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Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]
Physiologia Plantarum : An International Journal for Plant Biology, 2018 Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson +22 more
core +2 more sources
Scientific Reports, 2020 Recent technical advances related to the CRISPR/Cas9-based genome editing system have enabled sophisticated genome editing in microalgae. Although the demand for research on genome editing in microalgae has increased over time, methodological research ...
Seongsu Kang +4 more
doaj +2 more sources
Science Advances, 2022 CRISPR-Cas9 gene editing has emerged as a powerful therapeutic technology, but the lack of safe and efficient in vivo delivery systems, especially for tissue-specific vectors, limits its broad clinical applications.
Tao Wan +5 more
semanticscholar +1 more source
Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
Theranostics, 2021 CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years, however, the translation of this biotechnology into therapy has been hindered by efficient delivery of CRISPR/Cas9 materials into target cells. Direct delivery of CRISPR/
Song Zhang +3 more
semanticscholar +1 more source
Frontiers in Genome Editing, 2022 Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target editing effects, mitigation of random integration of non-native ...
Raviraj Banakar +15 more
doaj +1 more source
Membrane surfaces regulate assembly of ribonucleoprotein condensates
Nature Cell Biology, 2022 Biomolecular condensates organize biochemistry, yet little is known about how cells control the position and scale of these structures. In cells, condensates often appear as relatively small assemblies that do not coarsen into a single droplet despite ...
W. T. Snead +5 more
semanticscholar +1 more source
CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells
Nature Biotechnology, 2021 Prime Editors have been delivered using DNA or mRNA vectors. Here we demonstrate prime editing (PE) with purified ribonucleoprotein (RNP) complexes. We introduced somatic mutations in zebrafish embryos with frequencies as high as 30% and demonstrate ...
Karl Petri +13 more
semanticscholar +1 more source
Frontiers in Genome Editing, 2022 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and ...
Yingxiao Zhang +11 more
doaj +1 more source
Unknown Areas of Activity of Human Ribonuclease Dicer: A Putative Deoxyribonuclease Activity
Molecules, 2020 The Dicer ribonuclease plays a crucial role in the biogenesis of small regulatory RNAs (srRNAs) by processing long double-stranded RNAs and single-stranded hairpin RNA precursors into small interfering RNAs (siRNAs) and microRNAs (miRNAs), respectively ...
Marta Wojnicka +2 more
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Proteins Rpr2 and Pop3 increase the activity and thermal stability of yeast RNase P
RNA Biology, 2023 RNA-based enzyme RNase P is a ribonucleoprotein complex responsible primarily for 5’-maturation of tRNAs. S. cerevisiae RNase P comprises a catalytic RNA component and nine proteins. The assembly and maturation of S.
Anna Perederina +2 more
doaj +1 more source