Results 1 to 10 of about 411,205 (272)
Ribosome profiling enhances understanding of mycobacterial translation [PDF]
Frontiers in Microbiology, 2022 A recent addition to the -omics toolkit, ribosome profiling, enables researchers to gain insight into the process and regulation of translation by mapping fragments of mRNA protected from nuclease digestion by ribosome binding. In this review, we discuss
Elizabeth B. Sawyer, Teresa Cortes
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Ribosome Profiling of
mSystems, 2018 Cyanobacteria experience both rapid and periodic fluctuations in light and inorganic carbon (Ci) and have evolved regulatory mechanisms to respond to these, including extensive posttranscriptional gene regulation. We report the first genome-wide ribosome
Jan Karlsen +4 more
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Quantitative Modeling of Protein Synthesis Using Ribosome Profiling Data
Frontiers in Molecular Biosciences, 2021 Quantitative prediction on protein synthesis requires accurate translation initiation and codon translation rates. Ribosome profiling data, which provide steady-state distribution of relative ribosome occupancies along a transcript, can be used to ...
Vandana Yadav +3 more
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PLoS Computational Biology, 2020 Ribosome profiling, an application of nucleic acid sequencing for monitoring ribosome activity, has revolutionized our understanding of protein translation dynamics.
Jordan A Berg +7 more
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Development of a tissue-specific ribosome profiling approach in Drosophila enables genome-wide evaluation of translational adaptations. [PDF]
PLoS Genetics, 2017 Recent advances in next-generation sequencing approaches have revolutionized our understanding of transcriptional expression in diverse systems. However, measurements of transcription do not necessarily reflect gene translation, the process of ultimate ...
Xun Chen, Dion Dickman
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eLife, 2014 During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome.
Liana F Lareau +3 more
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Frontiers in Molecular Biosciences, 2022 Our understanding of mRNA translation and its regulation has been transformed by the development of ribosome profiling. This approach relies upon RNase footprinting of translating ribosomes in a precise manner to generate an accurate snapshot of ribosome
Katerina Douka +7 more
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High-Precision Analysis of Translational Pausing by Ribosome Profiling in Bacteria Lacking EFP
Cell Reports, 2015 Ribosome profiling is a powerful method for globally assessing the activity of ribosomes in a cell. Despite its application in many organisms, ribosome profiling studies in bacteria have struggled to obtain the resolution necessary to precisely define ...
Christopher J. Woolstenhulme +3 more
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Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
STAR Protocols, 2020 Summary: Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes ...
Mari Mito +2 more
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In Vitro Analysis of Stalled Ribosomes using Puromycin Incorporation
Bio-Protocol, 2023 Ribosome footprint profiling has demonstrated that ribosomes can be slowed or stalled on select mRNAs, often due to the presence of rare codons, stalling motifs, or via a ribosome-binding protein (e.g., FMRP).
MaKenzie Scarpitti, Michael Kearse
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