Results 21 to 30 of about 48,775 (245)
PLoS Computational Biology, 2020 Ribosome profiling, an application of nucleic acid sequencing for monitoring ribosome activity, has revolutionized our understanding of protein translation dynamics.
Jordan A Berg +7 more
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The awesome power of ribosome profiling [PDF]
RNA, 2015 Ribosome profiling has proved to be a fantastic innovation which has revealed so much about mRNA translation that had previously gone unrecognized. The RNA community owes Jonathan Weissman and Nick Ingolia a deep sense of gratitude for conceiving the approach in the first place, and for their detailed optimization of the methodology. The origins of the
Jackson, Richard, Standart, Nancy
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Ribosome Profiling of
mSystems, 2018 Cyanobacteria experience both rapid and periodic fluctuations in light and inorganic carbon (Ci) and have evolved regulatory mechanisms to respond to these, including extensive posttranscriptional gene regulation. We report the first genome-wide ribosome
Jan Karlsen +4 more
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Development of a tissue-specific ribosome profiling approach in Drosophila enables genome-wide evaluation of translational adaptations. [PDF]
PLoS Genetics, 2017 Recent advances in next-generation sequencing approaches have revolutionized our understanding of transcriptional expression in diverse systems. However, measurements of transcription do not necessarily reflect gene translation, the process of ultimate ...
Xun Chen, Dion Dickman
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eLife, 2014 During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome.
Liana F Lareau +3 more
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Frontiers in Molecular Biosciences, 2022 Our understanding of mRNA translation and its regulation has been transformed by the development of ribosome profiling. This approach relies upon RNase footprinting of translating ribosomes in a precise manner to generate an accurate snapshot of ribosome
Katerina Douka +7 more
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Ribosome profiling enhances understanding of mycobacterial translation
Frontiers in Microbiology, 2022 A recent addition to the -omics toolkit, ribosome profiling, enables researchers to gain insight into the process and regulation of translation by mapping fragments of mRNA protected from nuclease digestion by ribosome binding. In this review, we discuss
Elizabeth B. Sawyer, Teresa Cortes
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RiboProP: a probabilistic ribosome positioning algorithm for ribosome profiling [PDF]
Bioinformatics, 2018 Abstract Motivation Ribosome profiling has been widely used to study translation in a genome-wide fashion. It requires deep sequencing of ribosome protected mRNA fragments followed by mapping of fragments to the reference genome. For applications such as identification of ribosome pausing sites, it is
Dengke Zhao +3 more
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Genome-wide Translation Profiling by Ribosome-Bound tRNA Capture
Cell Reports, 2018 Summary: In the ribosome complex, tRNA is a critical element of mRNA translation. A rich repertoire of cell regulation is hypothesized to occur during the recruitment of specific tRNAs in polypeptide formation.
Chien-Wen Chen, Motomasa Tanaka
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High-Precision Analysis of Translational Pausing by Ribosome Profiling in Bacteria Lacking EFP
Cell Reports, 2015 Ribosome profiling is a powerful method for globally assessing the activity of ribosomes in a cell. Despite its application in many organisms, ribosome profiling studies in bacteria have struggled to obtain the resolution necessary to precisely define ...
Christopher J. Woolstenhulme +3 more
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