Results 61 to 70 of about 194,357 (268)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Mechanical defenses of plant extrafloral nectaries against herbivory

Communicative & Integrative Biology, 2016
Extrafloral nectaries play an important role in plant defense against herbivores by providing nectar rewards that attract ants and other carnivorous insects.
Moshe Gish, Mark C. Mescher, Consuelo M. De Moraes   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Focal rigidity of flat tori

Anais da Academia Brasileira de Ciências, 2011
Given a closed Riemannian manifold (M, g), i.e. compact and boundaryless, there is a partition of its tangent bundle TM = ∪iΣi called the focal decomposition of TM.
Ferry Kwakkel, Marco Martens, Mauricio Peixoto   +2 more
doaj  

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Calculation of the residual bearing capacity of reinforced concrete beams by the rigidity (deflection) criterion

Инженерно-строительный журнал, 2015
The article proposes the method of calculating the bearing capacity of reinforced concrete beams at the operational stage by the rigidity (deflection) criterion. The methods, which were used in the article, are integral test and probabilistic methods for
V.S. Utkin, S.A. Solovyov
doaj   +1 more source

RIGIDITY OF THE SPINE [PDF]

The Journal of Nervous and Mental Disease, 1900
n ...
openaire   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

QT interval prolongation noted in one percent of 2553 Asian patients with schizophrenia: Findings from the REAP‐AP survey

Kaohsiung Journal of Medical Sciences, 2020
Although the association between antipsychotic use and corrected QT interval (QTc) prolongation has been repeatedly confirmed, the relationship has been rarely studied in a practical setting.
Seon‐Cheol Park, Bong Ju Lee, Jae Hong Park, Hiroaki Kawasaki, Ajit Avasthi, Sandeep Grover, Andi J. Tanra, Shih‐Ku Lin, Afzal Javed, Chay Hoon Tan, Norman Sartorius, Naotaka Shinfuku, Yong Chon Park   +12 more
doaj   +1 more source