Results 151 to 160 of about 26,288 (294)

Environmental DNA‐based RPA‐CRISPR/Cas12a assay for on‐site detection of chironomid larvae in aquatic environments

open access: yesPest Management Science, EarlyView.
An environmental DNA (eDNA)‐based recombinase polymerase amplification (RPA)‐CRISPR/Cas12a assay enables rapid, specific, and on‐site detection of chironomids, supporting early warning, and ecological monitoring in freshwater environments. Abstract BACKGROUND Freshwater chironomid midges pose a significant threat to drinking water quality and ...
Kyuhyeong Kim   +5 more
wiley   +1 more source

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

open access: yesSmall, EarlyView.
The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa   +4 more
wiley   +1 more source

Cas12Fold Accurately Predicts Cas12 Nuclease Structures to Enable Structure‐based Genome‐editing Engineering

open access: yesSmall Structures, EarlyView.
Cas12Fold, a deep‐learning framework tailored to Cas12 nucleases, uses evolutionary signals and iterative structure‐based alignment to resolve conformational complexity. This method outperforms general models in predicting key domains and states, accurately modeling enzymes such as Casλ.
Feng Xu   +12 more
wiley   +1 more source

Distinct 5′ UTR Requirements for Translation of the Bicistronic X/P mRNA Among Avian Orthobornaviruses

open access: yesMicrobiology and Immunology, EarlyView.
ABSTRACT Orthobornaviruses express X and the phosphoprotein (P) from a bicistronic X/P mRNA, and these proteins regulate polymerase activity. In mammalian orthobornaviruses, the 5′ untranslated region (5′ UTR) of the X/P mRNA controls the translational balance between X and P and thereby promotes efficient replication.
Meng‐Chi Wu   +7 more
wiley   +1 more source

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