Results 61 to 70 of about 47,955 (276)
Ribosome profiling reveals translational regulation of mammalian cells in response to hypoxic stress
BMC Genomics, 2017 Background Retinal pigment epithelium (RPE) cells transfer oxygen and nutrients from choroid to the neural retina. Reduced oxygen to RPE perturbs development and functions of blood vessels in retina.
Zhiwen Jiang +5 more
doaj +1 more source
riboviz: software for analysis and visualization of ribosome profiling datasets
, 2020 Ribosome profiling provides a detailed global snapshot of protein synthesis in a cell. At its core, this technique makes use of the observation that a translating ribosome protects around 30 nucleotides of the mRNA from nuclease activity. High-throughput
Kostas Kavoussanakis (9567940) +19 more
core +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
eLife, 2019 In eukaryotes, ribosome profiling provides insight into the mechanism of protein synthesis at the codon level. In bacteria, however, the method has been more problematic and no consensus has emerged for how to best prepare profiling samples.
Fuad Mohammad +2 more
doaj +1 more source
mTORC1 Enhances Early Phase Ribosome Processivity
Frontiers in Molecular Biosciences, 2020 During translation elongation, the ribosome serially adds amino acids to a growing polypeptide over many rounds of catalysis. The ribosome remains bound to mRNAs over these multiple catalytic cycles, requiring high processivity. Despite its importance to
Erin An, Kyle Friend
doaj +1 more source
Ribosome profiling uncovers selective mRNA translation associated with eIF2 phosphorylation in erythroid progenitors. [PDF]
PLoS ONE, 2018 The regulation of translation initiation factor 2 (eIF2) is important for erythroid survival and differentiation. Lack of iron, a critical component of heme and hemoglobin, activates Heme Regulated Inhibitor (HRI). This results in phosphorylation of eIF2
Nahuel A Paolini +5 more
doaj +1 more source
Ribonuclease selection for ribosome profiling
, 2017 Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms.
Gladyshev, Vadim, Gerashchenko, Maxim
core +1 more source
Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance
Aging and Cancer, EarlyView.NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang +3 more
wiley +1 more source
mQC : a post-mapping data exploration tool for ribosome profiling [PDF]
, 2018 Background and objective: Ribosome profiling is a recent next generation sequencing technique enabling the genome-wide study of gene expression in biomedical research at the translation level.
Steven Verbruggen +3 more
core +1 more source
Inferring efficiency of translation initiation and elongation from ribosome profiling [PDF]
, 2020 One of the main goals of ribosome profiling is to quantify the rate of protein synthesis at the level of translation. Here, we develop a method for inferring translation elongation kinetics from ribosome profiling data using recent advances in the ...
Szavits-Nossan, Juraj +2 more
core +1 more source