Results 11 to 20 of about 488,336 (199)
Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces
BMC Research Notes, 2023 Objective Metatranscriptomic analysis of RNA viromes on built-environment surfaces is hampered by low RNA yields and high abundance of rRNA. Therefore, we evaluated the quality of libraries, efficiency of rRNA depletion, and viral detection sensitivity ...
Yuh Shiwa +5 more
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Comparison of DNA and RNA sequencing of total nucleic acids from human cervix for metagenomics
Scientific Reports, 2021 Although metagenomics and metatranscriptomics are commonly used to identify bacteria and viruses in human samples, few studies directly compare these strategies.
Laila Sara Arroyo Mühr +4 more
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In planta Transcriptome Analysis of Pseudomonas syringae
Bio-Protocol, 2018 Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv.
Tatsuya Nobori, Kenichi Tsuda
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Structural Biology of Bacterial RNA Polymerase
Biomolecules, 2015 Since its discovery and characterization in the early 1960s (Hurwitz, J. The discovery of RNA polymerase. J. Biol. Chem. 2005, 280, 42477–42485), an enormous amount of biochemical, biophysical and genetic data has been collected on bacterial RNA ...
Katsuhiko S. Murakami
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Nature Communications, 2021 In this study, Marlin et al. provide insights into the potential use of subunit vaccines that induce a high level of protection against SARS-CoV-2 in animal models.
Romain Marlin +37 more
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mSystems, 2023 Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė +10 more
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BMC Genomics, 2008 Background The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited.
Butcher Philip D +3 more
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Prediction of Novel Bacterial Small RNAs From RIL-Seq RNA–RNA Interaction Data
Frontiers in Microbiology, 2021 The genomic revolution and subsequent advances in large-scale genomic and transcriptomic technologies highlighted hidden genomic treasures. Among them stand out non-coding small RNAs (sRNAs), shown to play important roles in post-transcriptional ...
Amir Bar +3 more
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PLoS ONE, 2019 Sputum often contains large amounts of contaminating bacterial DNA that, if not eliminated during RNA isolation, may interfere with gene expression studies. During RNA isolation only repeated DNase treatment can effectively remove contaminating bacterial
Csilla Paska +3 more
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Host A-to-I RNA editing signatures in intracellular bacterial and single-strand RNA viral infections
Frontiers in Immunology, 2023 BackgroundMicrobial infection is accompanied by remodeling of the host transcriptome. Involvement of A-to-I RNA editing has been reported during viral infection but remains to be elucidated during intracellular bacterial infections.ResultsHerein we ...
Zhi-Yuan Wei +30 more
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