Results 91 to 100 of about 411,205 (272)

Molecular and Cellular Hallmarks of Age‐Related Vestibular Hair Cell Degeneration

Advanced Science, EarlyView.
This study utilizes single‐cell RNA‐seq transcriptomes, advanced imaging, and electrophysiology to examine universal and cell‐type‐specific aging signatures of vestibular hair cells. The study shows that impaired hair bundle function is a key driver of age‐related vestibular dysfunction.
Samadhi Kulasooriya, Huizhan Liu, Sarath Vijayakumar, Celia Bloom, Zhenhang Xu, Shu Tu, Benjamin J. Borgmeier, Mi Zhou, Litao Tao, Bechara Kachar, David Z. He   +10 more
wiley   +1 more source

Following Ribosome Footprints to Understand Translation at a Genome Wide Level

Computational and Structural Biotechnology Journal, 2018
Protein translation is a key step in gene expression. The development of Ribosome Profiling has allowed the global analysis of this process at sub-codon resolution.
Guillermo Eastman, Pablo Smircich, José R. Sotelo-Silveira   +2 more
doaj   +1 more source

Simple and inexpensive ribosome profiling analysis of mRNA translation.

, 2018
The development and application of ribosome profiling has markedly advanced our understanding of ribosomes and mRNA translation. The experimental approach, which relies on deep sequencing of ribosome-protected mRNA fragments generated by treatment of ...
Nicchitta, Christopher V, Shenolikar, Shirish, Reid, David W   +2 more
core   +1 more source

An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome [PDF]

, 2017
Recent advances in global translatome analysis technologies enable us to understand how translational regulation of gene expression modulates cellular functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to
Hyun Yong, Jin, Changchun, Xiao
openaire   +2 more sources

Biomimetic Nanoplatform for Dual Target Nano‐Metabolic Therapy in Diabetes‐Associated Biofilm Infections

Advanced Science, EarlyView.
A biomimetic GOx/Arg‐loaded nanoplatform produces nitric oxide through an in situ catalytic cascade reaction, enabling dual‐target metabolic regulation in diabetes‐associated biofilm infections. Bacterial metabolism is disrupted, and macrophage immunometabolism is reprogrammed, resulting in biofilm eradication, immune restoration, and enhanced ...
Mingzhang Li, Yonglong Li, Jiren Yan, Changming Wang, Jinlong Yu, Feng Jiang, Boyong Wang, Yi Yang, Deyi Ding, Jin Tang, Pei Han, Botao Song, Geyong Guo, Hao Shen   +13 more
wiley   +1 more source

RPLP2 Mediates the Beneficial Effects of Exercise on Stress Resistance Through Muscle–Brain Communication

Advanced Science, EarlyView.
A novel exercise‐inducible myokine acidic ribosomal protein P2 (RPLP2), initially identified from human trials, is presented here, whose circulating levels negatively correlate with clinical anxiety severity. Muscle‐derived RPLP2 enhances hippocampal ribosomal assembly and adult neurogenesis to rescue stress‐induced anxiety deficits.
Peiyu Luo, Wei Wu, Huan Peng, Dan He, Yuxi Guo, Boyue Zhao, Xiaodan Wang, Li Ma, Yuhang Qin, Yifang Zhai, Lixia Zhuo, Ying Zhang, Yijie Guo, Linlin Jing, Fangyao Chen, Erfei Zhang, Wei Wang, Xiancang Ma, Yan Li   +18 more
wiley   +1 more source

riboviz: software for analysis and visualization of ribosome profiling datasets

, 2020
Ribosome profiling provides a detailed global snapshot of protein synthesis in a cell. At its core, this technique makes use of the observation that a translating ribosome protects around 30 nucleotides of the mRNA from nuclease activity. High-throughput
Kostas Kavoussanakis (9567940), Joshua Plotkin (9097588), Anna Kurowska (11202511), Junchen Liu (380902), Edward Wallace (9097573), Amanda Mok (9097585), Peter Tilton (11202515), Siyin Xue (11202501), Premal Shah (9097584), Sophie Winterbourne (11202509), Alexander L. Cope (8872655), Felicity Anderson (647017), Vikram Shivakumar (11202524), Liana Lareau (9097582), John Favate (9097593), Emma MacKenzie (11202506), Bala Desinghu (9097610), Tongji Xing (475878), Michael Jackson (4733808), Oana Carja (9097621)   +19 more
core   +1 more source

SiDT1 Defines Plant Architecture Reminiscent of Green Revolution in Foxtail Millet

Advanced Science, EarlyView.
SiDT1 encodes a GA3‐oxidase that creates a semi‐dwarf, lodging‐resistant architecture reminiscent of the rice Green Revolution. The resulting ideotype performs well under dense planting and provides a valuable genetic resource for high‐yield, mechanized foxtail millet production. ABSTRACT Foxtail millet (Setaria italica) is a drought‐tolerant C4 cereal
Jianzhen Lv, Jinjin Cheng, Zhen Hu, Qian Lan, Yanjun Yang, Chengcheng Pei, Junhao Luo, Zhuang Li, Peiyong Xin, Jijun Yan, Jinfang Chu, Qian Qian, Zhaosheng Kong, Liang Jiang   +13 more
wiley   +1 more source

Genome-wide assessment of differential translations with ribosome profiling data

Nature Communications, 2016
The global measurement of ribosome occupancy on mRNAs is commonly used as a proxy in estimating rates of protein synthesis. Here the authors describe Xtail, a computational approach that facilitates the extraction of accurate quantitative insight from ...
Zhengtao Xiao, Qin Zou, Yu Liu, Xuerui Yang   +3 more
doaj   +1 more source

Functionalized Biomimetic Scaffolds for Human‐Derived Auditory Neural Circuit Construction

Advanced Science, EarlyView.
In Vitro Auditory Circuit Model. ABSTRACT Damage to auditory circuits results in sensorineural hearing loss. However, the scarcity of human inner ear tissue significantly hinders the development of therapies to preserve auditory function, creating a critical need for reliable in vitro models.
Pan Feng, Qian Zhu, Yusong Wang, Hao Rong, Xu Zhang, Fuchun Wang, Tianqi Yu, Wenxuan Wang, Jing Li, Lei Tian, Menghui Liao, Renjie Chai, Yangnan Hu   +12 more
wiley   +1 more source