analysis of 1054 and 630 bp products containing the S315T mutation of the katG gene ...
Jenny Sánchez-Domínguez
doaj   +1 more source

Continuous darkness is associated with improved growth performance during a high‐temperature period and sex‐specific modulation of early gonadal development in juvenile yellowtail (Seriola quinqueradiata)

open access: yesJournal of Fish Biology, EarlyView.
Abstract Selective breeding of yellowtail (Seriola quinqueradiata) is expanding in Japan, increasing the demand for practical reproductive control tools in commercial aquaculture to reduce genetic risks from escapees. Slowing early gonadal development through environmental manipulation may support future sterilization strategies, particularly when ...
Yuya Hasegawa   +3 more
wiley   +1 more source

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