Results 71 to 80 of about 411,205 (272)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Ribosome profiling in mammalian cells to reveal the role of NMD factors in translation termination

, 2016
Eukaryotes have acquired numerous ways to control the integrity and quality of mRNA. Nonsense-mediated mRNA decay (NMD) is one of these conserved pathways that contribute to mRNA surveillance.
Annibaldis, Giuditta
core   +1 more source

Integrative detection of genome-wide translation using iRibo

STAR Protocols
Summary: Ribosome profiling is a sequencing technique that provides a global picture of translation across a genome. Here, we present iRibo, a software program for integrating any number of ribosome profiling samples to obtain sensitive inference of ...
Alistair Turcan, Jiwon Lee, Aaron Wacholder, Anne-Ruxandra Carvunis   +3 more
doaj   +1 more source

Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, and Applications to Understand the Mechanism of Human Diseases

Cells, 2022
RNA-seq has been widely used as a high-throughput method to characterize transcript dynamic changes in a broad context, such as development and diseases.
Atefeh Bagheri, Artem Astafev, Tara Al-Hashimy, Peng Jiang   +3 more
doaj   +1 more source

Ribonuclease selection for ribosome profiling

, 2017
Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms.
Gladyshev, Vadim, Gerashchenko, Maxim
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

HeLa ribosome profiling data

<p>This repository hosts a comprehensive dataset comprising 132 HeLa cell ribosome profiling samples from 20 distinct studies, serving as a resource for assessing the consistency and reliability of ribosome profiling measurements.</p> <p ...
Liu, ORCID
core   +2 more sources

High-Resolution Analysis of Coronavirus Gene Expression by RNA Sequencing and Ribosome Profiling.

PLoS Pathogens, 2016
Members of the family Coronaviridae have the largest genomes of all RNA viruses, typically in the region of 30 kilobases. Several coronaviruses, such as Severe acute respiratory syndrome-related coronavirus (SARS-CoV) and Middle East respiratory syndrome-
Nerea Irigoyen, Andrew E Firth, Joshua D Jones, Betty Y-W Chung, Stuart G Siddell, Ian Brierley   +5 more
doaj   +1 more source

Single‐Cell Dissection of Therapy‐Induced Remodeling Uncovers a Fibroblast‐Driven Immunosuppressive Niche and Targetable Vulnerabilities in Lethal Prostate Cancer

Advanced Science, EarlyView.
Single‐cell longitudinal profiling reveals that androgen‐deprivation therapy induces a DPT+ fibroblast‐complement axis that suppresses macrophage inflammation and drives CD8+ T cell exhaustion in prostate cancer. Concurrently, resistant epithelial subpopulations persist and engage TSPAN1‐ and NRXN1‐mediated programs promoting CRPC and neuroendocrine ...
Yang Chen, Dandan Dong, Jinling Liao, Naikai Liao, Mengqi Liu, Qin Zhang, Siting Chen, Qi Liu, Ying Lu, Tianyu Li, Chunlin Zou, Qiong Song, Qiuyan Wang, Jian Liang, Qiji Xie, Chengyang Li, Mengyun Wang, Jiwen Cheng, Zengnan Mo, Gong‐Hong Wei   +19 more
wiley   +1 more source

Selective ribosome profiling to study interactions of translating ribosomes in yeast

Nature Protocols, 2019
A number of enzymes, targeting factors and chaperones engage ribosomes to support fundamental steps of nascent protein maturation, including enzymatic processing, membrane targeting and co-translational folding. The selective ribosome profiling (SeRP) method is a new tool for studying the co-translational activity of maturation factors that provides ...
Carla V. Galmozzi, Dorina Merker, Ulrike A. Friedrich, Kristina Döring, Günter Kramer   +4 more
openaire   +3 more sources