Results 141 to 150 of about 156,401 (384)
Lipid Nanoparticle‐Mediated CRISPR‐Cas13a Delivery for the Control of Bacterial Infection
Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.New formulations of lipid nanoparticles (LNPs) that can deliver nucleic acids to Gram‐negative bacteria are proposed to combat bacterial infection. The delivery of nucleic acids by LNPs is aided by LNP‐helpers which weaken the bacterial outer membrane. LNPs encapsulating the Cas13a/gRNA expression vector achieve an antibacterial effect in both in vivo ...
Bookun Kim +9 more
wiley +1 more source
Acta Agrobotanica, 2013 IAA, kinetin and ABA in a concentration of 10-7M did not cause major changes in the activity of ribonuclease in both cultivars. IAA and kinetin in a concentration of 10-5M lowered the activity of the tested enzyme, more in the 'Union' than in the ...
Halina Skoczek
doaj +1 more source
Bovine pancreatic ribonuclease: fifty years of the first enzymatic reaction mechanism.
Biochemistry, 2011 Fifty years ago, the group of Tony Mathias and Bob Rabin at University College London deduced the first mechanism for catalysis by an enzyme, ribonuclease [Findlay, D., Herries, D. G., Mathias, A. P., Rabin, B. R., and Ross, C. A.
C. M. Cuchillo +2 more
semanticscholar +1 more source
Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.The top‐performing lipid nanoparticle, incorporating a novel ionizable lipid derived from vitamin B5, demonstrates high mRNA transfection efficiency, low toxicity, favorable stability, targeted delivery to lymphoid tissues, and high immunogenicity, showing promise as a carrier for mRNA vaccines for infectious diseases and cancer.
Soyeon Yoo +11 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.Most in vitro cancer models lack cellular diversity, functional complexity, and clinical relevance. This work highlights the generation of an innovative dynamic tetraculture with autologous patient‐derived cells within a vascularized tumor‐on‐chip, as well as the recapitulation of endothelial anergy features induced by the tumor microenvironment ...
Christine Lansche +16 more
wiley +1 more source
CRYSTALLINE RIBONUCLEASE [PDF]
Journal of General Physiology, 1940 1. A crystalline enzyme capable of digesting yeast nucleic acid has been isolated from fresh beef pancreas. 2. The enzyme called "ribonuclease" is a soluble protein of albumin type. Its molecular weight is about 15,000. Its isoelectric point is in the region of pH 8.0. 3.
openaire +2 more sources
Philosophical Transactions of the Royal Society B: Biological Sciences, 2011 The gene coding for the RNA subunit of ribonuclease P (RNase P) is essential in all free-living organisms. The RNA subunit, itself, is an enzyme and, from its evolutionary tree, we can infer that it is a very ancient molecule. The specificity of this enzyme is that it cleaves other RNA molecules at the junction of single-stranded and the 5′ end of ...
openaire +2 more sources
BioTechniques, 1997 We have developed a simple and sensitive method for the rapid quantitation of mRNA from cell cultures and small tissue samples. The method combines the high sensitivity and specificity of the ribonuclease protection assay with simple handling and rapid ...
Jinghai Zhan +2 more
doaj +1 more source
Recent Applications of Mesoporous Silica Nanoparticles in Gene Therapy
Advanced Healthcare Materials, EarlyView.The review summarizes the synthesis of mesoporous silica nanoparticles (MSNs) with modifiable surface properties, functionalization strategies, mechanism of therapeutic payload release, and current applications in gene therapy, focusing on their capabilities in the targeted delivery of therapeutic nucleic acids, CRISPR‐Cas systems, and other genetic ...
Tamanna Binte Huq +4 more
wiley +1 more source
Acta Societatis Botanicorum Poloniae, 2015 The effect of GA3 on ribonuclease activity in the embryos and endosperm of barley seeds was investigated during dormancy and after its end. Incubation of dormancy grain with GA3 stimulated its germination, increased the dry weight of the embryos and ...
A. Rejowski
doaj +1 more source