Results 71 to 80 of about 47,955 (276)
Advanced Functional Materials, EarlyView.A swelling‐programmed micropatterned hydrogel guides adherent cells through a controlled transition from cell–matrix anchoring to cadherin‐mediated cell–cell compaction, enabling rapid assembly of high‐viability spheroids with defined size and morphology.
Han Gyeol Nam +8 more
wiley +1 more source
Integrative detection of genome-wide translation using iRibo
STAR ProtocolsSummary: Ribosome profiling is a sequencing technique that provides a global picture of translation across a genome. Here, we present iRibo, a software program for integrating any number of ribosome profiling samples to obtain sensitive inference of ...
Alistair Turcan +3 more
doaj +1 more source
Cells, 2022 RNA-seq has been widely used as a high-throughput method to characterize transcript dynamic changes in a broad context, such as development and diseases.
Atefeh Bagheri +3 more
doaj +1 more source
Illuminating Parasite Protein Production by Ribosome Profiling [PDF]
Trends in Parasitology, 2016 While technologies for global enumeration of transcript abundance are well-developed, those that assess protein abundance require tailoring to penetrate to low-abundance proteins. Ribosome profiling circumvents this challenge by measuring global protein production via sequencing small mRNA fragments protected by the assembled ribosome.
Marilyn, Parsons, Peter J, Myler
openaire +2 more sources
<p>This repository hosts a comprehensive dataset comprising 132 HeLa cell ribosome profiling samples from 20 distinct studies, serving as a resource for assessing the consistency and reliability of ribosome profiling measurements.</p> <p ...
Liu, ORCID
core +2 more sources
Advanced Science, EarlyView.Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu +12 more
wiley +1 more source
High-Resolution Analysis of Coronavirus Gene Expression by RNA Sequencing and Ribosome Profiling.
PLoS Pathogens, 2016 Members of the family Coronaviridae have the largest genomes of all RNA viruses, typically in the region of 30 kilobases. Several coronaviruses, such as Severe acute respiratory syndrome-related coronavirus (SARS-CoV) and Middle East respiratory syndrome-
Nerea Irigoyen +5 more
doaj +1 more source
Biomolecules, 2021 Ribosome profiling reveals the translational dynamics of mRNAs by capturing a ribosomal footprint snapshot. Growing evidence shows that several long non-coding RNAs (lncRNAs) contain small open reading frames (smORFs) that are translated into functional ...
Bernardo Bonilauri +2 more
doaj +1 more source
Advanced Science, EarlyView.ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch +4 more
wiley +1 more source
Selective ribosome profiling to study interactions of translating ribosomes in yeast
Nature Protocols, 2019 A number of enzymes, targeting factors and chaperones engage ribosomes to support fundamental steps of nascent protein maturation, including enzymatic processing, membrane targeting and co-translational folding. The selective ribosome profiling (SeRP) method is a new tool for studying the co-translational activity of maturation factors that provides ...
Carla V. Galmozzi +4 more
openaire +3 more sources