Results 51 to 60 of about 7,522 (226)
RSC Chemical Biology, 2021 Synthetic riboswitches can be used as chemical gene switches in cell-free protein synthesis systems. We provide a current perspective on the state of cell-free riboswitch technologies and their future directions.
Takeshi Tabuchi, Yohei Yokobayashi
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Alkyltransferase Ribozyme for Site‐Specific N4‐Cytidine Alkylation
Angewandte Chemie, Volume 138, Issue 20, 11 May 2026.CSAR is the first ribozyme that catalyzes direct alkylation of an exocyclic amino group of an RNA nucleobase. The ribozyme was isolated as a self‐labeling catalyst from an in vitro selection library and engineered into a trans‐active ribozyme that catalyzes the transfer of a benzyl group from an O6‐modifed guanine to the N4 amino group of a specific ...
Evgeniia Dorinova +2 more
wiley +2 more sources
Incoherent dual regulation by a SAM-II riboswitch controlling translation at a distance
RNA Biology, 2022 In Sinorhizobium meliloti, the methionine biosynthesis genes metA and metZ are preceded by S-adenosyl-L-methionine (SAM) riboswitches of the SAM-II class. Upon SAM binding, structural changes in the metZ riboswitch were predicted to cause transcriptional
Robina Scheuer +5 more
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Ligand response of guanidine-IV riboswitch at single-molecule level
eLifeRiboswitches represent a class of non-coding RNA that possess the unique ability to specifically bind ligands and, in response, regulate gene expression.
Lingzhi Gao, Dian Chen, Yu Liu
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TaRTLEt: Transcriptionally-active Riboswitch Tracer Leveraging Edge deTection [PDF]
PeerJStructured RNAs have emerged as a major component of cellular regulatory systems, but their mechanism of action is often poorly understood. Riboswitches are structured RNAs that allosterically regulate gene expression through any of several different ...
Sachit Kshatriya, Sarah C. Bagby
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Riboswitches: Structures and Mechanisms [PDF]
Cold Spring Harbor Perspectives in Biology, 2010 A critical feature of the hypothesized RNA world would have been the ability to control chemical processes in response to environmental cues. Riboswitches present themselves as viable candidates for a sophisticated mechanism of regulatory control in RNA-based life.
Andrew D, Garst +2 more
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Bifidobacterium bifidum SAM-VI Riboswitch Conformation Change Requires Peripheral Helix Formation
BiomoleculesThe Bifidobacterium bifidum SAM-VI riboswitch undergoes dynamic conformational changes that modulate downstream gene expression. Traditional structural methods such as crystallography capture the bound conformation at high resolution, and additional ...
Wenwen Xiao +4 more
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Folding of the Adenine Riboswitch
Chemistry & Biology, 2006 The pbuE adenine riboswitch undergoes metal ion-dependent folding that involves a loop-loop interaction. Binding of 2-aminopurine to the aptamer domain strongly correlates with the ability of the loops to interact, and single-molecule FRET studies reveal that folding proceeds via a discrete intermediate. Folding occurs in the absence of adenine ligand,
Lemay, Jean-François +4 more
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An in vivo high-throughput screening for riboswitch ligands using a reverse reporter gene system
Scientific Reports, 2017 Riboswitches are bacterial RNA elements that regulate gene expression in response to metabolite or ion abundance and are considered as potential drug targets. In recent years a number of methods to find non-natural riboswitch ligands have been described.
Marion Kirchner +3 more
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mBio, 2022 In bacteria, many essential metabolic processes are controlled by riboswitches, gene regulatory RNAs that directly bind and detect metabolites. Highly specific effector binding enables riboswitches to respond to a single biologically relevant metabolite.
Kristopher J. Kennedy +6 more
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