Results 11 to 20 of about 411,205 (272)
Active Ribosome Profiling with RiboLace [PDF]
Cell Reports, 2018 Summary: Ribosome profiling, or Ribo-seq, is based on large-scale sequencing of RNA fragments protected from nuclease digestion by ribosomes. Thanks to its unique ability to provide positional information about ribosomes flowing along transcripts, this ...
Massimiliano Clamer +15 more
doaj +12 more sources
Ribosome profiling: a powerful tool in oncological research [PDF]
Biomarker ResearchNeoplastic cells need to adapt their gene expression pattern to survive in an ever-changing or unfavorable tumor microenvironment. Protein synthesis (or mRNA translation), an essential part of gene expression, is dysregulated in cancer.
Dan Su +9 more
doaj +5 more sources
Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts [PDF]
Nature CommunicationsRibosome profiling, which is based on deep sequencing of ribosome footprints, has served as a powerful tool for elucidating the regulatory mechanism of protein synthesis.
Kotaro Tomuro +9 more
doaj +4 more sources
Genome-wide Translation Profiling by Ribosome-Bound tRNA Capture
Cell Reports, 2018 Summary: In the ribosome complex, tRNA is a critical element of mRNA translation. A rich repertoire of cell regulation is hypothesized to occur during the recruitment of specific tRNAs in polypeptide formation.
Chien-Wen Chen, Motomasa Tanaka
doaj +4 more sources
riboWaltz: Optimization of ribosome P-site positioning in ribosome profiling data. [PDF]
PLoS Computational Biology, 2018 Ribosome profiling is a powerful technique used to study translation at the genome-wide level, generating unique information concerning ribosome positions along RNAs.
Fabio Lauria +5 more
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Identifying ribosome heterogeneity using ribosome profiling [PDF]
Nucleic Acids Research, 2022 Recent studies have revealed multiple mechanisms that can lead to heterogeneity in ribosomal composition. This heterogeneity can lead to preferential translation of specific panels of mRNAs, and is defined in large part by the ribosomal protein (RP ...
Alkan, Ferhat +6 more
core +8 more sources
Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments [PDF]
Nature Communications, 2021 Ribosome profiling has become the gold standard to analyze mRNA translation dynamics, and the translation inhibitor cycloheximide (CHX) is often used in its application.
Puneet Sharma +3 more
doaj +3 more sources
Protocol for Ribosome Profiling in Bacteria [PDF]
Bio-Protocol, 2019 Ribosome profiling provides information on the position of ribosomes on mRNA on a genomic scale. Although this information is often used to detect changes in gene expression under different conditions, it also has great potential for yielding insight ...
Fuad Mohammad, Allen Buskirk
doaj +3 more sources
Analysis of Ribosome Profiling Data
, 2022 Ribosome profiling methods are based on high-throughput sequencing of ribosome-protected mRNA footprints and allow to study in detail translational changes. Bioinformatic and statistical tools are necessary to analyze sequencing data.
Khanh Dao Duc +2 more
core +3 more sources
Common cell lysis procedures distort ribosome profiling analyses of gene expression [PDF]
Genome BiologyRibosome profiling is a powerful technique used to study gene expression on a transcriptome-wide scale. It involves sequencing of mRNA fragments protected by ribosomes from ribonuclease digestion. The initial steps commonly involve cell lysis followed by
Aoife O’Connell +6 more
doaj +2 more sources