Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA [PDF]
Ribosomal RNA undergoes various modifications to optimize ribosomal structure and expand the topological potential of RNA. The most common nucleotide modifications in ribosomal RNA (rRNA) are pseudouridylations and 2'-O methylations (Nm), performed by H ...
Entian, Karl-Dieter+5 more
core +1 more source
Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction
Background Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly ...
Zachary T. Herbert+20 more
doaj +1 more source
Idiosyncratic evolution of conserved eukaryote proteins that are similar in sequence to archaeal or bacterial proteins [PDF]
Sequence comparisons have been made between the proteins of 571 prokaryote species including 46 archaea and 525 bacteria and the set of human proteins.
Roy J. Britten
core +2 more sources
The Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, and Stroke-like Episode Syndrome-associated Human Mitochondrial tRNALeu(UUR) Mutation Causes Aminoacylation Deficiency and Concomitant Reduced Association of mRNA with Ribosomes [PDF]
The pathogenetic mechanism of the mitochondrial tRNALeu(UUR) A3243G transition associated with the mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome has been investigated in transmitochondrial cell lines ...
Attardi, Giuseppe+4 more
core +1 more source
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.
Although the applicability of small subunit ribosomal RNA (16S rRNA) sequences for bacterial classification is now well accepted, the general use of these molecules has been hindered by the technical difficulty of obtaining their sequences. A protocol is
D. Lane+5 more
semanticscholar +1 more source
Ribosomes and ribosomal RNA as chaperones for folding of proteins
Provocative recent reports indicate that the large subunits of either prokaryotic or eukaryotic ribosomes have the capacity to promote refolding of denatured enzymes.Salt-washed Escherichia coli ribosomes are shown to promote refolding of denatured rhodanese.
Gisela Kramer+3 more
openaire +3 more sources
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser+11 more
wiley +1 more source
Ribosomal history reveals origins of modern protein synthesis. [PDF]
The origin and evolution of the ribosome is central to our understanding of the cellular world. Most hypotheses posit that the ribosome originated in the peptidyl transferase center of the large ribosomal subunit.
Ajith Harish, Gustavo Caetano-Anollés
doaj +1 more source
Effects of alcohol and PARP inhibition on RNA ribosomal engagement in cortical excitatory neurons
We report on the effects of ethanol (EtOH) and Poly (ADP-ribose) polymerase (PARP) inhibition on RNA ribosomal engagement, as a proxy for protein translation, in prefrontal cortical (PFC) pyramidal neurons.
Harish R. Krishnan+10 more
doaj +1 more source
Systematic identification of gene families for use as markers for phylogenetic and phylogeny- driven ecological studies of bacteria and archaea and their major subgroups [PDF]
With the astonishing rate that the genomic and metagenomic sequence data sets are accumulating, there are many reasons to constrain the data analyses.
Eisen, Jonathan A.+2 more
core +4 more sources