Results 91 to 100 of about 7,960 (219)
Next-level riboswitch development-implementation of Capture-SELEX facilitates identification of a new synthetic riboswitch. [PDF]
, 2019 The development of synthetic riboswitches has always been a challenge. Although a number of interesting proof-of-concept studies have been published, almost all of these were performed with the theophylline aptamer. There is no shortage of small molecule-
Braun, Johannes +8 more
core +1 more source
Nature Communications, 2023 Cell-free genetically encoded biosensors have been developed to detect small molecules and nucleic acids, but they have yet to be reliably engineered to detect proteins.
Grace E. Vezeau +2 more
doaj +1 more source
Evaluating riboswitch optimality
, 2019 Riboswitches are RNA elements that recognize diverse chemical and biomolecular inputs, and transduce this recognition process to genetic, fluorescent, and other engineered outputs using RNA conformational changes. These systems are pervasive in cellular biology and are a promising biotechnology with applications in genetic regulation and biosensing ...
Hannah, Wayment-Steele +3 more
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Riboswitches and the RNA World [PDF]
Cold Spring Harbor Perspectives in Biology, 2010 Riboswitches are structured noncoding RNA domains that selectively bind metabolites and control gene expression (Mandal and Breaker 2004a; Coppins et al. 2007; Roth and Breaker 2009). Nearly all examples of the known riboswitches reside in noncoding regions of messenger RNAs where they control transcription or translation.
openaire +2 more sources
A Synthetic Riboswitch to Regulate Haloarchaeal Gene Expression
Frontiers in Microbiology, 2021 In recent years, synthetic riboswitches have become increasingly important to construct genetic circuits in all three domains of life. In bacteria, synthetic translational riboswitches are often employed that modulate gene expression by masking the Shine-
Johannes Born +4 more
doaj +1 more source
Folding Kinetics of Riboswitch Transcriptional Terminators and Sequesterers
Entropy, 2013 To function as gene regulatory elements in response to environmental signals, riboswitches must adopt specific secondary structures on appropriate time scales.
Ben Sauerwine, Michael Widom
doaj +1 more source
Ligand-Directed Dynamics of Adenine Riboswitch Conformers [PDF]
, 2016 Riboswitches harness the structural and dynamic sophistication of RNA to coordinate specific ligand recognition with changes in gene expression. Design of molecules to manipulate riboswitch responses relies on our understanding of their RNA−ligand ...
Oksana Prychyna (2475709) +4 more
core +1 more source
ChemPlusChem, Volume 91, Issue 5, May 2026.We established light‐ and bioluminescence‐responsive systems that allow reversible conjugation and dissociation of proteins on the inner leaflet of asymmetric lipid–amphiphilic protein hybrid vesicles. These systems provide a versatile platform for precise spatiotemporal regulation and bioluminescence‐triggered protein release within synthetic vesicles.
Masato Suzuki, Koki Kamiya
wiley +1 more source
Riboswitches and Translation Control [PDF]
Cold Spring Harbor Perspectives in Biology, 2018 A growing collection of bacterial riboswitch classes is being discovered that sense central metabolites, coenzymes, and signaling molecules. Included among the various mechanisms of gene regulation exploited by these RNA regulatory elements are several that modulate messenger RNA (mRNA) translation. In this review, the mechanisms of riboswitch-mediated
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Microfluidic Screening of Electrophoretic Mobility Shifts Elucidates Riboswitch Binding Function [PDF]
, 2013 Riboswitches are RNA sensors that change conformation upon binding small molecule metabolites, in turn modulating gene expression. Our understanding of riboswitch regulatory function would be accelerated by a high-throughput, quantitative screening tool ...
Qin, Qian +13 more
core +1 more source