Results 31 to 40 of about 411,205 (272)
F1000Research, 2020 Ribosome profiling provides the opportunity to evaluate translation kinetics at codon level resolution. Here, we describe ribosome profiling data, generated from two HEK293T cell lines.
Aikaterini Alexaki +11 more
doaj +1 more source
Ribonuclease selection for ribosome profiling [PDF]
Nucleic Acids Research, 2016 Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms. A critical issue in ribosome profiling is nuclease treatment of ribosome-mRNA complexes, as it is important to ensure both stability of ribosomal particles and
Gerashchenko, Maxim, Gladyshev, Vadim
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The awesome power of ribosome profiling [PDF]
RNA, 2015 Ribosome profiling has proved to be a fantastic innovation which has revealed so much about mRNA translation that had previously gone unrecognized. The RNA community owes Jonathan Weissman and Nick Ingolia a deep sense of gratitude for conceiving the approach in the first place, and for their detailed optimization of the methodology. The origins of the
Jackson, Richard, Standart, Nancy
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2A-induced ribosome stalling [PDF]
, 2014 Originally 2A was characterised in foot-and-mouth disease virus. Site directed mutagenesis identified a C-terminus consensus motif [D(V/I)ExNPGP] and it is proposed that 2A interacts with the exit tunnel of the ribosome in a way that a specific peptide ...
Odon, Valèrie
core +1 more source
FredHutch/DUX4-IFNg-ribosome-footprints_Zenodo: Zenodo Release
, 2023 Ribosome footprint profiling and translation efficiency analysis for the manuscript titled "DUX4 orchestrates translational reprogramming by broadly suppressing translation ...
Chao-Jen Wong
core +1 more source
A Comprehensive, Quantitative, and Genome-Wide Model of Translation [PDF]
, 2010 Translation is still poorly characterised at the level of individual proteins and its role in regulation of gene expression has been constantly underestimated.
Piotr Zielenkiewicz +3 more
core +1 more source
RiboProP: a probabilistic ribosome positioning algorithm for ribosome profiling [PDF]
Bioinformatics, 2018 Abstract Motivation Ribosome profiling has been widely used to study translation in a genome-wide fashion. It requires deep sequencing of ribosome protected mRNA fragments followed by mapping of fragments to the reference genome. For applications such as identification of ribosome pausing sites, it is
Dengke Zhao +3 more
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Insights into translational regulation from ribosome profiling data [PDF]
, 2019 Ribosomes carry out protein synthesis from mRNA templates by a highly regulated process called translation. Within the four phases of translation - initiation, elongation, termination and recycling - the focus of translation regulation studies has ...
Chyżyńska, Katarzyna Anna
core +1 more source
The Plant Translatome Surveyed by Ribosome Profiling [PDF]
Plant and Cell Physiology, 2019 Abstract Although transcriptome changes have long been recognized as a mechanism to induce tentative substitution of expressed genes in diverse biological processes in plants, the regulation of translation—the final step of the central dogma of molecular biology—emerged as an alternative and prominent layer in defining the output of ...
Tomoya Fujita +2 more
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