Results 61 to 70 of about 78,844 (296)

Stochastic theory of protein synthesis and polysome: ribosome profile on a single mRNA transcript

, 2011
The process of polymerizing a protein by a ribosome, using a messenger RNA (mRNA) as the corresponding template, is called {\it translation}. Ribosome may be regarded as a molecular motor for which the mRNA template serves also as the track.
Agirrezabala, Ajeet K. Sharma, Alberts, Antal, Arava, Arava, Basu, Chou, Chou, Chowdhury, Chowdhury, Ciandrini, Debashish Chowdhury, Derrida, Frank, Garai, Gilchrist, Guo, Hager, Hager, Harrington, Hirokawa, Hopfield, Ingolia, Kolomeisky, Krug, Lakatos, Lodish, MacDonald, MacDonald, Mikamo, Mukamel, Ninio, Noll, Popkov, Reynolds, Rich, Romero, Schadschneider, Schütz, Sharma, Sharma, Shaw, Shaw, Shaw, Spirin, Spirin, Thompson, Warner, Warner, Zhdanov, Zia, Zouridis   +52 more
core   +1 more source

Signal recognition particle binds to translating ribosomes before emergence of a signal anchor sequence. [PDF]

, 2017
The bacterial signal recognition particle (SRP) is part of the machinery that targets ribosomes synthesizing membrane proteins to membrane-embedded translocons co-translationally. Recognition of nascent membrane proteins occurs by virtue of a hydrophobic
Holtkamp, W., Mercier, E., Rodnina, M., Wintermeyer, W.   +3 more
core   +2 more sources

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Decoding Viral Infection by Ribosome Profiling [PDF]

Journal of Virology, 2015
ABSTRACT Ribosome profiling is an emerging technique that uses deep sequencing to monitor translation in live cells. Studies using ribosome profiling have already provided novel insights into the identities and amounts of the proteins being produced in cells, as well as novel insights into the mechanism of protein synthesis and translation ...
openaire   +2 more sources

Predicting the Flexibility Profile of Ribosomal RNAs [PDF]

Molecular Informatics, 2010
AbstractFlexibility in biomolecules is an important determinant of biological functionality, which can be measured quantitatively by atomic Debye–Waller factor or B‐factor. Although numerous works have been addressed on theoretical and computational studies of the B‐factor profiles of proteins, the methods used for predicting B‐factor values of nucleic
Feifei, Tian, Chun, Zhang, Xia, Fan, Xue, Yang, Xi, Wang, Huaping, Liang   +5 more
openaire   +2 more sources

Ribosome recycling is not critical for translational coupling in Escherichia coli

eLife, 2020
We used ribosome profiling to characterize the biological role of ribosome recycling factor (RRF) in Escherichia coli. As expected, RRF depletion leads to enrichment of post-termination 70S complexes in 3′-UTRs.
Kazuki Saito, Rachel Green, Allen R Buskirk   +2 more
doaj   +1 more source

Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons [PDF]

, 2017
We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain
Dougherty, Joseph D
core   +2 more sources

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Quantitative profiling of initiating ribosomes in vivo [PDF]

Nature Methods, 2014
Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control.
Xiangwei Gao, Ji Wan, Botao Liu, Ming Ma, Ben Shen, Shu-Bing Qian   +5 more
openaire   +2 more sources

RiboA: a web application to identify ribosome A-site locations in ribosome profiling data

BMC Bioinformatics, 2021
Background Translation is a fundamental process in gene expression. Ribosome profiling is a method that enables the study of transcriptome-wide translation.
Danying Shao, Nabeel Ahmed, Nishant Soni, Edward P. O’Brien   +3 more
doaj   +1 more source