Results 171 to 180 of about 121,107 (346)

Strand displacement‐driven CRISPR/Cas12a framework enables precise identification of low‐abundance point mutations

open access: yesVIEW, EarlyView.
We established an engineered CRISPR/Cas12a point mutation detection strategy (E‐CRISPR) for low‐abundance point mutations detection. By integrating the precise regulatory mechanism of strand displacement reaction (SDR) with the local high‐concentration probe microenvironment created by tetrahedral DNA frameworks (TDFs), it has achieved simultaneous ...
Jiaxi Mo   +11 more
wiley   +1 more source

Impact of Acute Endurance Exercise on Alternative Splicing in Skeletal Muscle

open access: yesFASEB BioAdvances, EarlyView.
Current model on the effects of acute endurance exercise on the AS regulatory mechanism with key trans‐factors/RBPs (e.g., hnRNP‐A1, ‐A3, and TIA1). ABSTRACT Alternative splicing (AS) is a highly conserved posttranscriptional mechanism, generating mRNA variants to diversify the proteome.
Alexander Ahn   +5 more
wiley   +1 more source

Conserved function of the long noncoding RNA Blnc1 in brown adipocyte differentiation

open access: yesMolecular Metabolism, 2017
Objective: Long noncoding RNAs (lncRNAs) are emerging as important regulators of diverse biological processes. Recent work has demonstrated that the inducible lncRNA Blnc1 stimulates thermogenic gene expression during brown and beige adipocyte ...
Lin Mi   +4 more
doaj  

Role of mitochondrial complex I genes in host plant expansion of Bactrocera tau (Tephritidae: Diptera) by CRISPR/Cas9 system

open access: yesInsect Science, EarlyView.
The role of 3 mitochondrial complex I gene in host expansion of Bactrocera tau was verified by CRISPR/Cas9 gene editing system. A stable homogenous strain (Btndufa7‐/‐) of B.tau was established after silencing Ndufa7. The reduced size of larvae and pupae of Ndufa7 knockout strain was first found.Then different levels of fitness and relative expression ...
Wei Shi   +3 more
wiley   +1 more source

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