Ribonuclease H Renaturation Gel Assay Using a Fluorescent-Labeled Substrate
BioTechniques, 1997 Ribonucleases H (RNases H) are enzymes that specifically degrade the RNA of RNA-DNA hybrids. These enzymes are involved in DNA replication, reverse transcription (RT) and antisense oligodeoxyribonucleotide-mediated arrest of translation.
Li-Ying Han, Wu-Po Ma, Robert J. Crouch
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Characterization of proteins by means of their buffer capacity, measured with an ISFET-based coulometric sensor-actuator system [PDF]
, 1993 Proteins form the specific selector in many biochemical sensors. A change in one of the properties of such a protein has to be detected by an appropriate transducer, which completes the biochemical sensor.
Bergveld, P., Luo, J., Olthuis, W.
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3,7-Dihydroxytropolones Inhibit Initiation of Hepatitis B Virus Minus-Strand DNA Synthesis
Molecules, 2020 Initiation of protein-primed (-) strand DNA synthesis in hepatitis B virus (HBV) requires interaction of the viral reverse transcriptase with epsilon (ε), a cis-acting regulatory signal located at the 5’ terminus of pre-genomic RNA (pgRNA), and several ...
Ellen Bak +6 more
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ConSole: using modularity of contact maps to locate solenoid domains in protein structures. [PDF]
, 2014 BackgroundPeriodic proteins, characterized by the presence of multiple repeats of short motifs, form an interesting and seldom-studied group. Due to often extreme divergence in sequence, detection and analysis of such motifs is performed more reliably on
Godzik, Adam, Hrabe, Thomas
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HIV-1 RT-Associated RNase H review
Archives of Pharmaceutical Sciences Ain Shams University, 2018 HIV-RT is an essential enzyme for HIV replication it comprises two associated functions: RNA- and DNA-dependent DNA polymerase (RDDP & DDDP) and ribonuclease H (RNase H).
Madonna M. A. Mitry +2 more
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Inferring stabilizing mutations from protein phylogenies : application to influenza hemagglutinin [PDF]
, 2009 One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions.
A Akasako +125 more
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Structure-guided approach identifies a novel class of HIV-1 ribonuclease H inhibitors: binding mode insights through magnesium complexation and site-directed mutagenesis studies. [PDF]
Medchemcomm, 2018 Poongavanam V +10 more
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Identification of novel components of Trypanosoma brucei editosomes [PDF]
, 2003 The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole +6 more
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Protein disulfide-isomerase interacts with a substrate protein at all stages along its folding pathway [PDF]
, 2014 In contrast to molecular chaperones that couple protein folding to ATP hydrolysis, protein disulfide-isomerase (PDI) catalyzes protein folding coupled to formation of disulfide bonds (oxidative folding).
A Jansens +54 more
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A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. [PDF]
, 2015 RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a
Chen, Qiu-Xia +4 more
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