Molecular Mechanism of DNA Topoisomerase I-Dependent rDNA Silencing: Sir2p Recruitment at Ribosomal Genes [PDF]
, 2016 Saccharomyces cerevisiae sir2Δ or top1Δ mutants exhibit similar phenotypes involving ribosomal DNA, including (i) loss of transcriptional silencing, resulting in non-coding RNA hyperproduction from cryptic RNA polymerase II promoters; (ii) alterations ...
Bjornsti, Mary Ann +6 more
core +1 more source
Protocol dependence of sequencing-based gene expression measurements. [PDF]
PLoS ONE, 2011 RNA Seq provides unparalleled levels of information about the transcriptome including precise expression levels over a wide dynamic range. It is essential to understand how technical variation impacts the quality and interpretability of results, how ...
Tal Raz +5 more
doaj +1 more source
The human methyltransferase ZCCHC4 catalyses N6-methyladenosine modification of 28S ribosomal RNA
Nucleic Acids Research, 2019 RNA methylations are essential both for RNA structure and function, and are introduced by a number of distinct methyltransferases (MTases). In recent years, N6-methyladenosine (m6A) modification of eukaryotic mRNA has been subject to intense studies, and
R. Pinto +15 more
semanticscholar +1 more source
Structure of the Ribosomal RNA Decoding Site Containing a Selenium-Modified Responsive Fluorescent Ribonucleoside Probe. [PDF]
, 2017 Comprehensive understanding of the structure-function relationship of RNA both in real time and at atomic level will have a profound impact in advancing our understanding of RNA functions in biology.
Boerneke, Mark A +3 more
core +1 more source
2′-O-Methylation of Ribosomal RNA: Towards an Epitranscriptomic Control of Translation?
Biomolecules, 2018 Ribosomal RNA (rRNA) undergoes post-transcriptional modification of over 200 nucleotides, predominantly 2′-O-methylation (2′-O-Me). 2′-O-Methylation protects RNA from hydrolysis and modifies RNA strand flexibility but does not contribute to Watson-Crick ...
P. Monaco +3 more
semanticscholar +1 more source
THE SELECTIVE INTERRUPTION OF NUCLEOLAR RNA SYNTHESIS IN HELA CELLS BY CORDYCEPIN [PDF]
, 1969 Cordycepin is an analogue of adenosine lacking the 3'-OH. When incorporated into a growing RNA molecule, cordycepin prevents further elongation, thus producing a prematurely terminated RNA molecule.
Penman, Sheldon +2 more
core +3 more sources
Protocol for Metatranscriptomic analysis of Intestinal Microbiota [PDF]
, 2012 The objective of this publication is to provide the detailed protocol for metartancriptomisc studies of animal intestinal microbiota. The protocol describes isolation of high quality microbial community RNA from the mammalian intestinal content ...
Alvaro Hernandez +4 more
core +2 more sources
Effects of alcohol and PARP inhibition on RNA ribosomal engagement in cortical excitatory neurons
Frontiers in Molecular Neuroscience, 2023 We report on the effects of ethanol (EtOH) and Poly (ADP-ribose) polymerase (PARP) inhibition on RNA ribosomal engagement, as a proxy for protein translation, in prefrontal cortical (PFC) pyramidal neurons.
Harish R. Krishnan +10 more
doaj +1 more source
Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination [PDF]
Proc. Natl. Acad. Sci. USA 105, 18159-18164 (2008), 2008 In fast growing bacteria, ribosomal RNA (rRNA) is required to be transcribed at very high rates to sustain the high cellular demand on ribosome synthesis. This results in dense traffic of RNA polymerases (RNAP). We developed a stochastic model, integrating results of single-molecule and quantitative in vivo studies of E.
arxiv +1 more source
Reconstitution of Functional Eukaryotic Ribosomes fromDictyostelium discoideum Ribosomal Proteins and RNA [PDF]
, 1997 40 and 60 S ribosomal subunits have been reconstituted in vitro from purified ribosomal RNA and ribosomal proteins of Dictyostelium discoideum. The functionality of the reconstituted ribosomes was demonstrated in in vitro mRNA-directed protein synthesis.
Giorgio Mangiarotti, Sara Chiaberge
core +1 more source