Results 61 to 70 of about 411,205 (272)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Ribosome profiling reveals translational regulation of mammalian cells in response to hypoxic stress

BMC Genomics, 2017
Background Retinal pigment epithelium (RPE) cells transfer oxygen and nutrients from choroid to the neural retina. Reduced oxygen to RPE perturbs development and functions of blood vessels in retina.
Zhiwen Jiang, Jiaqi Yang, Aimei Dai, Yuming Wang, Wei Li, Zhi Xie   +5 more
doaj   +1 more source

Ribosome profiling uncovers selective mRNA translation associated with eIF2 phosphorylation in erythroid progenitors. [PDF]

PLoS ONE, 2018
The regulation of translation initiation factor 2 (eIF2) is important for erythroid survival and differentiation. Lack of iron, a critical component of heme and hemoglobin, activates Heme Regulated Inhibitor (HRI). This results in phosphorylation of eIF2
Nahuel A Paolini, Kat S Moore, Franca M di Summa, Ivo F A C Fokkema, Peter A C 't Hoen, Marieke von Lindern   +5 more
doaj   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

A systematically-revised ribosome profiling method for bacteria reveals pauses at single-codon resolution

eLife, 2019
In eukaryotes, ribosome profiling provides insight into the mechanism of protein synthesis at the codon level. In bacteria, however, the method has been more problematic and no consensus has emerged for how to best prepare profiling samples.
Fuad Mohammad, Rachel Green, Allen R Buskirk   +2 more
doaj   +1 more source

mTORC1 Enhances Early Phase Ribosome Processivity

Frontiers in Molecular Biosciences, 2020
During translation elongation, the ribosome serially adds amino acids to a growing polypeptide over many rounds of catalysis. The ribosome remains bound to mRNAs over these multiple catalytic cycles, requiring high processivity. Despite its importance to
Erin An, Kyle Friend
doaj   +1 more source

Identification of the prebiotic translation apparatus within the contemporary ribosome [PDF]

, 2009
A structural element that could have existed independently in the prebiotic era was identified at the active site of the contemporary ribosome. It is suggested to have functioned as a proto-ribosome catalyzing peptide bond formation and non-coded ...
Chen Davidovich, Ada Yonath, Ilana Agmon, Anat Bashan   +3 more
core  

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

40S ribosome biogenesis co-factors are essential for gametophyte and embryo development [PDF]

, 2013
Ribosome biogenesis is well described in Saccharomyces cerevisiae. In contrast only very little information is available on this pathway in plants. This study presents the characterization of five putative protein co-factors of ribosome biogenesis in ...
Roman Martin, Martin, Roman, Roman Martin (85483), Simm, Stefan, Markus T. Bohnsack, Markus T Bohnsack, Benjamin L. Weis (277837), Stefan Simm, Sandra Missbach (277836), Markus T. Bohnsack (277840), Benjamin L Weis, Enrico Schleiff, Weis, Benjamin L., Sandra Missbach, Bohnsack, Markus, Mißbach, Sandra, Stefan Simm (277838), Schleiff, Enrico, Enrico Schleiff (49377), Lafontaine, Denis, Bohnsack, Markus T., Ra Missbach, Benjamin L. Weis   +22 more
core   +2 more sources

Ribosome A and P sites revealed by length analysis of ribosome profiling data [PDF]

Nucleic Acids Research, 2015
The high-throughput sequencing of nuclease-protected mRNA fragments bound to ribosomes, a technique known as ribosome profiling, quantifies the relative frequencies with which different regions of transcripts are translated. This technique has revealed novel translation initiation sites with unprecedented scope and has furthered investigations into the
Martens, Andrew T., Taylor, James, Hilser, Vincent J.   +2 more
openaire   +2 more sources