Results 51 to 60 of about 8,043 (217)

DNA Flap‐Mediated Control of Transcription for Programmable RNA Synthesis

Angewandte Chemie, Volume 138, Issue 18, 27 April 2026.
Pyrimidine‐rich ssDNA flaps on the T7 promoter inhibit T7 RNA polymerase (T7RP) transcription. Leveraging this pyrimidine bias, we developed DNAzyme‐ and MNAzyme‐mediated flap promoter induced transcription control (D‐FIT and M‐FIT) systems. These platforms enable regulated transcription and sequence‐specific detection, highlighting the programmable ...
Eun Sung Lee, Jisu Woo, Seokjoon Kim, Seok Hyeon Kim, Gun Haeng Lee, Ki Soo Park   +5 more
wiley   +2 more sources

Alkbh8 Regulates Selenocysteine-Protein Expression to Protect against Reactive Oxygen Species Damage [PDF]

, 2015
Environmental and metabolic sources of reactive oxygen species (ROS) can damage DNA, proteins and lipids to promote disease. Regulation of gene expression can prevent this damage and can include increased transcription, translation and post translational
Begley, Thomas J., Begley, Ulrike, Clark, Ryan, Dedon, Peter C., Dziergowska, Agnieszka, Endres, Lauren, Gu, Chen, Małkiewicz, Andrzej, Melendez, J. Andres   +8 more
core   +3 more sources

Synthesis and Antiviral Evaluation of 5-(4-Aryl-1,3-butadiyn-1-yl)-uridines and Their Phosphoramidate Pronucleotides

Molecules
The emergence of RNA viruses driven by global population growth and international trade highlights the urgent need for effective antiviral agents that can inhibit viral replication.
Evan Saillard, Otmane Bourzikat, Koffi Assa, Vincent Roy, Luigi A. Agrofoglio   +4 more
doaj   +1 more source

Identification and codon reading properties of 5-cyanomethyl uridine, a new modified nucleoside found in the anticodon wobble position of mutant haloarchaeal isoleucine tRNAs [PDF]

, 2013
Most archaea and bacteria use a modified C in the anticodon wobble position of isoleucine tRNA to base pair with A but not with G of the mRNA. This allows the tRNA to read the isoleucine codon AUA without also reading the methionine codon AUG.
Babu, I. Ramesh, Blum, Paul, Chan, Tsz Yan Clement, Dedon, Peter C., Koehrer, Caroline, Kuwahara, Masayasu, Liu, Yuchen, Mandal, Debabrata, Rajbhandary, Uttam L., Soll, Dieter, Su, Dan   +10 more
core   +1 more source

Isolation and characterisation of mesenchymal stem cells from rat bone marrow and the endosteal niche: A comparative study [PDF]

, 2018
Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs).
Alraies, Amr, Battersby, Paul, Moseley, Ryan, Sloan, Alastair J, Waddington, Rachel J., Yusop, Norhayati   +5 more
core   +4 more sources

Deoxyribo‐ and Ribonucleoside H‐Phosphonates [PDF]

Current Protocols in Nucleic Acid Chemistry, 2001
AbstractMost methods for preparing H‐phosphonate monoesters suffer from variable yields and are often incompatible with common protecting groups used in oligonucleotide synthesis. This unit describes four procedures that consistently give high yields of the desired products.
Stawinski, Jacek, Strömberg, Roger
openaire   +3 more sources

Second Generation of Antisense Oligonucleotides: From Nuclease Resistance to Biological Efficacy in Animals

CHIMIA, 1996
From efforts to improve the biophysical properties of antisense oligonucleotides by incorporating backbone- or sugar-modified nucleoside analogs, 2'-O-methoxyethyl ribonucleosides 8b were identified as building blocks for a second generation of ...
Karl-Heinz Altmann, Henri Sasmor, Uwe Pieles, Judy Phillipsd, Paul Nicklind, Fram;ois Natt, Marcel Miiller, Brett P.B Monia, Pierre Martina, Dieter A Hiisken, Robert Hanera, Thomas Geiger, Susan M. Freier, Doriano Fabbro, Nicholas M. Dean, Heinz E Moser   +15 more
doaj   +2 more sources

A functional genomics approach to establish the complement of carbohydrate transporters in Streptococcus pneumoniae. [PDF]

PLoS ONE, 2012
The aerotolerant anaerobe Streptococcus pneumoniae is part of the normal nasopharyngeal microbiota of humans and one of the most important invasive pathogens.
Alessandro Bidossi, Laura Mulas, Francesca Decorosi, Leonarda Colomba, Susanna Ricci, Susanna Ricci, Gianni Pozzi, Josef Deutscher, Carlo Viti, Marco Rinaldo Oggioni   +9 more
doaj   +1 more source

Permeability of Coxiella burnetii to ribonucleosides [PDF]

Microbiology, 2002
Knowledge about transport in Coxiella burnetii, an obligate phagolysosomal parasite, is incomplete. The authors investigated the capability of isolated, intact, host-free Coxiella to transport ribonucleosides while incubated at a pH value typical of lysosomes.
Jeffrey D, Miller, Herbert A, Thompson
openaire   +2 more sources

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source