Results 41 to 50 of about 7,960 (219)

Architectures and complex functions of tandem riboswitches

RNA Biology, 2022
Riboswitch architectures that involve the binding of a single ligand to a single RNA aptamer domain result in ordinary dose-response curves that require approximately a 100-fold change in ligand concentration to cover nearly the full dynamic range for ...
Madeline E. Sherlock, Gadareth Higgs, Diane Yu, Danielle L. Widner, Neil A. White, Narasimhan Sudarsan, Harini Sadeeshkumar, Kevin R. Perkins, Gayan Mirihana Arachchilage, Sarah N. Malkowski, Christopher G. King, Kimberly A. Harris, Glenn Gaffield, Ruben M. Atilho, Ronald R. Breaker   +14 more
doaj   +1 more source

Clock and riboswitch control of THIC in tandem are essential for appropriate gauging of TDP levels under light/dark cycles in Arabidopsis

iScience, 2023
Summary: Metabolic homeostasis is regulated by enzyme activities, but the importance of regulating their corresponding coenzyme levels is unexplored. The organic coenzyme thiamine diphosphate (TDP) is suggested to be supplied as needed and controlled by ...
Zeenat Noordally, Lara Land, Celso Trichtinger, Ivan Dalvit, Mireille de Meyer, Kai Wang, Teresa B. Fitzpatrick   +6 more
doaj   +1 more source

The intricate world of riboswitches [PDF]

Current Opinion in Microbiology, 2007
Riboswitches are segments of the 5'-untranslated region of certain bacterial mRNAs that upon recognition of specific ligands modify the expression of a protein(s) encoded in the message. These proteins are responsible for the biosynthesis or transport of ligands, which are typically organic molecules but could also be metal ions.
Rebecca L, Coppins, Kathleen B, Hall, Eduardo A, Groisman   +2 more
openaire   +2 more sources

Mechanistic insights into an engineered riboswitch: a switching element which confers riboswitch activity [PDF]

, 2010
While many different RNA aptamers have been identified that bind to a plethora of small molecules only very few are capable of acting as engineered riboswitches.
Tristan J. Will, Weigand, Julia E., Weigand, Julia, Christian Hammann, Schmidtke, S. R., Hammann, Christian, Julia E. Weigand, Will, Tristan J., Wöhnert, Jens, Suess, Beatrix, Wöhnert, J., Schmidtke, Sina R., Sina R. Schmidtke, Elke Duchardt-Ferner, Jens Wöhnert, Will, T. J., Duchardt-Ferner, Elke, Süß, Beatrix, Beatrix Suess, Duchardt-Ferner, E.   +19 more
core   +1 more source

Single-molecule chemical denaturation of riboswitches [PDF]

, 2013
To date, single-molecule RNA science has been developed almost exclusively around the effect of metal ions as folding promoters and stabilizers of the RNA structure.
St-Pierre, Patrick, Penedo, J Carlos, Dubé, Audrey, Samuel, Ifor D W, St-Pierre, P, Samuel, Ifor David William, Lafontaine, Daniel, Dalgarno, Paul Allan, Bordello, Jorge, Morris, Rhodri, Bordello, J, Penedo, Carlos, Lafontaine, Daniel A, Dubé, A., Dalgarno, Paul A; id_orcid   +14 more
core   +1 more source

An Intrinsic Alkalization Circuit Turns on mntP Riboswitch under Manganese Stress in Escherichia coli

Microbiology Spectrum, 2022
The trace metal manganese in excess affects iron-sulfur cluster and heme-protein biogenesis, eliciting cellular toxicity. The manganese efflux protein MntP is crucial to evading manganese toxicity in bacteria.
Arunima Kalita, Rajesh Kumar Mishra, Vineet Kumar, Amit Arora, Dipak Dutta   +4 more
doaj   +1 more source

Classification of Riboswitch Families Using Block Location-Based Feature Extraction (BLBFE) Method [PDF]

Advanced Pharmaceutical Bulletin, 2020
Purpose: Riboswitches are special non-coding sequences usually located in mRNAs’ un-translated regions and regulate gene expression and consequently cellular function. Furthermore, their interaction with antibiotics has been recently implicated.
Faegheh Golabi, Mousa Shamsi, Mohammad Hosein Sedaaghi, Abolfazl Barzegar, Mohammad Saeid Hejazi   +4 more
doaj   +1 more source

The k-junction motif in RNA structure [PDF]

, 2014
The k-junction is a structural motif in RNA comprising a three-way helical junction based upon kink turn (k-turn) architecture. A computer program written to examine relative helical orientation identified the three-way junction of the Arabidopsis TPP ...
Daldrop, Peter, Jia Wang, Lilley, David M. J.; id_orcid, Peter Daldrop, David M. J. Lilley, Wang, Jia, Huang, Lin, Lin Huang   +7 more
core   +1 more source

Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products

BMC Research Notes, 2018
Objective The purpose of this project was to use an in vivo method to discover riboswitches that are activated by new ligands. We employed phage-assisted continuous evolution (PACE) to evolve new riboswitches in vivo.
Zachary M. Shaver, Stephanie S. Bent, Steven R. Bilby, Michael Brown, Anna Buser, Itzayana G. Cuellar, Athena J. Davis, Lindsay Doolan, Fatima C. Enriquez, Autumn Estrada, Shelby Herner, J. Cody Herron, Andrew M. Hunn, Madison Hunter, Hartlee Johnston, Owen Koucky, Christian C. Mackley, Dylan Maghini, Devin Mattoon, Haden T. McDonald, Hannah Sinks, Austin J. Sprague, David Sullivan, Altan Tutar, Avery Umphreys, Chris Watson, Daniel Zweerink, Laurie J. Heyer, Jeffrey L. Poet, Todd T. Eckdahl, A. Malcolm Campbell   +30 more
doaj   +1 more source

Magnesium ions mediate ligand binding and conformational transition of the SAM/SAH riboswitch

Communications Biology, 2023
The SAM/SAH riboswitch binds S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) with similar affinities. Mg2+ is generally known to stabilize RNA structures by neutralizing phosphates, but how it contributes to ligand binding and conformational ...
Guodong Hu, Huan-Xiang Zhou
doaj   +1 more source