Results 21 to 30 of about 69,355 (271)

Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain

Nature Communications, 2023
Rhodopsin is a prototypical G protein-coupled receptor (GPCR) critical for vertebrate vision. Research on GPCR signaling states has been facilitated using llama-derived nanobodies (Nbs), some of which bind to the intracellular surface to allosterically ...
Arum Wu, David Salom, John D. Hong, Aleksander Tworak, Kohei Watanabe, Els Pardon, Jan Steyaert, Hideki Kandori, Kota Katayama, Philip D. Kiser, Krzysztof Palczewski   +10 more
doaj   +1 more source

C-terminal threonines and serines play distinct roles in the desensitization of rhodopsin, a G protein-coupled receptor

eLife, 2015
Rod photoreceptors generate measurable responses to single-photon activation of individual molecules of the G protein-coupled receptor (GPCR), rhodopsin.
Anthony W Azevedo, Thuy Doan, Hormoz Moaven, Iza Sokal, Faiza Baameur, Sergey A Vishnivetskiy, Kristoff T Homan, John JG Tesmer, Vsevolod V Gurevich, Jeannie Chen, Fred Rieke   +10 more
doaj   +1 more source

Retinal location and structure in squid rhodopsin [PDF]

, 2021
In order to understand retinal we calculated the dihedral angles around carbon axis IOC-12C, since two different carbon sequences 9C-10C-11C-12C and 10C-11C-12C-13C exist. We also calculated the distances between two specified carbon pairs. Those results
Kito, Masashi, Maddess, Ted, Nagai, Yoshinori   +2 more
core  

Rhodopsin expression level affects rod outer segment morphology and photoresponse kinetics. [PDF]

PLoS ONE, 2012
The retinal rod outer segment is a sensory cilium that is specialized for the conversion of light into an electrical signal. Within the cilium, up to several thousand membranous disks contain as many as a billion copies of rhodopsin for efficient photon ...
Clint L Makino, Xiao-Hong Wen, Norman A Michaud, Henry I Covington, Emmanuele DiBenedetto, Heidi E Hamm, Janis Lem, Giovanni Caruso   +7 more
doaj   +1 more source

Early stages of retinal development depend on Sec13 function [PDF]

, 2013
Summary ER-to-Golgi transport of proteins destined for the extracellular space or intracellular compartments depends on the COPII vesicle coat and is constitutive in all translationally active cells.
Cavodeassi, Florencia, Feng, Yi, Schmidt, Katy, Stephens, David J   +3 more
core   +3 more sources

The two-photon reversible reaction of the bistable jumping spider rhodopsin-1 [PDF]

, 2019
Bistable opsins are photopigments expressed in both invertebrates and vertebrates. These light-sensitive G-protein-coupled receptors undergo a reversible reaction upon illumination.
Deupi, Xavier, Ehrenberg, David, Heberle, Joachim, Koyanagi, Mitsumasa, Lesca, Elena, Schertler, Gebhard F. X., Terakita, Akihisa, Varma, Niranjan   +7 more
core   +1 more source

Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina

Bio-Protocol, 2019
Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its
Sowmya Bekshe Lokappa, M. Cornwall, Jeannie Chen   +2 more
doaj   +1 more source

Abrupt onset of mutations in a developmentally regulated gene during terminal differentiation of post-mitotic photoreceptor neurons in mice. [PDF]

PLoS ONE, 2014
For sensitive detection of rare gene repair events in terminally differentiated photoreceptors, we generated a knockin mouse model by replacing one mouse rhodopsin allele with a form of the human rhodopsin gene that causes a severe, early-onset form of ...
Ivette M Sandoval, Brandee A Price, Alecia K Gross, Fung Chan, Joshua D Sammons, John H Wilson, Theodore G Wensel   +6 more
doaj   +1 more source

Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice

iScience, 2023
Summary: Photoreception requires amplification by mammalian rhodopsin through G protein activation, which requires a visual cycle. To achieve this in retinal gene therapy, we incorporated human rhodopsin cytoplasmic loops into Gloeobacter rhodopsin ...
Yusaku Katada, Kazuho Yoshida, Naho Serizawa, Deokho Lee, Kenta Kobayashi, Kazuno Negishi, Hideyuki Okano, Hideki Kandori, Kazuo Tsubota, Toshihide Kurihara   +9 more
doaj   +1 more source

Negative regulation of the novel norpA(P24) suppressor, diehard4, in the endo-lysosomal trafficking underlies photoreceptor cell degeneration. [PDF]

PLoS Genetics, 2013
Rhodopsin has been used as a prototype system to investigate G protein-coupled receptor (GPCR) internalization and endocytic sorting mechanisms. Failure of rhodopsin recycling upon light activation results in various degenerative retinal diseases ...
Jongwoo Lee, Myungchul Song, Sujeong Hong   +2 more
doaj   +1 more source