Adaptive laboratory evolution of a genome-reduced Escherichia coli. [PDF]
, 2019 Synthetic biology aims to design and construct bacterial genomes harboring the minimum number of genes required for self-replicable life. However, the genome-reduced bacteria often show impaired growth under laboratory conditions that cannot be ...
Cho, Byung-Kwan +8 more
core +2 more sources
Multiplexing polysome profiling experiments to study translation in Escherichia coli.
PLoS ONE, 2019 Polysome profiling is a widely used method to monitor the translation status of mRNAs. Although it is theoretically a simple technique, it is labor intensive.
Huong Le Nguyen +4 more
doaj +1 more source
Quantitative nucleotide level analysis of regulation of translation in response to depolarization of cultured neural cells [PDF]
, 2017 Studies on regulation of gene expression have contributed substantially to understanding mechanisms for the long-term activity-dependent alterations in neural connectivity that are thought to mediate learning and memory.
Dalal, Jasbir S +5 more
core +2 more sources
A Rapid Ribosome Profiling Method Elucidates Chloroplast Ribosome Behavior in Vivo [PDF]
The Plant Cell, 2013 Abstract The profiling of ribosome footprints by deep sequencing has revolutionized the analysis of translation by mapping ribosomes with high resolution on a genome-wide scale. We present a variation on this approach that offers a rapid and cost-effective alternative for the genome-wide profiling of chloroplast ribosomes.
Zoschke, R., Watkins, K., Barkan, A.
openaire +3 more sources
Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. [PDF]
, 2016 Rocaglamide A (RocA) typifies a class of protein synthesis inhibitors that selectively kill aneuploid tumour cells and repress translation of specific messenger RNAs.
Floor, Stephen N +2 more
core +1 more source
Translational regulation contributes to the elevated CO2 response in two Solanum species. [PDF]
, 2020 Understanding the impact of elevated CO2 (eCO2 ) in global agriculture is important given climate change projections. Breeding climate-resilient crops depends on genetic variation within naturally varying populations.
Brady, Siobhan M +6 more
core +2 more sources
Discovery of noncanonical translation initiation sites through mass spectrometric analysis of protein N termini [PDF]
, 2017 Translation initiation generally occurs at AUG codons in eukaryotes, although it has been shown that non-AUG or non-canonical translation initiation can also occur.
Barbhuiya, Mustafa +10 more
core +1 more source
Molecular Oncology, EarlyView.Tumors contain diverse cellular states whose behavior is shaped by context‐dependent gene coordination. By comparing gene–gene relationships across biological contexts, we identify adaptive transcriptional modules that reorganize into distinct vulnerability axes.
Brian Nelson +9 more
wiley +1 more source
Illuminating Parasite Protein Production by Ribosome Profiling [PDF]
Trends in Parasitology, 2016 While technologies for global enumeration of transcript abundance are well-developed, those that assess protein abundance require tailoring to penetrate to low-abundance proteins. Ribosome profiling circumvents this challenge by measuring global protein production via sequencing small mRNA fragments protected by the assembled ribosome.
Marilyn, Parsons, Peter J, Myler
openaire +2 more sources
Clarifying the Translational Pausing Landscape in Bacteria by Ribosome Profiling
Cell Reports, 2016 The rate of protein synthesis varies according to the mRNA sequence in ways that affect gene expression. Global analysis of translational pausing is now possible with ribosome profiling.
Fuad Mohammad +3 more
doaj +1 more source