Results 21 to 30 of about 510,206 (266)

Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces

BMC Research Notes, 2023
Objective Metatranscriptomic analysis of RNA viromes on built-environment surfaces is hampered by low RNA yields and high abundance of rRNA. Therefore, we evaluated the quality of libraries, efficiency of rRNA depletion, and viral detection sensitivity ...
Yuh Shiwa, Tomoya Baba, Maria A. Sierra, JangKeun Kim, Christopher E. Mason, Haruo Suzuki   +5 more
doaj   +1 more source

Comparison of DNA and RNA sequencing of total nucleic acids from human cervix for metagenomics

Scientific Reports, 2021
Although metagenomics and metatranscriptomics are commonly used to identify bacteria and viruses in human samples, few studies directly compare these strategies.
Laila Sara Arroyo Mühr, Joakim Dillner, Agustin Enrique Ure, Karin Sundström, Emilie Hultin   +4 more
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RNA‐Seq for Bacterial Gene Expression [PDF]

Current Protocols in Nucleic Acid Chemistry, 2018
AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
Poulsen, Line Dahl, Vinther, Jeppe
openaire   +3 more sources

In planta Transcriptome Analysis of Pseudomonas syringae

Bio-Protocol, 2018
Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv.
Tatsuya Nobori, Kenichi Tsuda
doaj   +1 more source

RNA Dynamics in Aging Bacterial Spores [PDF]

Cell, 2012
Upon starvation, the bacterium Bacillus subtilis enters the process of sporulation, lasting several hours and culminating in formation of a spore, the most resilient cell type known. We show that a few days following sporulation, the RNA profile of spores is highly dynamic.
Segev, Einat, Smith, Yoav, Ben-Yehuda, Sigal   +2 more
openaire   +2 more sources

Structural Biology of Bacterial RNA Polymerase

Biomolecules, 2015
Since its discovery and characterization in the early 1960s (Hurwitz, J. The discovery of RNA polymerase. J. Biol. Chem. 2005, 280, 42477–42485), an enormous amount of biochemical, biophysical and genetic data has been collected on bacterial RNA ...
Katsuhiko S. Murakami
doaj   +1 more source

Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

BMC Genomics, 2008
Background The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited.
Butcher Philip D, Senner Claire, Laing Ken, Waddell Simon J   +3 more
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Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

Nature Communications, 2021
In this study, Marlin et al. provide insights into the potential use of subunit vaccines that induce a high level of protection against SARS-CoV-2 in animal models.
Romain Marlin, Veronique Godot, Sylvain Cardinaud, Mathilde Galhaut, Severin Coleon, Sandra Zurawski, Nathalie Dereuddre-Bosquet, Mariangela Cavarelli, Anne-Sophie Gallouët, Pauline Maisonnasse, Léa Dupaty, Craig Fenwick, Thibaut Naninck, Julien Lemaitre, Mario Gomez-Pacheco, Nidhal Kahlaoui, Vanessa Contreras, Francis Relouzat, Raphaël Ho Tsong Fang, Zhiqing Wang, Jerome Ellis, Catherine Chapon, Mireille Centlivre, Aurelie Wiedemann, Christine Lacabaratz, Mathieu Surenaud, Inga Szurgot, Peter Liljeström, Delphine Planas, Timothée Bruel, Olivier Schwartz, Sylvie van der Werf, Giuseppe Pantaleo, Mélanie Prague, Rodolphe Thiébaut, Gerard Zurawski, Yves Lévy, Roger Le Grand   +37 more
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Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment.

PLoS ONE, 2019
Sputum often contains large amounts of contaminating bacterial DNA that, if not eliminated during RNA isolation, may interfere with gene expression studies. During RNA isolation only repeated DNase treatment can effectively remove contaminating bacterial
Csilla Paska, Imre Barta, Orsolya Drozdovszky, Balazs Antus   +3 more
doaj   +1 more source

Interplay between bacterial 5′-NAD-RNA decapping hydrolase NudC and DEAD-box RNA helicase CsdA in stress responses

mSystems, 2023
Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė, Renatas Krasauskas, Kotryna Kvederavičiūtė, Gytė Tupikaitė, Aleksandr Osipenko, Algirdas Kaupinis, Monika Jazdauskaitė, Raminta Mineikaitė, Mindaugas Valius, Viktoras Masevičius, Giedrius Vilkaitis   +10 more
doaj   +1 more source