Results 71 to 80 of about 7,960 (219)

Cobalamin Riboswitches Are Broadly Sensitive to Corrinoid Cofactors to Enable an Efficient Gene Regulatory Strategy

mBio, 2022
In bacteria, many essential metabolic processes are controlled by riboswitches, gene regulatory RNAs that directly bind and detect metabolites. Highly specific effector binding enables riboswitches to respond to a single biologically relevant metabolite.
Kristopher J. Kennedy, Florian J. Widner, Olga M. Sokolovskaya, Lina V. Innocent, Rebecca R. Procknow, Kenny C. Mok, Michiko E. Taga   +6 more
doaj   +1 more source

Honeydew: A Fluorescent Aptamer for Low‐Cost Azo Food Dyes

ChemBioChem, Volume 27, Issue 14, 29 July 2026.
Light‐up RNA aptamers that bind nonfluorescent dyes and conditionally activate their fluorescence are powerful tools for RNA imaging and biomolecular sensing. A barrier to widespread adoption of this technology is a reliance on bespoke dyes prepared by labor‐intensive or costly custom synthesis.
Joseph M. Heili, Paul H. Mroch, Tanner G. Hoog, Orion M. Venero, Grace M. Gustafson, Katarzyna P. Adamala, Aaron E. Engelhart   +6 more
wiley   +1 more source

A spermidine riboswitch class in bacteria exploits a close variant of an aptamer for the enzyme cofactor S-adenosylmethionine

Cell Reports, 2023
Summary: Natural polyamines such as spermidine and spermine cations have characteristics that make them highly likely to be sensed by riboswitches, such as their general affinity to polyanionic RNA and their broad contributions to cell physiology ...
Hubert Salvail, Aparaajita Balaji, Adam Roth, Ronald R. Breaker   +3 more
doaj   +1 more source

CRISPR‐Enabled Point‐of‐Care Diagnostics for Early Cancer Detection

Aggregate, Volume 7, Issue 7, July 2026.
This review summarizes recent advances in CRISPR‐enabled point‐of‐care diagnostics for early cancer detection, covering Cas‐based recognition mechanisms, portable engineering platforms, and biomarker applications. It further highlights current challenges and future opportunities for translating sensitive, affordable, and accessible CRISPR diagnostics ...
Yuwei Pan, Qihang Xu, Carla Spatola Rossi, Zhugen Yang   +3 more
wiley   +1 more source

Riboswitches in unexpected places—A synthetic riboswitch in a protein coding region [PDF]

RNA, 2008
In natural and engineered systems, cis-RNA regulatory elements such as riboswitches are typically found within untranslated regions rather than within the protein coding sequences of genes. However, RNA sequences with important regulatory roles can exist within translated regions.
Shana, Topp, Justin P, Gallivan
openaire   +2 more sources

Delivery Systems for Therapeutic Genome Editing: Challenges, Innovations, and Future Perspectives

MedComm, Volume 7, Issue 7, July 2026.
Schematic illustration of four emerging CRISPR–Cas delivery platforms defined by distinct design principles and structural features: virus‐mimicking nanosystems (e.g., VLPs), cell‐derived extracellular vesicles, cell‐penetrating peptides, and stimuli‐responsive scaffolds. These platforms enable spatiotemporally controlled delivery of RNPs, mRNA, or DNA
Meijia Yang, Yiqiong Song, Ziyang Wang, Ke Chao, Lifeng Li, Xu Zhang, Xiaoran Duan, Chenglong Yu, Ruyue Xue, Jie Zhao   +9 more
wiley   +1 more source

Riboswitches: Fold and Function

Chemistry & Biology, 2006
Riboswitches in the 5'-untranslated regions of mRNAs cotranscriptionally couple ligand binding and gene regulation. In this issue of Chemistry & Biology, Lemay et al. describe folding of a key tertiary interaction in the adenine riboswitch and its mechanistic consequences.
Gilbert, Sunny D., Batey, Robert T.
openaire   +2 more sources

Singlet glycine riboswitches bind ligand as well as tandem riboswitches [PDF]

RNA, 2016
The glycine riboswitch often occurs in a tandem architecture, with two ligand-binding domains (aptamers) followed by a single expression platform. Based on previous observations, we hypothesized that “singlet” versions of the glycine riboswitch, which contain only one aptamer domain, are able to bind glycine if appropriate structural contacts are ...
Ruff, Karen M., Muhammad, Ayesha, McCown, Phillip J., Breaker, Ronald R., Strobel, Scott A.   +4 more
openaire   +2 more sources

Dynamic energy landscapes of riboswitches help interpret conformational rearrangements and function. [PDF]

PLoS Computational Biology, 2012
Riboswitches are RNAs that modulate gene expression by ligand-induced conformational changes. However, the way in which sequence dictates alternative folding pathways of gene regulation remains unclear.
Giulio Quarta, Ken Sin, Tamar Schlick
doaj   +1 more source

Naturally Occurring CodY Variants Alter Ligand Binding, DNA Target Affinity, and Virulence in Clostridioides difficile

Molecular Microbiology, Volume 126, Issue 1, Page 98-110, July 2026.
Single amino acid substitutions in the global regulator CodY can alter nutrient sensing and virulence regulation in Clostridioides difficile. We show that the CodY(Y146N) and CodY(V58A) variants display altered ligand binding and reduced promoter binding, leading to derepression of toxin regulatory pathways and increased virulence in vivo.
Md Kamrul Hasan, Sourav Roy, Revathi Govind   +2 more
wiley   +1 more source