Results 51 to 60 of about 69,355 (271)
Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing
Advanced Science, EarlyView.CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen +15 more
wiley +1 more source
Molecular logic behind the three-way stochastic choices that expand butterfly colour vision. [PDF]
, 2016 Butterflies rely extensively on colour vision to adapt to the natural world. Most species express a broad range of colour-sensitive Rhodopsin proteins in three types of ommatidia (unit eyes), which are distributed stochastically across the retina.
Arikawa, Kentaro +5 more
core +1 more source
Multimodal Layer‐Crossing Interrogation of Brain Circuits Enabled by Microfluidic Axialtrodes
Advanced Science, EarlyView.The study introduces a flexible microfluidic axialtrode that integrates optical, electrical, and chemical modalities within a single polymer fiber. By redistributing electrodes and fluidic channels along the fiber axis via angled cleaving, it enables simultaneous optogenetic stimulation, electrophysiological recording, and drug delivery across brain ...
Kunyang Sui +8 more
wiley +1 more source
Frontiers in Molecular Biosciences, 2022 It is well known that lipids neighboring integral membrane proteins directly influence their function. The opposite effect is true as well, as membrane proteins undergo structural changes after activation and thus perturb the lipidic environment.
Federico Baserga +11 more
doaj +1 more source
Eye, 1998 Two-dimensional crystals of rhodopsin were studied to determine the arrangement of the transmembrane alpha helices. A combination of electron cryomicroscopy, image processing and electron crystallography was used to extract amplitudes and phases from images, and a three-dimensional map to a resolution of 7.5 A was calculated.
openaire +3 more sources
Multiple Residues in the Second Extracellular Loop Are Critical for M3 Muscarinic Acetylcholine Receptor Activation [PDF]
, 2007 Recent studies suggest that the second extracellular loop (o2 loop) of bovine rhodopsin and other class I G protein-coupled receptors (GPCRs) targeted by biogenic amine ligands folds deeply into the transmembrane receptor core where the binding of cis ...
Kim, Soo-Kyung +3 more
core +1 more source
New spectral templates for rhodopsin and porphyropsin visual pigments [PDF]
Archives of Biological Sciences, 2019 A four-parameter model of spectral sensitivity curves was developed. Empirical equations were designed for A1- and A2-based visual pigments with the main α-band maximum absorptions (λmax) from 350 nm, near the ultraviolet, up to 635 nm in the far-red ...
Gačić Zoran +3 more
doaj +1 more source
Feedback from rhodopsin controls rhodopsin exclusion in Drosophila photoreceptors [PDF]
Nature, 2011 Sensory systems with high discriminatory power use neurons that express only one of several alternative sensory receptor proteins. This exclusive receptor gene expression restricts the sensitivity spectrum of neurons and is coordinated with the choice of their synaptic targets.
Vasiliauskas, Daniel +8 more
openaire +3 more sources
Incorporation of membrane proteins into large single bilayer vesicles. Application to rhodopsin. [PDF]
, 1979 A general procedure to incorporate membrane proteins in a native state into large single bilayer vesicles is described. The results obtained with rhodopsin from vertebrate and invertebrate retinas are presented.
Darszon, A +3 more
core
Fluctuations of Complex Networks: Electrical Properties of Single Protein Nanodevices
, 2004 We present for the first time a complex network approach to the study of the electrical properties of single protein devices. In particular, we consider an electronic nanobiosensor based on a G-protein coupled receptor.
Akimov, V. +4 more
core +1 more source