Results 31 to 40 of about 532,169 (312)

RNA Dynamics in Aging Bacterial Spores [PDF]

Cell, 2012
Upon starvation, the bacterium Bacillus subtilis enters the process of sporulation, lasting several hours and culminating in formation of a spore, the most resilient cell type known. We show that a few days following sporulation, the RNA profile of spores is highly dynamic.
Segev, Einat, Smith, Yoav, Ben-Yehuda, Sigal   +2 more
openaire   +2 more sources

In planta Transcriptome Analysis of Pseudomonas syringae

Bio-Protocol, 2018
Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv.
Tatsuya Nobori, Kenichi Tsuda
doaj   +1 more source

RNA‐Seq for Bacterial Gene Expression [PDF]

Current Protocols in Nucleic Acid Chemistry, 2018
AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
Poulsen, Line Dahl, Vinther, Jeppe
openaire   +3 more sources

Membrane recognition and dynamics of the RNA Degradosome [PDF]

, 2015
RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Luisi, Ben, Syma Khalid (580907), Bouvier, Marie, Turlan, Catherine, Kebalo, J.M., Peyron, Pascale, Leonora Poljak, Peter J Bond, Peter J. Bond (688987), Strahl, H., Poljak, Leonora, Carpousis, A.J., Khalid, Syma, Ben F. Luisi (688991), Turlan, C., Henrik Strahl, Strahl, Henrik, Syma Khalid, Khalid, S., Jean-Marie Kebalo, Pascale Peyron, Henrik Strahl (688985), Catherine Turlan, Bouvier, M., Marie Bouvier, Agamemnon J. Carpousis (12453), Jean-Marie Kebalo (688988), Agamemnon J Carpousis, Pascale Peyron (239058), Leendert Hamoen (688990), Bond, Peter J., Bond, P.J., Ben F Luisi, Catherine Turlan (688986), Bond, Peter, Viollier, Patrick H., Marie Bouvier (260008), Hamoen, L.; id_orcid, Hamoen, Leendert, Luisi, Ben F., Carpousis, Agamemnon J., Peyron, P., Poljak, L., Luisi, B.F., Kebalo, Jean-Marie, Leendert Hamoen, Leonora Poljak (688989), Carpousis, Agamemnon, Hamoen, L.   +48 more
core   +1 more source

Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS [PDF]

, 2013
: OxyS and RprA are two small noncoding RNAs (sRNAs) that modulate the expression of rpoS, encoding an alternative sigma factor that activates transcription of multiple Escherichia coli stress-response genes.
Taylor, James E., Callaghan, Anastasia J., Helen A. Vincent, Phillips, Jack O., Cary, Peter, Vincent, H., Peter D. Cary, Sobott, F., Jack O. Phillips, Frank Sobott, Vincent, Helen A., Henderson, Charlotte A., Stone, Carlanne, Alessandra Casamento, Henderson, Charlotte, Carlanne M. Stone, Anastasia J. Callaghan, Darren M. Gowers, Cary, Peter D., Charlotte A. Henderson, James E.N. Taylor, Casamento, A., Callaghan, Anastasia, Phillips, Jack, Gowers, Darren, Casamento, Alessandra, Stone, Carlanne M., Sobott, Frank, Gowers, Darren M.   +28 more
core   +1 more source

Structural Biology of Bacterial RNA Polymerase

Biomolecules, 2015
Since its discovery and characterization in the early 1960s (Hurwitz, J. The discovery of RNA polymerase. J. Biol. Chem. 2005, 280, 42477–42485), an enormous amount of biochemical, biophysical and genetic data has been collected on bacterial RNA ...
Katsuhiko S. Murakami
doaj   +1 more source

Interplay between bacterial 5′-NAD-RNA decapping hydrolase NudC and DEAD-box RNA helicase CsdA in stress responses

mSystems, 2023
Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė, Renatas Krasauskas, Kotryna Kvederavičiūtė, Gytė Tupikaitė, Aleksandr Osipenko, Algirdas Kaupinis, Monika Jazdauskaitė, Raminta Mineikaitė, Mindaugas Valius, Viktoras Masevičius, Giedrius Vilkaitis   +10 more
doaj   +1 more source

Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

Nature Communications, 2021
In this study, Marlin et al. provide insights into the potential use of subunit vaccines that induce a high level of protection against SARS-CoV-2 in animal models.
Romain Marlin, Veronique Godot, Sylvain Cardinaud, Mathilde Galhaut, Severin Coleon, Sandra Zurawski, Nathalie Dereuddre-Bosquet, Mariangela Cavarelli, Anne-Sophie Gallouët, Pauline Maisonnasse, Léa Dupaty, Craig Fenwick, Thibaut Naninck, Julien Lemaitre, Mario Gomez-Pacheco, Nidhal Kahlaoui, Vanessa Contreras, Francis Relouzat, Raphaël Ho Tsong Fang, Zhiqing Wang, Jerome Ellis, Catherine Chapon, Mireille Centlivre, Aurelie Wiedemann, Christine Lacabaratz, Mathieu Surenaud, Inga Szurgot, Peter Liljeström, Delphine Planas, Timothée Bruel, Olivier Schwartz, Sylvie van der Werf, Giuseppe Pantaleo, Mélanie Prague, Rodolphe Thiébaut, Gerard Zurawski, Yves Lévy, Roger Le Grand   +37 more
doaj   +1 more source

Prediction of Novel Bacterial Small RNAs From RIL-Seq RNA–RNA Interaction Data

Frontiers in Microbiology, 2021
The genomic revolution and subsequent advances in large-scale genomic and transcriptomic technologies highlighted hidden genomic treasures. Among them stand out non-coding small RNAs (sRNAs), shown to play important roles in post-transcriptional ...
Amir Bar, Liron Argaman, Yael Altuvia, Hanah Margalit   +3 more
doaj   +1 more source

Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment.

PLoS ONE, 2019
Sputum often contains large amounts of contaminating bacterial DNA that, if not eliminated during RNA isolation, may interfere with gene expression studies. During RNA isolation only repeated DNase treatment can effectively remove contaminating bacterial
Csilla Paska, Imre Barta, Orsolya Drozdovszky, Balazs Antus   +3 more
doaj   +1 more source