Results 31 to 40 of about 48,775 (245)
Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
STAR Protocols, 2020 Summary: Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes ...
Mari Mito +2 more
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The Plant Translatome Surveyed by Ribosome Profiling [PDF]
Plant and Cell Physiology, 2019 Abstract Although transcriptome changes have long been recognized as a mechanism to induce tentative substitution of expressed genes in diverse biological processes in plants, the regulation of translation—the final step of the central dogma of molecular biology—emerged as an alternative and prominent layer in defining the output of ...
Tomoya Fujita +2 more
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eLife, 2020 Ribosomal profiling has shed new light on how ribosomes can ignore stop codons in messenger RNA.
Kim M Keeling, David M Bedwell
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In Vitro Analysis of Stalled Ribosomes using Puromycin Incorporation
Bio-Protocol, 2023 Ribosome footprint profiling has demonstrated that ribosomes can be slowed or stalled on select mRNAs, often due to the presence of rare codons, stalling motifs, or via a ribosome-binding protein (e.g., FMRP).
MaKenzie Scarpitti, Michael Kearse
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Ribosome Profiling: Global Views of Translation [PDF]
Cold Spring Harbor Perspectives in Biology, 2018 The translation of messenger RNA (mRNA) into protein and the folding of the resulting protein into an active form are prerequisites for virtually every cellular process and represent the single largest investment of energy by cells. Ribosome profiling-based approaches have revolutionized our ability to monitor every step of protein synthesis in vivo ...
Nicholas T, Ingolia +2 more
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Normalized Ribo-Seq for Quantifying Absolute Global and Specific Changes in Translation
Bio-Protocol, 2022 Ribosome profiling (Ribo-Seq) is a highly sensitive method to quantify ribosome occupancies along individual mRNAs on a genome-wide scale. Hereby, ribosome-protected fragments (= footprints) are generated by nuclease digestion, isolated, and sequenced ...
Katharina Hoerth +2 more
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Characterizing inactive ribosomes in translational profiling [PDF]
Translation, 2016 The broad impact of translational regulation has emerged explosively in the last few years in part due to the technological advance in genome-wide interrogation of gene expression. During mRNA translation, the majority of actively translating ribosomes exist as polysomes in cells with multiple ribosomes loaded on a single transcript.
Botao, Liu, Shu-Bing, Qian
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eLife, 2022 Protein methylation occurs predominantly on lysine and arginine residues, but histidine also serves as a methylation substrate. However, a limited number of enzymes responsible for this modification have been reported.
Eriko Matsuura-Suzuki +12 more
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Quantitative profiling of initiating ribosomes in vivo [PDF]
Nature Methods, 2014 Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control.
Xiangwei Gao +5 more
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Predicting the Flexibility Profile of Ribosomal RNAs [PDF]
Molecular Informatics, 2010 AbstractFlexibility in biomolecules is an important determinant of biological functionality, which can be measured quantitatively by atomic Debye–Waller factor or B‐factor. Although numerous works have been addressed on theoretical and computational studies of the B‐factor profiles of proteins, the methods used for predicting B‐factor values of nucleic
Feifei, Tian +5 more
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