Results 81 to 90 of about 47,955 (276)

Ribosome profiling the cell cycle: lessons and challenges [PDF]

Current Genetics, 2017
Understanding the causes and consequences of dynamic changes in the abundance and activity of cellular components during cell division is what most cell cycle studies are about. Here we focus on control of gene expression in the cell cycle at the level of translation.
Rodolfo, Aramayo, Michael, Polymenis
openaire   +2 more sources

Simple and inexpensive ribosome profiling analysis of mRNA translation.

, 2018
The development and application of ribosome profiling has markedly advanced our understanding of ribosomes and mRNA translation. The experimental approach, which relies on deep sequencing of ribosome-protected mRNA fragments generated by treatment of ...
Nicchitta, Christopher V, Shenolikar, Shirish, Reid, David W   +2 more
core   +1 more source

TRMT6‐Mediated m1A Modification of CDK9 mRNA Is a Dual‐Pronged Pathogenic Driver for HBV‐Related Hepatocellular Carcinoma

Advanced Science, EarlyView.
TRMT6‐mediated m1A modification in CDK9 mRNA enhances its mRNA stability and translation efficiency, thereby increasing the protein levels of CDK9. Upregulated CDK9 promotes the progression of HCC by elevating the levels of oncogenic factors including p‐STAT3, MCL1, and BCL‐2. On the other hand, CDK9 phosphorylates TARDBP at Ser254 to activate HBV core
Rui Zhang, Dandan Zong, Rui Liu, Yubo Wang, Qingqing Gu, Yao Yao, Wenfang Zheng, Mengmeng Yuan, Simeng Wang, Rongrong Cui, Daxu Li, Siwen Dang, Peng Hou   +12 more
wiley   +1 more source

RNA Helicase DDX21 Controls CD4+ T Cell Proliferation and Promotes Inflammatory Bowel Disease via Translational Control

Advanced Science, EarlyView.
ABSTRACT Inflammatory bowel disease (IBD) is characterized by dysregulated T cell responses. RNA helicases, including DExD‐box helicase 21 (DDX21), are pivotal in RNA metabolism, but their role in T cell‐mediated pathology during IBD remains unclear. Here, we demonstrate that DDX21 expression in CD4+ T cells correlates with cell cycle and translation ...
Yujuan Zhang, Chen Kan, Xinhui Yang, Yajuan Hao, Xuemin Cai, Mei Yang, Qianqian Zhang, Zhengting Wang, Ning Jiang, Lei Wang, Hua‐Bing Li, Jing Zhou   +11 more
wiley   +1 more source

A Streamlined Ribosome Profiling Protocol for the Characterization of Microorganisms

BioTechniques, 2015
Ribosome profiling is a powerful tool for characterizing in vivo protein translation at the genome scale, with multiple applications ranging from detailed molecular mechanisms to systems-level predictive modeling. Though highly effective, this intricate technique has yet to become widely used in the microbial research community.
Haythem Latif, Richard Szubin, Justin Tan, Elizabeth Brunk, Anna Lechner, Karsten Zengler, Bernhard O. Palsson   +6 more
openaire   +3 more sources

PRC2.1 Coordinates Peri‐Nucleolar H3K27me3‐Enriched Heterochromatin Organization and NPM1 Pentamerization to Maintain Nucleolar Integrity

Advanced Science, EarlyView.
PRC2.1(PCL2)‐coordinated H3K27me3‐enriched PNH establishes a spatial scaffold crucial for nucleolar integrity. As a crucial coordinator, PCL2 links PRC2.1 to chromatin organization and NPM1 assembly. This network‐based model reveals how chromatin modifications and nucleolar components cooperatively maintain nucleolar architecture, revealing novel ...
Lina Zhu, Wenqin Wang, Shuyun Chen, Gangyi Zhu, Zhen Wu, Yue Gu, Jingwen Xiong, Xu Zhang, Heng Liu, Kyoichi Isono, Youming Zhang, Jingjing Cao, Xiangzhi Li   +12 more
wiley   +1 more source

Icariin Enhances the Enzymatic Activity of N‐acetylgalactosaminidase to Augment Akkermansia Abundance in Gut Microbiota for Improved PD‐1 Blockade Efficacy in Tumor Suppression

Advanced Science, EarlyView.
Icariin promoted the growth of Akk by enhancing the activity of N‐acetylgalactosaminidase (Amuc_0920), which enhanced mucin utilization and provided a favorable nutrient environment for bacterial growth. This icariin‐mediated enrichment of Akk further reshaped the tumor microenvironment and promoted CD8+ T cell infiltration, ultimately synergizing with
Shuangying Qiao, Liu Yang, Haibang Hao, Qiuxia Ding, Feng Ding, Zheng Chen, Jinfang Zhang, Yun He, Meng Li, Jun Xu, Chao Wang, Aiping Lu, Fangfei Li   +12 more
wiley   +1 more source

Comparative survey of the relative impact of mRNA features on local ribosome profiling read density

Nature Communications, 2016
Ribosome profiling data can suffer from uneven coverage which hampers estimation of elongation rates. Connor et al.present an enhanced data smoothing method for Ribo-seq data and highlight significant variability in sequence determinants of ribosome ...
Patrick B. F. O’Connor, Dmitry E. Andreev, Pavel V. Baranov   +2 more
doaj   +1 more source

Following Ribosome Footprints to Understand Translation at a Genome Wide Level

Computational and Structural Biotechnology Journal, 2018
Protein translation is a key step in gene expression. The development of Ribosome Profiling has allowed the global analysis of this process at sub-codon resolution.
Guillermo Eastman, Pablo Smircich, José R. Sotelo-Silveira   +2 more
doaj   +1 more source

An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome [PDF]

, 2017
Recent advances in global translatome analysis technologies enable us to understand how translational regulation of gene expression modulates cellular functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to
Hyun Yong, Jin, Changchun, Xiao
openaire   +2 more sources