Results 31 to 40 of about 47,955 (276)
eLife, 2014 During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome.
Liana F Lareau +3 more
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Frontiers in Molecular Biosciences, 2022 Our understanding of mRNA translation and its regulation has been transformed by the development of ribosome profiling. This approach relies upon RNase footprinting of translating ribosomes in a precise manner to generate an accurate snapshot of ribosome
Katerina Douka +7 more
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FredHutch/DUX4-IFNg-ribosome-footprints_Zenodo: Zenodo Release
, 2023 Ribosome footprint profiling and translation efficiency analysis for the manuscript titled "DUX4 orchestrates translational reprogramming by broadly suppressing translation ...
Chao-Jen Wong
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RiboProP: a probabilistic ribosome positioning algorithm for ribosome profiling [PDF]
Bioinformatics, 2018 Abstract Motivation Ribosome profiling has been widely used to study translation in a genome-wide fashion. It requires deep sequencing of ribosome protected mRNA fragments followed by mapping of fragments to the reference genome. For applications such as identification of ribosome pausing sites, it is
Dengke Zhao +3 more
openaire +2 more sources
Insights into translational regulation from ribosome profiling data [PDF]
, 2019 Ribosomes carry out protein synthesis from mRNA templates by a highly regulated process called translation. Within the four phases of translation - initiation, elongation, termination and recycling - the focus of translation regulation studies has ...
Chyżyńska, Katarzyna Anna
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A Comprehensive, Quantitative, and Genome-Wide Model of Translation [PDF]
, 2010 Translation is still poorly characterised at the level of individual proteins and its role in regulation of gene expression has been constantly underestimated.
Piotr Zielenkiewicz +3 more
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In Vitro Analysis of Stalled Ribosomes using Puromycin Incorporation
Bio-Protocol, 2023 Ribosome footprint profiling has demonstrated that ribosomes can be slowed or stalled on select mRNAs, often due to the presence of rare codons, stalling motifs, or via a ribosome-binding protein (e.g., FMRP).
MaKenzie Scarpitti, Michael Kearse
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High-Precision Analysis of Translational Pausing by Ribosome Profiling in Bacteria Lacking EFP
Cell Reports, 2015 Ribosome profiling is a powerful method for globally assessing the activity of ribosomes in a cell. Despite its application in many organisms, ribosome profiling studies in bacteria have struggled to obtain the resolution necessary to precisely define ...
Christopher J. Woolstenhulme +3 more
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Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
STAR Protocols, 2020 Summary: Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes ...
Mari Mito +2 more
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