Results 221 to 230 of about 433,059 (284)

Evolution of mammalian pancreatic ribonucleases

Biosystems, 1975
Pancreatic ribonucleases form a group of homologous proteins found in considerable quantities in the pancreas of a number of mammalian taxa and a few reptiles (Barnard, 1969; Beintema et al.,1973). The ribonuclease activity varies greatly in different species.
Gerda Groen, G.Leijenaar-Van Den Berg, B.A.C. van Dijk, J.J. Beintema, M. Emmens, G.W. Welling, A.M. van den Berg, Wim Gaastra   +7 more
openaire   +4 more sources

Immunologic comparison of pancreatic ribonucleases

Immunochemistry, 1976
Abstract Ouchterlony double immunodiffusion and micro-complement fixation were used in cross-reactivity studies with 9 pancreatic ribonucleases differing 3–28% in amino acid sequence and rabbit antisera to cow, gnu, reindeer and whale ribonuclease.
Gerda Groen, Gjalt W. Welling, F.P. Schröder, Marchienus Emmens, Jaap J. Beintema   +4 more
openaire   +3 more sources

The molecular evolution of pancreatic ribonuclease

Journal of Molecular Evolution, 1977
The primary structures of pancreatic ribonucleases from 26 species (18 artiodactyls, horse, whale, 5 rodents and turtle) are known. Several species contain identical ribonucleases (cow/bison; sheep/goat), other species show polymorphism (arabian camel) or the presence of two structural gene loci (guinea pig pancreas contains two ribonucleases that ...
Jaap J. Beintema, Gjalt W. Welling, Johannes A. Lenstra, Walter M. Fitch, Wim Gaastra   +4 more
openaire   +4 more sources

Evaluation of Different Signal Peptides for Secretory Production of Recombinant Bovine Pancreatic Ribonuclease A in Gram Negative Bacterial System: an In silico Study

, 2017
DOI: 10.2174/1570164614666170725144424 Abstract: Background: Prokaryotic systems such as E. coli are among the most affordable and simplest hosts which are being employed to express recombinant proteins, nevertheless without appropriate signal peptide ...
A. Forouharmehr, M. Nassiri, S. Ghovvati, A. Javadmanesh   +3 more
semanticscholar   +1 more source

The interaction of DNA with pancreatic ribonuclease A

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
Abstract 1. 1. The complex formation of calf thymus DNA with pancreatic ribonuclease A (ribonucleate pyrimidinenucleotido-2′-transferase (cycling), EC 2.7.7.16) was studied through ultracentrifugation, competitive inhibition of ribonuclease activity and gel filtration on Sephadex G-200.
Kenji Sakaguchi, Eiichi Nakano, Hiroshi Sekine   +2 more
openaire   +3 more sources

Crystallization of mouse pancreatic ribonuclease

Journal of Molecular Biology, 1988
Mouse pancreatic ribonuclease has been crystallized in a form suitable for X-ray structure determination. The crystals grown from solutions of 2-methyl-2,4-pentanediol diffract to high resolution and belong to the hexagonal space group P6(1) (P6(5)) with unit cells dimensions a = b = 64.44 A, c = 53.91 A, y = 120 degrees and V = 1.94 x 10(5) A3 (1 A ...
Alan L. Perry, Rex A. Palmer
openaire   +3 more sources

Modification of the properties of bovine pancreatic ribonuclease A by covalent attachment of D-gluconyl-glycine residues.

International journal of peptide & protein research, 2009
Bovine pancreatic ribonuclease A was reacted with D-gluconyl-glycine azide in aqueous solution at pH 8.9, in absence of phosphates. Five out of 11 amino groups can be reproducibly modified and the penta D-gluconyl-glycinated ribonuclease A had greater ...
L. Biondi, F. Filira, V. Giormani, R. Rocchi   +3 more
semanticscholar   +1 more source

Pancreatic Ribonucleases

1997
Publisher Summary This chapter reviews recent data on several aspects of the catalytic mechanism of pancreatic RNases as well as some molecular properties such as carbohydrate content and folding and unfolding pathways. This chapter begins with a discussion on reaction catalyzed by pancreatic ribonucleases.
Maria Vilanova, M. Victòria Nogués, Claudi M. Cuchillo   +2 more
openaire   +2 more sources

Mechanism of glutathione regeneration of reduced pancreatic ribonuclease a.

International journal of peptide & protein research, 2009
A comparative study was made of the kinetics of glutathione regeneration of reduced pancreatic ribonuclease A, as determined by circular dishroism, sulfhydryl oxidation and the kinetics of reactivation. Four sulfhydryls were reozidizedeactivation.
Stephen W. Schaffer
semanticscholar   +1 more source

Isolation and characterization of monodeamidated derivatives of bovine pancreatic ribonuclease A.

International journal of peptide & protein research, 2009
The isolation and characterization of the initial intermediates formed during the irreversible acid denaturation of enzyme Ribonuclease A are described.
Y. Venkatesh, P. Vithayathil
semanticscholar   +1 more source