Results 161 to 170 of about 24,238 (294)

FAST-CRISPR: Fusogenic Association and Secured Transfection of CRISPR/Cas9 Ribonucleoproteins Using Lipid-Silica Hybrid Nanoparticles for Therapeutic Genome Editing. [PDF]

Small
Kim M, Kim K, Lee J, Lee S, Choi S, Park SA, Jeong E, Choi SY, Park HH, Park TE, Kwon T, Myung K, Yoo J, Cho SW, Joo J.   +14 more
europepmc   +1 more source

rDNAmine: A New Tool for the Analysis of Long Repetitive Sequences

Yeast, EarlyView.
This work introduces a novel approach for analysing long repetitive genomic sequences, combining a method to isolate large DNA molecules from individual chromosomes. We also describe the bioinformatics tool rDNAmine, which enables the isolation of long‐repeat sequences from reads obtained using the Oxford Nanopore method, as well as their preliminary ...
Agnieszka Czarnocka‐Cieciura, Natalia Gumińska   +1 more
wiley   +1 more source

Transgene-free genome editing in citrus and poplar meristem tissues via biolistic ribonucleoprotein delivery of CRISPR-Cas9. [PDF]

Plant Cell Rep
Rocha DC, Omoregbee MO, Luo W, Fang H, Ye Q, Liu Y, Li G, Mascoveto J, de Souza AA, Coleman G, Culver JN, Qi Y.   +11 more
europepmc   +1 more source

Maltase 1 regulates DENV2 infection and life history in Aedes aegypti

Insect Science, EarlyView.
Our study employed CRISPR/Cas9 to generate a MAL1 knockout strain of Aedes aegypti, demonstrating that the absence of MAL1 significantly suppresses DENV2 replication in the midgut and impairs viral transmission. The mutation also altered life‐history (hatching/pupation/emerging rates and sex‐specific survival), identifying MAL1 as a novel target for ...
Man‐Jin Li, Jin Cheng, Ya‐Ming Zou, Quan‐Chao Liu, Ding Zhu, Ce‐Jie Lan   +5 more
wiley   +1 more source

The CoREST Complex Regulates Alternative Splicing by the Transcriptional Regulation of RNA Processing Genes in Melanoma Cells. [PDF]

Cells
Khan AA, Aptekmann AA, Min D, Povelaitis MC, Lee S, Ryu B.   +5 more
europepmc   +1 more source

CRISPR/Cas9‐mediated Genome‐editing Reveals 10 Testis‐enriched Genes and One Non‐testis‐enriched Gene are Dispensable for Male Fecundity in Mice

Andrology, EarlyView.
ABSTRACT Background More than 1000 genes have been identified as predominantly expressed in the human testis. Advances in gene editing technologies have enabled the rapid and efficient generation of genetically engineered mice. This approach facilitates the screening of genes essential for spermatogenesis by analyzing knockout mouse models.
Yumiao Qiu, Keisuke Shimada, Masahito Ikawa   +2 more
wiley   +1 more source

Illuminating "the dark side of the HBB": Visualizing the full spectrum of Cas9 outcomes. [PDF]

Mol Ther Nucleic Acids
Scalisi G, Laurent M, Amendola M.
europepmc   +1 more source

Testis‐Enriched F‐Box Protein FBXO39 Is Important for Spermiogenesis and Male Fertility in Mice

Andrology, EarlyView.
ABSTRACT Background The SCF (Skp–Cullin–F‐box) complex is a major class of E3 ubiquitin ligases. F‐box proteins constitute the SCF complex and play a critical role in recognizing substrates for ubiquitination. In mice, several F‐box proteins, including FBXO36 and FBXO39, are predominantly expressed in testes.
Yuki Kaneda, Haruhiko Miyata, Masahito Ikawa   +2 more
wiley   +1 more source

Making order from disorder in the nucleolus. [PDF]

Genes Dev
Baserga SJ.
europepmc   +1 more source

Compartmentalisation in cAMP signalling: A phase separation perspective

British Journal of Pharmacology, EarlyView.
Cells rely on precise spatiotemporal control of signalling pathways to ensure functional specificity. The compartmentalisation of cyclic AMP (cAMP) and protein kinase A (PKA) signalling enables distinct cellular responses within a crowded cytoplasmic space.
Milda Folkmanaite, Manuela Zaccolo
wiley   +1 more source