Results 171 to 180 of about 246,508 (214)

Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is a widely used delivery vehicle in gene therapy. A scalable production technology is essential for its wide clinical applications. We have taken a synthetic biology approach to generate HEK293‐based cell lines which harbor integrated genetic elements encoding essential AAV and adenoviral helper ...
Han‐Jung Kuo, Prahalad Srinivasan, Yu‐Chieh Lin, Min Lu, Carissa Rungkittikhun, Qi Zhang, Wei‐Shou Hu   +6 more
wiley   +1 more source

Cell cycle specific, differentially tagged ribosomal proteins to measure phase specific transcriptomes from asynchronously cycling cells. [PDF]

Sci Rep
Cochran JD, Leathers TA, Maldosevic E, Siejda KW, Vitello J, Lee H, Bradley LA, Young A, Jomaa A, Wolf MJ.   +9 more
europepmc   +1 more source

N6‐methyladenosine‐regulated exosome biogenesis orchestrates an immunosuppressive pre‐metastatic niche in gastric cancer peritoneal metastasis

Cancer Communications, EarlyView.
Abstract Background Gastric cancer peritoneal metastasis is clinically challenging, given the limited treatment options and poor prognosis. The molecular mechanisms that precede gastric cancer peritoneal metastasis, known as the pre‐metastatic niche (PMN), and its relationship with N6‐methyladenosine (m6A) modification remain unclear.
Song Li, Jianyuan Zhou, Shuang Wang, Qian Yang, Shulun Nie, Chunwang Ji, Xue Zhang, Shuhan Li, Xuanyu Zhou, Jiahui Chu, Xuehui Wu, Jianqiao Jiao, Ruitao Xu, Qian Xu, Miao Huang, Qiushi Wang, Liliang Dou, Qinqin Hu, Fan Jiang, Xin Dai, Zhaodi Nan, Xinyu Song, Di Zhang, Lian Liu   +23 more
wiley   +1 more source

Piper auritum (Kunth) Fungal Endophytes: A Source of Metabolites to Inhibit the Formation of Advanced Glycation End Products

Chemistry &Biodiversity, EarlyView.
Endophytic fungi of P. auritum were evaluated for their ability to produce inhibitors of formation of advanced glycation end products (AGEs). Neopestalotiopsis sp. H2 and Diaporthe sp. H5 extracts were the most active. So, we performed their chemical profiling by untargeted and phenolics‐targeted metabolomic approaches to identify the potential ...
Lory Sthephany Rochín‐Hernández, José Antonio Guerrero‐Analco, Juan Luis Monribot‐Villanueva, Luis Bernardo Flores‐Cotera   +3 more
wiley   +1 more source

Poly(ethylene Glycol)‐Based Peptidomimetics (Pegtides) of Antimicrobial Peptides

ChemBioChem, EarlyView.
Polyethylene glycol (PEG) is commonly used to improve the pharmacokinetics and pharmacodynamics of therapeutic candidates by conjugation. Here PEG is exploited for peptidomimetic conversion of antimicrobial peptides (AMPs), as candidate antibacterial agents, by replacing their polyamide backbone. The pegtides obtained thereby can be readily synthesized
Conor Shine, John R. F. B. Connolly, Robert D. Murphy, Hazel Lafferty, Abdalmalek Alfnikh, Ned P. Buijs, Hawraa Shahrour, Nathaniel I. Martin, Eoghan O'Neill, George Amarandei, Jimmy Muldoon, Marc Maresca, Deirdre Fitzgerald‐Hughes, Marc Devocelle   +13 more
wiley   +1 more source

Exploring the Substrate Flexibility of GrsB Thioesterase Leads to the Structural Reassignment of a Gramicidin S Variant

ChemBioChem, Accepted Article.
Gramicidin S (GS) is a cyclic decapeptide derived from two pentapeptides. The C‐terminal thioesterase (TE) domain of gramicidin S synthetase B (GrsB) dimerizes precursor pentapeptides and cyclizes the resulting linear decapeptide. Recently, a GS variant (GS‐SA), in which a single D‐Phe was replaced by L‐Ser(Allyl), was reported via precursor‐directed ...
Sho Konno, Tomoe Mizuguchi, Atsuko Suzuki, Miyu Tanaka, Fumihiro Ishikawa, Akihiro Taguchi, Atsuhiko Taniguchi, Genzoh Tanabe, Yoshio Hayashi   +8 more
wiley   +1 more source

TRiC Is a Structural Component of Mammalian Sperm Axonemes

Cytoskeleton, EarlyView.
ABSTRACT The TRiC chaperonin is responsible for folding ~5%–10% of the proteome in eukaryotic cells. Our recent cryo‐electron microscopy studies of axonemes from diverse mammalian cell types led to the surprising discovery that a fully assembled TRiC chaperonin is a structural component of mammalian sperm flagella, where it is tethered to the radial ...
Alan Brown, Miguel Ricardo Leung, Tzviya Zeev‐Ben‐Mordehai, Rui Zhang   +3 more
wiley   +1 more source

Implications of m⁵C modifications in ribosomal proteins on oxidative stress, metabolic reprogramming, and immune responses in patients with mid-to-late-stage head and neck squamous cell carcinoma: Insights from nanopore sequencing. [PDF]

Heliyon
Lin G, Cai H, Hong Y, Yao M, Ye W, Li W, Liang W, Feng S, Lv Y, Ye H, Cai C, Cai G.   +11 more
europepmc   +1 more source

Protein synthesis by ribosomes

, 2015
openaire   +2 more sources