Results 61 to 70 of about 23,635 (226)

Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Peroxidase Freeze-Dried from Alkaline pH [PDF]

, 2001
The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C.
Hägerdal B., J. A. Saraiva, J. C. Oliveira, M. A. Lemos, Pizzocaro F., Van Loey A., Whitaker J. R.   +6 more
core   +2 more sources

5′tRF‐GlyGCC Promotes Breast Cancer Progression via LDHA‐Mediated Glycolysis and Macrophage Polarization

Advanced Science, EarlyView.
5’tRF‐GlyGCC promotes breast cancer malignancy by binding to LDHA. This interaction, facilitated by FGFR1 and LDHA phosphorylation, enhances glycolysis. Additionally, 5’tRF‐GlyGCC/LDHA signaling recruits and polarizes macrophages into a pro‐tumor M2 state via CCL7, thus remodeling the tumor microenvironment.
Cheng Yi, Yunqing Lu, Xing Chang, Ying Wang, Jiawang Zhou, Guoyou Xie, Lijun Tao, Zhaotong Wang, Yifan Tian, Lihong Wang, Feng Tang, Yijing Zheng, Xinchen Yue, Jinping Lei, Xiansong Wang, Lichen Ge, Zhuojia Chen, Hongsheng Wang   +17 more
wiley   +1 more source

Optimization of RNA Extraction from Rat Pancreatic Tissue

Iranian Journal of Medical Sciences, 2014
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any ...
Sanaz Dastgheib, Cambyz Irajie, Raheleh Assaei, Farhad Koohpeima, Pooneh Mokarram   +4 more
doaj  

The KDM6B/SLC10A2 Axis Suppresses MDSCs Recruitment via ERK/AP‐1 Signaling in Colorectal Cancer

Advanced Science, EarlyView.
In wild‐type intestinal epithelial cells, KDM6B demethylates SLC10A2 to promote its expression, after which SLC10A2 suppresses ERK phosphorylation, inhibiting AP‐1 (Fos/Jun) transcription and chemokine production. In KDM6B‐deficient intestinal epithelial cells, SLC10A2 methylation increases, and its expression decreases.
Zhibo Hu, Jing Xun, Bin Liu, Xiaolin Jiang, Yingdi Han, Huichao Yang, Qi Gao, Ruifang Gao, Aimin Zhang, Xueliang Wu, Dapeng Zhang, Dihua Li, Tian Li, Shimin Yang, Ximo Wang, Xiangyang Yu, Qi Zhang   +16 more
wiley   +1 more source

A Humanized Anti-CD22-Onconase Antibody-Drug Conjugate Mediates Highly Potent Destruction of Targeted Tumor Cells

Journal of Immunology Research, 2015
Antibody-drug conjugates (ADCs) have evolved as a new class of potent cancer therapeutics. We here report on the development of ADCs with specificity for the B-cell lineage specific (surface) antigen CD22 being expressed in the majority of hematological ...
Tobias Weber, Athanasios Mavratzas, Stefan Kiesgen, Stephanie Haase, Benedikt Bötticher, Evelyn Exner, Walter Mier, Ludger Grosse-Hovest, Dirk Jäger, Michaela A. E. Arndt, Jürgen Krauss   +10 more
doaj   +1 more source

Highly sensitive nuclease assay based on chemically-modified DNA or RNA [PDF]

, 2014
Nucleolytic enzymes are associated with various diseases, and several methods have been developed for their detection. DNase expression is modulated in such diseases as acute myocardial infarction, transient myocardial ischemia, oral cancer, stomach ...
Sato Shinobu, Takenaka Shigeori
core   +2 more sources

A20 Facilitates Oxaliplatin Sensitivity in Colorectal Cancer Through Monoubiquitylation of IKK‐β

Advanced Science, EarlyView.
This study demonstrates that the expression of A20, a ubiquitin‐editing enzyme, is positively correlated with oxaliplatin sensitivity in colorectal cancer (CRC). Mechanistically, A20 enhances oxaliplatin sensitivity by inhibiting NF‐κB nuclear translocation through monoubiquitination of IKK‐β.
Fan Luo, Ting Yang, Jiaxin Cao, Qun Chen, Zhenhai Lu, Feiteng Lu, Chaozhuo Lin, Zengfei Xia, Yuanzhong Yang, Peng Li, Wenjuan Ma, Min Luo, Rongxin Zhang   +12 more
wiley   +1 more source

Interaction of Human Pancreatic Ribonuclease with Human Ribonuclease Inhibitor [PDF]

Journal of Biological Chemistry, 2001
تتفاعل الريبونوكليزات الثديية بقوة مع مثبط الريبونوكليز داخل الخلايا. الخلايا حقيقية النواة المعرضة للريبونوكليزات الثديية محمية من تأثيرها السام للخلايا عن طريق تثبيط الريبونوكليزات داخل الخلايا بواسطة RI. يتشابه الريبونوكلياز البنكرياسي البشري (HPR) هيكليًا ووظيفيًا جدًا مع الريبونوكلياز أ البقري ويتفاعل مع الريبونوكلياز البشري ذو التقارب العالي.
Deepak Gaur, Srividya Swaminathan, Janendra K. Batra   +2 more
openaire   +1 more source

GlycoChat Uncovers Glycan–Lectin Circuits in the Tumor Microenvironment of Pancreatic Cancer

Advanced Science, EarlyView.
Aberrant glycosylation drives cancer progression, yet its role in the tumor microenvironment remains unclear. We developed GlycoChat to map glycan–lectin circuits at single‐cell resolution. We discovered that cancer cells induce immunosuppressive macrophage differentiation and impair phagocytosis through interactions with CLEC10A and SIGLEC3 ...
Dinh Xuan Tuan Anh, Sunanda Keisham, Arun Burramsetty, Lalhaba Oinam, Koichiro Kumano, Akihiro Kuno, Osamu Shimomura, Tatsuya Oda, Hiroaki Tateno   +8 more
wiley   +1 more source

Dependence of ethanol effects on protein charges [PDF]

, 2014
Ethanol is used as a conventional disinfectant solution. It is highly effective against enveloped viruses due to its effects on virus membranes. It also confers inactivation of non-enveloped viruses, which can be ascribed to conformational changes or ...
Arakawa Tsutomu, Shiraki Kentaro, Yoshizawa Shunsuke, 白木 賢太郎   +3 more
core   +1 more source