Results 41 to 50 of about 411,205 (272)
eLife, 2020 Ribosomal profiling has shed new light on how ribosomes can ignore stop codons in messenger RNA.
Kim M Keeling, David M Bedwell
doaj +1 more source
Normalized Ribo-Seq for Quantifying Absolute Global and Specific Changes in Translation
Bio-Protocol, 2022 Ribosome profiling (Ribo-Seq) is a highly sensitive method to quantify ribosome occupancies along individual mRNAs on a genome-wide scale. Hereby, ribosome-protected fragments (= footprints) are generated by nuclease digestion, isolated, and sequenced ...
Katharina Hoerth +2 more
doaj +1 more source
Protein folding on the ribosome studied using NMR spectroscopy [PDF]
, 2013 NMR spectroscopy is a powerful tool for the investigation of protein folding and misfolding, providing a characterization of molecular structure, dynamics and exchange processes, across a very wide range of timescales and with near atomic resolution.
Christodoulou, J +9 more
core +1 more source
Ribosome Profiling: Global Views of Translation [PDF]
Cold Spring Harbor Perspectives in Biology, 2018 The translation of messenger RNA (mRNA) into protein and the folding of the resulting protein into an active form are prerequisites for virtually every cellular process and represent the single largest investment of energy by cells. Ribosome profiling-based approaches have revolutionized our ability to monitor every step of protein synthesis in vivo ...
Nicholas T, Ingolia +2 more
openaire +2 more sources
Characterizing inactive ribosomes in translational profiling [PDF]
Translation, 2016 The broad impact of translational regulation has emerged explosively in the last few years in part due to the technological advance in genome-wide interrogation of gene expression. During mRNA translation, the majority of actively translating ribosomes exist as polysomes in cells with multiple ribosomes loaded on a single transcript.
Botao, Liu, Shu-Bing, Qian
openaire +2 more sources
eLife, 2022 Protein methylation occurs predominantly on lysine and arginine residues, but histidine also serves as a methylation substrate. However, a limited number of enzymes responsible for this modification have been reported.
Eriko Matsuura-Suzuki +12 more
doaj +1 more source
Quantitative profiling of initiating ribosomes in vivo [PDF]
Nature Methods, 2014 Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control.
Xiangwei Gao +5 more
openaire +2 more sources
Predicting the Flexibility Profile of Ribosomal RNAs [PDF]
Molecular Informatics, 2010 AbstractFlexibility in biomolecules is an important determinant of biological functionality, which can be measured quantitatively by atomic Debye–Waller factor or B‐factor. Although numerous works have been addressed on theoretical and computational studies of the B‐factor profiles of proteins, the methods used for predicting B‐factor values of nucleic
Feifei, Tian +5 more
openaire +2 more sources
Multiplexing polysome profiling experiments to study translation in Escherichia coli.
PLoS ONE, 2019 Polysome profiling is a widely used method to monitor the translation status of mRNAs. Although it is theoretically a simple technique, it is labor intensive.
Huong Le Nguyen +4 more
doaj +1 more source
Computational methods for ribosome profiling data analysis [PDF]
, 2019 Since the introduction of the ribosome profiling technique in 2009 its popularity has greatly increased. It is widely used for the comprehensive assessment of gene expression and for studying the mechanisms of regulation at the translational level.
Kiniry, Stephen J. +5 more
core +1 more source