Reprogramming human T cell function and specificity with non-viral genome targeting. [PDF]
, 2018 Decades of work have aimed to genetically reprogram T cells for therapeutic purposes1,2 using recombinant viral vectors, which do not target transgenes to specific genomic sites3,4.
Alkorta-Aranburu, Gorka +44 more
core
In Vitro Studies of the Prp9·Prp11·Prp21 Complex Indicate a Pathway for U2 Small Nuclear Ribonucleoprotein Activation [PDF]
, 1996 Pre-mRNA splicing takes place on a large ribonucleoprotein particle, the spliceosome which contains the five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6. In Saccharomyces cerevisiae the mRNA splicing factors, Prp9, Prp11, and Prp21,
Abelson, John +2 more
core +1 more source
Decoding RNA–Protein Interactions: Methodological Advances and Emerging Challenges
Advanced Genetics, EarlyView.This review provides a comprehensive comparison of RNA‐ and protein‐centric methodologies for investigating RNA–protein interactions, emphasizing their respective advantages, limitations, and suitable applications. It highlights recent technological innovations, outlines strategic method selection criteria, and addresses key challenges.
Wenkai Yi, Jian Yan
wiley +1 more source
Herpes simplex virus ICP27 protein directly interacts with the nuclear pore complex through NUP62, inhibiting host nucleocytoplasmic transport pathways [PDF]
, 2012 The herpes simplex virus ICP27 protein is important for the expression and nuclear export of viral mRNAs. Although several binding sites have been mapped along the ICP27 sequence for various RNA and protein partners including the transport receptor TAP ...
Arnold +83 more
core +3 more sources
Helvetica Chimica Acta, EarlyView.Proteins are intrinsically flexible and thus require an ensemble description. In abstract conformer space, ensemble diversity is easier to assess and the required ensemble size can be established. Abstract Pairwise distance root mean square deviation defines a hyperspace where conformers with similar shape reside close to each other.
Gunnar Jeschke
wiley +1 more source
Formation of Particulate Ribonucleoprotein with Enzymic Activities in vitro. [PDF]
, 1958 Agnar P. Nygaard +5 more
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The cold adapted and temperature sensitive influenza A/Ann Arbor/6/60 virus, the master donor virus for live attenuated influenza vaccines, has multiple defects in replication at the restrictive temperature [PDF]
, 2008 We have previously determined that the temperature sensitive (ts) and attenuated (att) phenotypes of the cold adapted influenza A/Ann Arbor/6/60 strain (MDV-A), the master donor virus for the live attenuated influenza A vaccines (FluMist®), are specified
Chan, Winnie +3 more
core +1 more source
CRISPR/Cas9 Knockout of Shell Matrix Protein 1 in the Slipper‐Snail Crepidula atrasolea
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.Methods for CRISPR/Cas9 shell matrix protein knockout in the snail, Crepidula atrasolea including optimizing RNP delivery and embryo husbandry, to the use of Sanger and MiSeq sequencing, and high‐content screening of embryos to characterize the loss of Shell Matrix Protein 1 (SMP1) during larval shell development.
Grant Batzel +13 more
wiley +1 more source
Regulation of polyribosome formation and protein synthesis in the uterus. Isolation of cytoplasmic ribonucleoprotein particles and the principal properties of the cell-free protein-synthesizing system [PDF]
, 1967 Ching-Sung Teng, Terrell H. Hamilton
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The Kaohsiung Journal of Medical Sciences, EarlyView.ABSTRACT Osteoarthritis (OA) is a common degenerative osteoarthropathy with an unclear pathogenesis. Circular RNA (circRNA) has been reported to be associated with OA progression. This study aims to explore the role and potential mechanism of hsa_circ_0006646 in OA. Interleukin‐1β (IL‐1β)‐induced human chondrocytes were used as the cell model of OA. RT‐
Ming‐Yu Hua +3 more
wiley +1 more source