Results 1 to 10 of about 45,683 (244)
Active Ribosome Profiling with RiboLace [PDF]
Cell Reports, 2018 Summary: Ribosome profiling, or Ribo-seq, is based on large-scale sequencing of RNA fragments protected from nuclease digestion by ribosomes. Thanks to its unique ability to provide positional information about ribosomes flowing along transcripts, this ...
Massimiliano Clamer +2 more
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Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts [PDF]
Nature CommunicationsRibosome profiling, which is based on deep sequencing of ribosome footprints, has served as a powerful tool for elucidating the regulatory mechanism of protein synthesis.
Kotaro Tomuro +9 more
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Ribosome profiling: a powerful tool in oncological research [PDF]
Biomarker ResearchNeoplastic cells need to adapt their gene expression pattern to survive in an ever-changing or unfavorable tumor microenvironment. Protein synthesis (or mRNA translation), an essential part of gene expression, is dysregulated in cancer.
Dan Su +9 more
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Ribosome Profiling of Plants. [PDF]
Methods Mol Biol, 2023 Translation is a key step in control of gene expression, yet most analyses of global responses to a stimulus focus on transcription and the transcriptome. For RNA viruses in particular, which have no DNA-templated transcriptional control, control of viral and host translation is crucial.
Sonia J, Kanodia P, Lozier Z, Miller WA.
europepmc +3 more sources
Protocol for Ribosome Profiling in Bacteria [PDF]
Bio-Protocol, 2019 Ribosome profiling provides information on the position of ribosomes on mRNA on a genomic scale. Although this information is often used to detect changes in gene expression under different conditions, it also has great potential for yielding insight ...
Fuad Mohammad, Allen Buskirk
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Advances in ribosome profiling technologies. [PDF]
Biochem Soc TransRibosome profiling (or Ribo-seq) has emerged as a powerful approach for revealing the regulatory mechanisms of protein synthesis, on the basis of deep sequencing of ribosome footprints. Recent innovations in Ribo-seq technologies have significantly enhanced their sensitivity, specificity, and resolution.
Tomuro K, Iwasaki S.
europepmc +3 more sources
Quantitative Modeling of Protein Synthesis Using Ribosome Profiling Data
Frontiers in Molecular Biosciences, 2021 Quantitative prediction on protein synthesis requires accurate translation initiation and codon translation rates. Ribosome profiling data, which provide steady-state distribution of relative ribosome occupancies along a transcript, can be used to ...
Vandana Yadav +2 more
exaly +3 more sources
Common cell lysis procedures distort ribosome profiling analyses of gene expression [PDF]
Genome BiologyRibosome profiling is a powerful technique used to study gene expression on a transcriptome-wide scale. It involves sequencing of mRNA fragments protected by ribosomes from ribonuclease digestion. The initial steps commonly involve cell lysis followed by
Aoife O’Connell +6 more
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riboWaltz: Optimization of ribosome P-site positioning in ribosome profiling data.
PLoS Computational Biology, 2018 Ribosome profiling is a powerful technique used to study translation at the genome-wide level, generating unique information concerning ribosome positions along RNAs.
Fabio Lauria +5 more
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Genome-wide assessment of differential translations with ribosome profiling data
Nature Communications, 2016 The global measurement of ribosome occupancy on mRNAs is commonly used as a proxy in estimating rates of protein synthesis. Here the authors describe Xtail, a computational approach that facilitates the extraction of accurate quantitative insight from ...
Zhengtao Xiao, Xuerui Yang, Zou Qin
exaly +2 more sources