Results 31 to 40 of about 819,809 (314)

RNA‐Seq for Bacterial Gene Expression [PDF]

Current Protocols in Nucleic Acid Chemistry, 2018
AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
Poulsen, Line Dahl, Vinther, Jeppe
openaire   +3 more sources

Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

Nature Communications, 2021
In this study, Marlin et al. provide insights into the potential use of subunit vaccines that induce a high level of protection against SARS-CoV-2 in animal models.
Romain Marlin, Veronique Godot, Sylvain Cardinaud, Mathilde Galhaut, Severin Coleon, Sandra Zurawski, Nathalie Dereuddre-Bosquet, Mariangela Cavarelli, Anne-Sophie Gallouët, Pauline Maisonnasse, Léa Dupaty, Craig Fenwick, Thibaut Naninck, Julien Lemaitre, Mario Gomez-Pacheco, Nidhal Kahlaoui, Vanessa Contreras, Francis Relouzat, Raphaël Ho Tsong Fang, Zhiqing Wang, Jerome Ellis, Catherine Chapon, Mireille Centlivre, Aurelie Wiedemann, Christine Lacabaratz, Mathieu Surenaud, Inga Szurgot, Peter Liljeström, Delphine Planas, Timothée Bruel, Olivier Schwartz, Sylvie van der Werf, Giuseppe Pantaleo, Mélanie Prague, Rodolphe Thiébaut, Gerard Zurawski, Yves Lévy, Roger Le Grand   +37 more
doaj   +1 more source

Quantifying microbial utilization of petroleum hydrocarbons in salt-marsh sediments using the ^(13)C content of bacterial rRNA [PDF]

, 2007
Natural remediation of oil spills is catalyzed by complex microbial consortia. Here we take a whole-community approach to investigate bacterial incorporation of petroleum hydrocarbons from a simulated oil spill.
Dekas, Anne E., Edwards, Katrina J., Kraunz, Kimberly S., Leavitt, William R., Pearson, Ann, Sessions, Alex L.   +5 more
core   +5 more sources

The C-terminal tail of the yeast mitochondrial transcription factor Mtf1 coordinates template strand alignment, DNA scrunching and timely transition into elongation [PDF]

, 2020
Mitochondrial RNA polymerases depend on initiation factors, such as TFB2M in humans and Mtf1 in yeast Saccharomyces cerevisiae, for promoterspecific transcription.
Basu, Urmimala, Cho, Hayoon, Deshpande, Aishwarya, Kim, Hajin, Lee, Seung-Won, Patel, Smita S., Shen, Jiayu, Sohn, Byeong-Kwon   +7 more
core   +1 more source

RNA Dynamics in Aging Bacterial Spores [PDF]

Cell, 2012
Upon starvation, the bacterium Bacillus subtilis enters the process of sporulation, lasting several hours and culminating in formation of a spore, the most resilient cell type known. We show that a few days following sporulation, the RNA profile of spores is highly dynamic.
Segev, Einat, Smith, Yoav, Ben-Yehuda, Sigal   +2 more
openaire   +2 more sources

Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

BMC Genomics, 2008
Background The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited.
Butcher Philip D, Senner Claire, Laing Ken, Waddell Simon J   +3 more
doaj   +1 more source

Interplay between bacterial 5′-NAD-RNA decapping hydrolase NudC and DEAD-box RNA helicase CsdA in stress responses

mSystems, 2023
Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė, Renatas Krasauskas, Kotryna Kvederavičiūtė, Gytė Tupikaitė, Aleksandr Osipenko, Algirdas Kaupinis, Monika Jazdauskaitė, Raminta Mineikaitė, Mindaugas Valius, Viktoras Masevičius, Giedrius Vilkaitis   +10 more
doaj   +1 more source

Protocol for Metatranscriptomic analysis of Intestinal Microbiota [PDF]

, 2012
The objective of this publication is to provide the detailed protocol for metartancriptomisc studies of animal intestinal microbiota. The protocol describes isolation of high quality microbial community RNA from the mammalian intestinal content ...
Alvaro Hernandez, Donald Liu, John Alverdy, Valeriy Poroyko, Valeriy Poroyko   +4 more
core   +2 more sources

Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment.

PLoS ONE, 2019
Sputum often contains large amounts of contaminating bacterial DNA that, if not eliminated during RNA isolation, may interfere with gene expression studies. During RNA isolation only repeated DNase treatment can effectively remove contaminating bacterial
Csilla Paska, Imre Barta, Orsolya Drozdovszky, Balazs Antus   +3 more
doaj   +1 more source

Prediction of Novel Bacterial Small RNAs From RIL-Seq RNA–RNA Interaction Data

Frontiers in Microbiology, 2021
The genomic revolution and subsequent advances in large-scale genomic and transcriptomic technologies highlighted hidden genomic treasures. Among them stand out non-coding small RNAs (sRNAs), shown to play important roles in post-transcriptional ...
Amir Bar, Liron Argaman, Yael Altuvia, Hanah Margalit   +3 more
doaj   +1 more source