Results 81 to 90 of about 411,205 (272)
Illuminating Parasite Protein Production by Ribosome Profiling [PDF]
Trends in Parasitology, 2016 While technologies for global enumeration of transcript abundance are well-developed, those that assess protein abundance require tailoring to penetrate to low-abundance proteins. Ribosome profiling circumvents this challenge by measuring global protein production via sequencing small mRNA fragments protected by the assembled ribosome.
Marilyn, Parsons, Peter J, Myler
openaire +2 more sources
Advanced Science, EarlyView.The pre‐regenerative vascular niche (PVN) is essential for nerve repair, yet its endothelial blueprint remains unclear. We identified angiogenic ECs (AECs) as the dominant pre‐regenerative subset and found that antler blood–derived exosomes (AB‐EXO) promote repair via IMP3.
Jinsheng Huang +11 more
wiley +1 more source
Structural studies of ribosome stalling and translocation complexes [PDF]
, 2010 In this study, cryo-electron microscopy (cryo-EM) and single particle reconstruction were used as a main technique to investigate the involvement of bacterial ribosomes in two crucial cellular processes: the regulation of gene expression and the ...
Seidelt, Birgit
core +1 more source
Ribosome Profiling, Kouloulia et al.
, 2019 .bam and .bai files of aligned ribosome profiling ...
Gkogkas, Christos
core +1 more source
The small-subunit processome is a ribosome assembly intermediate [PDF]
, 2004 The small-subunit (SSU) processome is a large ribonucleoprotein required for the biogenesis of the 18S rRNA and likely corresponds to the terminal knobs visualized by electron microscopy on the 5' end of nascent rRNAs.
Granneman, Sander; id_orcid +4 more
core +1 more source
A Streamlined Ribosome Profiling Protocol for the Characterization of Microorganisms
BioTechniques, 2015 Ribosome profiling is a powerful tool for characterizing in vivo protein translation at the genome scale, with multiple applications ranging from detailed molecular mechanisms to systems-level predictive modeling. Though highly effective, this intricate technique has yet to become widely used in the microbial research community.
Haythem Latif +6 more
openaire +3 more sources
Advanced Science, EarlyView.Integrative multi‐omics analysis delineates a mitochondrial–immune axis governing neoadjuvant chemotherapy response in high‐grade serous ovarian cancer. Immune‐active tumors exhibit enhanced B‐cell infiltration and favorable sensitivity, whereas metabolically rewired tumors display oxidative phosphorylation dependency and resistance.
Wei Jiang +11 more
wiley +1 more source
Advanced Science, EarlyView.Engineered phage‐based trifunctional nanofibers are developed for synergistic biofilm eradication. Biofilm‐binding phages, armed with AuNPs and TCPP, serve as both warheads and scaffolds, endowing the phototherapy agents with biofilm‐anchoring ability. Upon light irradiation, AuNPs induce photothermal therapy, while TCPP generates 1O2 for photodynamic ...
Ying Cao +9 more
wiley +1 more source
Advanced Science, EarlyView.Using single‐cell and spatial transcriptomics, we identified key regulators that govern cell differentiation involved in parasite development and egg laying in Schistosoma japonicum. This comprehensive study establishes a foundational resource for understanding schistosome biology, deciphers the regulatory programs underlying cell differentiation and ...
Zhigang Lu +9 more
wiley +1 more source
Comparative survey of the relative impact of mRNA features on local ribosome profiling read density
Nature Communications, 2016 Ribosome profiling data can suffer from uneven coverage which hampers estimation of elongation rates. Connor et al.present an enhanced data smoothing method for Ribo-seq data and highlight significant variability in sequence determinants of ribosome ...
Patrick B. F. O’Connor +2 more
doaj +1 more source