The refolding of recombinant human liver methylmalonyl-CoA mutase from inclusion bodies produced in Escherichia coli : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]
Human methylmalonyl-CoA mutase (hMCM) is an adenosylcobalamin-dependent enzyme that catalyses the structural rearrangement of (R)-methylmalonyl-CoA to succinyl-CoA as pan of the catabolism of the branched chain amino acids valine, leucine and isoleucine,
Hayes, Michelle Marie
core
An Expanded Mechanism for Rhodanese Catalysis
Abstract The kinetic behavior of rhodanese (thiosulfate:cyanide sulfurtransferase, EC 2.8.1.1) was investigated at pH values from 5.0 to 10.8. The binding of thiosulfate was shown to be dependent upon a pK' of 9.9, with the protonated enzyme binding the substrate much more strongly than the deprotonated form. An enzymic nucleophile displaces sulfite to
P, Schlesinger, J, Westley
openaire +2 more sources
Biological hydropersulfides and related polysulfides – a new concept and perspective in redox biology [PDF]
The chemical biology of thiols (RSH, e.g., cysteine and cysteine‐containing proteins/peptides) has been a topic of extreme interest for many decades due to their reported roles in protein structure/folding, redox signaling, metal ligation, cellular ...
Akaike Takaaki +13 more
core +2 more sources
Supersulfide biology and translational medicine for disease control
Abstract For decades, the major focus of redox biology has been oxygen, the most abundant element on Earth. Molecular oxygen functions as the final electron acceptor in the mitochondrial respiratory chain, contributing to energy production in aerobic organisms. In addition, oxygen‐derived reactive oxygen species including hydrogen peroxide and nitrogen
Uladzimir Barayeu +5 more
wiley +1 more source
Pb2+ tolerance by Frankia sp. strain EAN1pec involves surface-binding [PDF]
Several Frankia strains have been shown to be lead-resistant. The mechanism of lead resistance was investigated for Frankia sp. strain EAN1pec. Analysis of the cultures by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDAX ...
Furnholm, Teal +3 more
core +2 more sources
Kinetic properties of Rhodanese from African locust bean seeds (Parkia biglobosa)
African locust bean (Parkia biglobosa) is a legume which can be processed into condiment. Rhodanese is a sulphurtransferase enzyme. It catalyses the degradation of cyanide to a less toxic metabolite, thiocyanate.
A. Ehigie +3 more
semanticscholar +1 more source
Bovine mitochondrial rhodanese is a phosphoprotein
The mitochondrial sulfurtransferase, rhodanese, has been analyzed for phosphate content. Significant amounts of protein-bound phosphate (30-40%) were measured in the six rhodanese preparations examined. Chromatographic experiments followed by phosphate analyses done on two of the preparations indicated that rhodanese A and rhodanese B, two enzyme forms
K, Ogata, X, Dai, M, Volini
openaire +2 more sources
The developed graphene‐assisted plasmonic nanocoral platform enables sensitive, label‐free surface‐enhanced Raman scattering analysis of salivary metabolites for head and neck cancer (HNC) detection. When combined with machine learning classification and nonnegative least squares‐based spectral deconvolution, this approach achieves 98% diagnostic ...
Hyo Jeong Seo +11 more
wiley +1 more source
Physicochemical properties of rhodanese from the kidney of cane rat (Thryonomys swinderianus)
We report the properties of partially purified rhodanese, a cyanide detoxifying enzyme isolated from the kidney of the greater cane rat commonly known as grasscutter (Thryonomys swinderianus). T. swinderianus kidney rhodanese had a specific activity of 1.
L. Ehigie +3 more
semanticscholar +1 more source
Bioleaching Microbial Community Metabolism and Composition Driven by Copper Sulphide Mineral Type
Bioleaching is a microbially mediated green technology for the recovery of metals from ore. Here, microbial consortia from a copper bioleaching column in Cyprus were cultivated on chalcopyrite (CuFeS2) and then sub‐cultured on chalcocite (Cu2S) and chalcopyrite to investigate how mineral type and surface chemistry influence microbial community ...
Katherine R. Lane +9 more
wiley +1 more source

