Interplay of 'induced fit' and preorganization in the ligand induced folding of the aptamer domain of the guanine binding riboswitch [PDF]
, 2006 Riboswitches are highly structured elements in the 50-untranslated regions (50-UTRs) of messenger RNA that control gene expression by specifically binding to small metabolite molecules. They consist of an aptamer domain responsible for ligand binding and
Wöhnert, Jens +5 more
core +1 more source
Breaking Riboswitch Speed Limits by Systematic Mutation and Orthogonal Base Pair Incorporation [PDF]
, 2023 Engineered RNAs have applications in diverse fields, from biomedical to environmental. In many cases, folding of the RNA is critical to its function. Here we describe a strategy to improve the folding kinetics of a riboswitch-based fluorescent biosensor.
Praneeth, Bommisetti +6 more
core +1 more source
A riboswitch-based inducible gene expression system for mycobacteria. [PDF]
PLoS ONE, 2012 Research on the human pathogen Mycobacterium tuberculosis (Mtb) would benefit from novel tools for regulated gene expression. Here we describe the characterization and application of a synthetic riboswitch-based system, which comprises a mycobacterial ...
Jessica C Seeliger +5 more
doaj +1 more source
A transient conformation facilitates ligand binding to the adenine riboswitch
iScience, 2021 Summary: RNAs adopt various conformations to perform different functions in cells. Incapable of acquiring intermediates, the key initiations of ligand recognition in the adenine riboswitch have not been characterized.
Lin Wu, Dian Chen, Jienyu Ding, Yu Liu
doaj +1 more source
Influence of ground-state structure and Mg2+ binding on folding kinetics of the guanine-sensing riboswitch aptamer domain [PDF]
, 2011 Riboswitch RNAs fold into complex tertiary structures upon binding to their cognate ligand. Ligand recognition is accomplished by key residues in the binding pocket.
Julia Wirmer-Bartoschek +17 more
core +1 more source
Molecular dynamics simulation study of the binding of purine bases to the aptamer domain of the guanine sensing riboswitch [PDF]
, 2009 Riboswitches are a novel class of genetic control elements that function through the direct interaction of small metabolite molecules with structured RNA elements.
Stock, Gerhard +4 more
core +1 more source
PLoS ONE, 2020 Riboswitches, generally located in the 5'-leader of bacterial mRNAs, direct expression via a small molecule-dependent structural switch that informs the transcriptional or translational machinery.
Lea K Drogalis, Robert T Batey
doaj +1 more source
Translational gene expression control in Chlamydia trachomatis.
PLoS ONE, 2022 The human pathogen Chlamydia trachomatis proceeds through a multi phenotypic developmental cycle with each cell form specialized for different roles in pathogenesis.
Nicole A Grieshaber +5 more
doaj +1 more source
Mix-and-Match Riboswitches [PDF]
ACS Chemical Biology, 2006 Riboswitches are noncoding RNA elements found in the 5'-untranslated region of messenger RNA (mRNA) that mediate gene expression in a cis fashion in the absence of protein. This common regulatory strategy in bacteria is achieved through the interplay of two distinct domains: an aptamer domain responsible for sensing intracellular concentrations of a ...
Colby D, Stoddard, Robert T, Batey
openaire +2 more sources
Riboswitch-Based Reversible Dual Color Sensor [PDF]
, 2017 Riboswitches are RNA-based “sensors” that utilize chemically induced structural changes in the 5′-untranslated region of mRNA to regulate expression of downstream genes.
Nancy Kelley-Loughnane (1462666) +4 more
core +1 more source