Results 21 to 30 of about 78,844 (296)
PLoS Computational Biology, 2020 Ribosome profiling, an application of nucleic acid sequencing for monitoring ribosome activity, has revolutionized our understanding of protein translation dynamics.
Jordan A Berg +7 more
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Ribonuclease selection for ribosome profiling [PDF]
Nucleic Acids Research, 2016 Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms. A critical issue in ribosome profiling is nuclease treatment of ribosome-mRNA complexes, as it is important to ensure both stability of ribosomal particles and
Gerashchenko, Maxim, Gladyshev, Vadim
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Ribosome Profiling of
mSystems, 2018 Cyanobacteria experience both rapid and periodic fluctuations in light and inorganic carbon (Ci) and have evolved regulatory mechanisms to respond to these, including extensive posttranscriptional gene regulation. We report the first genome-wide ribosome
Jan Karlsen +4 more
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Development of a tissue-specific ribosome profiling approach in Drosophila enables genome-wide evaluation of translational adaptations. [PDF]
PLoS Genetics, 2017 Recent advances in next-generation sequencing approaches have revolutionized our understanding of transcriptional expression in diverse systems. However, measurements of transcription do not necessarily reflect gene translation, the process of ultimate ...
Xun Chen, Dion Dickman
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The kinetic mechanism of bacterial ribosome recycling. [PDF]
, 2017 Bacterial ribosome recycling requires breakdown of the post-termination complex (PoTC), comprising a messenger RNA (mRNA) and an uncharged transfer RNA (tRNA) cognate to the terminal mRNA codon bound to the 70S ribosome.
Chen, Yuanwei +3 more
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eLife, 2014 During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome.
Liana F Lareau +3 more
doaj +1 more source
Frontiers in Molecular Biosciences, 2022 Our understanding of mRNA translation and its regulation has been transformed by the development of ribosome profiling. This approach relies upon RNase footprinting of translating ribosomes in a precise manner to generate an accurate snapshot of ribosome
Katerina Douka +7 more
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Combining in silico prediction and ribosome profiling in a genome-wide search for novel putatively coding sORFs [PDF]
, 2013 Background: It was long assumed that proteins are at least 100 amino acids (AAs) long. Moreover, the detection of short translation products (e. g.
Baggerman, Geert +6 more
core +2 more sources
RiboProP: a probabilistic ribosome positioning algorithm for ribosome profiling [PDF]
Bioinformatics, 2018 Abstract Motivation Ribosome profiling has been widely used to study translation in a genome-wide fashion. It requires deep sequencing of ribosome protected mRNA fragments followed by mapping of fragments to the reference genome. For applications such as identification of ribosome pausing sites, it is
Dengke Zhao +3 more
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The expanding toolkit of translating ribosome affinity purification [PDF]
, 2017 Translating ribosome affinity purification is a method initially developed for profiling mRNA from genetically defined cell types in complex tissues. It has been applied both to identify target molecules in cell types that are important for controlling a
Dougherty, Joseph D
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